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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,214 results for: plasmid dna

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Showing: 5041 - 5060 of 7214 results

  1. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-11

    Plasmid
    #129039
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA11 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA11 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129039-plasmid-map-sequence-id-256194

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  2. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-2

    Plasmid
    #129030
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA2 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA2 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129030-plasmid-map-sequence-id-256140

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  3. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-3

    Plasmid
    #129031
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA3 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA3 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129031-plasmid-map-sequence-id-256155

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  4. pSpdCas9-huTET1CD-T2A-mCherry(PX458)-SghuTSDR-4

    Plasmid
    #129032
    Purpose
    targeted DNA demethylation_human_TSDR, expression of dCas9-huTET1CD-T2A-mCherry and sgRNA4 targeting human TSDR
    Depositor
    Julia K Polansky
    Article
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Insert
    dCas9-huTET1CD, SgRNA4 (huTSDR)
    Tags
    HA-Tag, NLS and T2A-mCherry
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available Since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    129032-plasmid-map-sequence-id-256156

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  5. PVALB mAb _BCN14.1.1C9_HC_Mouse IgG2a

    Plasmid
    #182888
    Purpose
    Mammalian expression of PVALB mouse mAb HC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) heavy chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182888-plasmid-map-sequence-id-360592

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  6. PVALB mA_BCN14.1.1C9_LC_Mouse Ig Kappa

    Plasmid
    #182889
    Purpose
    Mammalian expression of PVALB mouse mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182889-plasmid-map-sequence-id-360569

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  7. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

    Plasmid
    #182891
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182891-plasmid-map-sequence-id-360625

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  8. PVALB mAb_BCN14.1.1A2_LC_Mouse Ig Kappa

    Plasmid
    #182893
    Purpose
    Mammalian expression of PVALB mouse mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Mouse)
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182893-plasmid-map-sequence-id-360573

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  11. KG#121

    Plasmid
    #110879
    Purpose
    Expresses the C. elegans unc-31 cDNA pan-neuronally
    Depositor
    Kenneth Miller
    Article
    Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1
    Inserts
    rab-3 promoter
    unc-31 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Mutation
    Missing first 408bp
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110879-plasmid-map-sequence-id-212481

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  12. KG#371

    Plasmid
    #110882
    Purpose
    Expresses the lysosome marker CTNS-1-mCherry cDNA in a subset of ventral cord cholinergic motor neurons
    Depositor
    Kenneth Miller
    Article
    Edwards et al Genetics. 2013 May;194(1):143-61. doi: 1
    Inserts
    unc-129 promoter
    CTNS-1a-mCherry cDNA
    unc-54 3' control region with 1 intron just upstream and 1 intron in control region
    Tags
    intron-mCherry
    Expression
    Bacterial
    Available Since
    June 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    110882-plasmid-map-sequence-id-212577

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  13. pHR_SFFV*/Puro2a-GFP

    Plasmid
    #198222
    Purpose
    Lentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFP
    Depositor
    Eric Davis
    Insert
    EGFP
    Use
    Lentiviral
    Tags
    Codon-optimized puromycin resistance gene, follow…
    Expression
    Mammalian
    Available Since
    April 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    198222-plasmid-map-sequence-id-389408

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  15. pgRNA-humanized

    Plasmid
    #44248
    Purpose
    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    guide RNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    mouse U6
    Available Since
    April 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44248-plasmid-map-sequence-id-190219

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  18. pHW1387

    Plasmid
    #229880
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses - Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Pmyo-2::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Tags
    GFP-C1
    Expression
    Worm
    Available Since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229880-plasmid-map-sequence-id-460861

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  19. pHW1392

    Plasmid
    #229878
    Purpose
    Negative selection marker for extrachromosomal arrays during RMCE crosses- Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Depositor
    Han Wang
    Article
    Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.
    Insert
    Psnt-1::HisCl1 cDNA::SL2::GFP-C1::tbb-2 3'UTR
    Tags
    GFP-C1
    Expression
    Worm
    Available Since
    Feb. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    229878-plasmid-map-sequence-id-460858

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Showing: 5041 - 5060 of 7214 results