We narrowed to 15,981 results for: LEA

1. pcDNA3-JNKAR1-NES

   Plasmid

   #84632

   Purpose

   Unimolecular, FRET-based JNK activity reporter; cytosol targeted

   Depositor

   Jin Zhang

   Article

   Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

   Insert

   ECFP-FHA1-JNKsubDD-Citrine-NES
   

   Tags

   6xHis, Citrine, ECFP, Nuclear export signal (NES)…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pY013 (pcDNA3.1-hEeCpf1)

   Plasmid

   #69985

   Purpose

   Expresses humanized EeCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hEeCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

3. 3535 pET15b Bid p22

   Plasmid

   #8784

   Depositor

   Stanley Korsmeyer

   Article

   Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

   Insert

   Bid p22 (Bid Mouse)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1-CMV-CIBN-mCherry-VAMP2

   Plasmid

   #178573

   Purpose

   Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

   Depositor

   Won Do Heo

   Article

   Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

   Insert

   CIBN-mCherry-VAMP2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY019 (pcDNA3.1-hPmCpf1)

   Plasmid

   #69991

   Purpose

   Expresses humanized PmCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hPmCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pY009 (pcDNA3.1-hSsCpf1)

   Plasmid

   #69981

   Purpose

   Expresses humanized SsCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hSsCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pAYC

   Plasmid

   #62657

   Purpose

   Mammalian expression of iCre recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   iCre
   

   Expression

   Mammalian

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. R26-EGFP MMEJ donor vector

   Plasmid

   #137926

   Purpose

   R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-EGFP-bpA with micro-homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-pEF1a-FLEX-HA-VHH-KASH-WPRE

   Plasmid

   #129704

   Purpose

   Express HA-KASH for nuclei labeling and purification

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   HA-VHH-KASH
   

   Use

   AAV and Cre/Lox

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNIC-Bio2_SMAC(delta(55))_9His

    Plasmid

    #197976

    Purpose

    E. coli expression of SMAC(delta(55))-9His

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    Smac (DIABLO Human)
    

    Tags

    9His

    Expression

    Bacterial

    Mutation

    delta (1-55)

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3495 pcDNA3 Bid p15

    Plasmid

    #8780

    Depositor

    Stanley Korsmeyer

    Article

    Wei et al Genes Dev 2000 Aug 15;14(16):2060-71.

    Insert

    Bid p15 (Bid Mouse)
    

    Expression

    Mammalian

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

12. pY015 (pcDNA3.1-hLiCpf1)

    Plasmid

    #69987

    Purpose

    Expresses humanized LiCpf1

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    hLiCpf1
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pGEX4T-1 Srap1

    Plasmid

    #89691

    Purpose

    Expresses murine Srap1 in bacteria

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Murine SRAP domain ortholog Srap1 (Hmces Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. cycLuc_SuMMVs

    Plasmid

    #119210

    Purpose

    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)

    Depositor

    Roman Jerala

    Article

    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d

    Insert

    cyclic luciferase with SuMMVp cleavage site
    

    Use

    Luciferase

    Tags

    AU1 tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLaNGo11-1

    Plasmid

    #126053

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pICSL80036

    Plasmid

    #68259

    Purpose

    Level 0 Golden Gate Part: Coding sequence, hygromycin phophotransferase II (Escherichia coli)

    Depositor

    Nicola Patron

    Article

    Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

    Insert

    Coding sequence, hygromycin phophotransferase II (Escherichia coli)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS415-Cas9-VPR

    Plasmid

    #163971

    Purpose

    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Insert

    Cas9-VPR
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. dTomato_LD 0_CS G_tTA-BFP

    Plasmid

    #58871

    Purpose

    MESA target chain with dTomato ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHJ424

    Plasmid

    #20682

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Ezrin (EZR Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Lys 253, 254, 262, 263 to Asn

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

20. pIS1 IMP-1 long UTR

    Plasmid

    #21640

    Depositor

    David Bartel

    Article

    Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

    Insert

    IGF-II mRNA-binding protein 1 UTR (IGF2BP1 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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