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We narrowed to 5,240 results for: codon optimized

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Showing: 5041 - 5060 of 5240 results

  1. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

    Plasmid
    #228251
    Purpose
    pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228251-plasmid-map-sequence-id-451023

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  2. pCR8/GW_TOPO_Cdk13_Nterm_FlagHa

    Plasmid
    #127343
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into Gateway entry vector
    Depositor
    Phil Sharp
    Article
    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.
    Insert
    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    Use
    Entry vector with transgene for gateway cloning
    Tags
    Flag- HA- Tandem Epitopes
    Mutation
    Base pairs 1-1554 of transgene are codon optimize…
    Promoter
    None
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    127343-plasmid-map-sequence-id-246564

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  3. PB-Neo-TetO-Cdk13_Untagged

    Plasmid
    #127344
    Purpose
    Untagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Insco et al Science. 2023 Apr 21;380(6642):eabn7625.
    Insert
    Cyclin Dependent Kinase 13 (Cdk13 Mouse)
    Use
    Piggybac transposase vector
    Tags
    None
    Expression
    Mammalian
    Mutation
    Base pairs 1-1554 of transgene are codon optimize…
    Promoter
    TetO Promoter
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    127344-plasmid-map-sequence-id-246520

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  6. pUt-mNF-BACH1

    Plasmid
    #199219
    Purpose
    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 
    Depositor
    Gabor Balazsi
    Article
    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d
    Insert
    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV D2ir promoter
    Available Since
    Aug. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    199219-plasmid-map-sequence-id-396467

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  10. pX020

    Plasmid
    #167146
    Purpose
    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi caffeoylpiruvate nnCPH codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal caffeoylpiruvate hydrolase, nnCPH
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167146-plasmid-map-sequence-id-335229

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  11. pX131

    Plasmid
    #167149
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding Aspergillus nidulans 4'-phosphopantetheinyl transferase NpgA codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    4'-phosphopantetheinyl transferase, npgA
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167149-plasmid-map-sequence-id-335232

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  12. pX018

    Plasmid
    #167144
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding Neonothopanus nambi luciferase nnLuz codon-optimised for expression in Nicotiana benthamiana and Pichia pastoris
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal luciferase, nnLuz
    Use
    Luciferase and Synthetic Biology
    Available Since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167144-plasmid-map-sequence-id-335106

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  13. pX019

    Plasmid
    #167145
    Purpose
    MoClo-compatible Level 0-CDS1 promoterless vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    fungal hispidin-3-hydroxylase, nnH3H
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167145-plasmid-map-sequence-id-335228

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  14. pX022

    Plasmid
    #167148
    Purpose
    MoClo-compatible Level 0-CDS2 promoterless vector encoding C-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    C-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167148-plasmid-map-sequence-id-335231

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  15. pX021

    Plasmid
    #167147
    Purpose
    MoClo-compatible Level 0-SP promoterless vector encoding N-terminal half of the Neonothopanus nambi hispidin synthase nnHispS codon-optimised for expression in Nicotiana benthamiana
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    N-part of fungal hispidin synthase, nnHispS
    Use
    Synthetic Biology
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167147-plasmid-map-sequence-id-335230

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  16. pNK2657

    Plasmid
    #219741
    Purpose
    MoClo-compatible Level 0 promoterless vector encoding hispidin-synthase from Mycena citricolor codon-optimised for expression in Nicotiana benthamiana, Pichia pastoris, Homo sapiens
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    hispidin- synthase from Mycena citricolor
    Use
    Synthetic Biology
    Available Since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219741-plasmid-map-sequence-id-437095

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Showing: 5041 - 5060 of 5240 results