We narrowed to 7,176 results for: cas9 plasmid

1. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. sgRNA4_NANOG

   Plasmid

   #64156

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA4_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. sgRNA3_NANOG

   Plasmid

   #64155

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. sgRNA2_NANOG

   Plasmid

   #64154

   Purpose

   Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_NANOG (NANOG Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Immune checkpoint (library 2) Backbone

   Plasmid

   #192363

   Purpose

   Plasmid vector for in vivo immune checkpoint screening library (for checkpoint library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. T cell activation (library 1) Backbone

   Plasmid

   #192364

   Purpose

   Plasmid vector for in vitro Jurkat activation screening (for immune library)

   Depositor

   Lijia Ma

   Article

   Lu et al Nucleic Acids Res. 2022 Jun 24;50(11):65

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-AcrIIA1-NLS(SV40) (KAC478)

   Plasmid

   #133795

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 with C-terminal NLS (SV40)

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Osuna et al Cell Host Microbe. 2020 Apr 18. pii: S19

   Insert

   human codon optimized AcrIIA1
   

   Use

   Tags

   NLS(SV40)

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   CMV and T7

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. NDUFAB1 C3.3 gRNA

   Plasmid

   #90791

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C3.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. NDUFAB1 C4.3 gRNA

   Plasmid

   #90793

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human NDUFAB1

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   NDUFAB1 (Guide Designation C4.3) (NDUFAB1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge