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We narrowed to 6,563 results for: mag

Showing: 5101 - 5120 of 6563 results

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pFRT-TO-RPB1-His CRres K619,627,642R si2,4R

Plasmid

#139409

Purpose

Dox-inducible, K619, 627, 642R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K619, 627, 642R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K751,758,767R CRres si2,4R

Plasmid

#139408

Purpose

Dox-inducible, K751, 758, 767R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K751, 758, 767R

Promoter

Available since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFRT-TO-RPB1-His K710,719R CRres si2,4R

Plasmid

#139407

Purpose

Dox-inducible, K710, 719R C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

K710,719R

Promoter

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-CMV TIP60 212-364

Plasmid

#78795

Purpose

To overexpress TIP60 212-364 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

212-364, G214A

Promoter

CMV

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
Cherry-SRBC

Plasmid

#69492

Purpose

Expression of fluorescently tagged SRBC in mammalian cells

Depositor

Rob Parton

Article

Gambin et al Elife. 2013 Jan 1;3:e01434. doi: 10.7554

Insert

SRBC (Cavin3 Mouse)

Use

Tags

Cherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV TIP60 390-513

Plasmid

#78797

Purpose

To overexpress TIP60 390-513 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

aa390-513

Promoter

CMV

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
AL05_pDonor.DMD

Plasmid

#100284

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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AC62_pDonor.DMD.TS.DR

Plasmid

#100288

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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Desmoplakin II no force control (FL-based)

Plasmid

#118724

Purpose

The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.

Depositor

Carsten Grashoff

Article

Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:

Insert

human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)

Use

Retroviral

Tags

YPet(short)-FL-mCherry

Expression

Mammalian

Mutation

truncation after aa1353

Promoter

CMV

Available since

Dec. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-FLAG-RGS14

Plasmid

#236567

Purpose

Expresses Flag-RGS14 in mammalian cells

Depositor

John Hepler

Article

Friedman et al J Biol Chem. 2022 May;298(5):101836. doi

Insert

Homo sapiens regulator of G-protein signaling 14 (RGS14 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN_4A

Plasmid

#227435

Purpose

Expression of the R-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN 4A (Pten Rat)

Use

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CAG_G-PTEN

Plasmid

#227436

Purpose

Expresses the G-PTEN sensor under a CAG promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN sensor, mEGFP-PTEN-sREACh (Pten Rat)

Use

Tags

mEGFP, and sREACh

Expression

Mammalian

Mutation

R14G

Promoter

CAG

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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PX330-PTEN

Plasmid

#227440

Purpose

Cas9 expression and a gRNA targeting Exon 6 of mouse PTEN

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

spCas9 and gRNA for mouse PTEN (Pten )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_R-PTEN

Plasmid

#227433

Purpose

Expresses the R-PTEN sensor under a CMV promoter

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

R-PTEN (Pten Rat)

Use

Tags

mCyRFP2, mMaroon

Expression

Mammalian

Mutation

R14G

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV_G-PTEN_4A

Plasmid

#227434

Purpose

Expresses the G-PTEN sensor with a 4A mutation

Depositor

Tal Laviv

Article

Kagan et al Nat Methods. 2025 Apr;22(4):764-777. doi

Insert

G-PTEN 4A (Pten Rat)

Use

Tags

mEGFP and sREACh

Expression

Mammalian

Mutation

4A mutation.

Promoter

CMV

Available since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCpep-E1GFP

Plasmid

#233884

Purpose

Expression of a pH sensitive variant of EGFP inserted in the C-peptide of insulin for pH measurement in insulin secretory granules

Depositor

Francesco Cardarelli

Article

De Lorenzi et al Commun Biol. 2025 Feb 25;8(1):304. doi:

Insert

Proinsulin-E1GFP (Ins2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Inserted E1GFP into C-peptide of mouse Ins2 codin…

Promoter

CMVF

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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WT USP7 UBL-12

Plasmid

#227230

Purpose

Expresses human UBL-12 domain of USP7 with a N-terminal cleavable 6His tag in E. coli

Depositor

Irina Bezsonova

Article

Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

Insert

USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)

Use

Tags

6His

Expression

Bacterial

Mutation

aa 535-775

Promoter

T7-promoter

Available since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry-hsp70-zCreI-mTagBFP2 (JDW 1235)

Plasmid

#229814

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with hsp70-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

loxp-EGFP-stop-lox

Use

Cre/Lox; Tol2 based expression vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)

Plasmid

#229816

Purpose

A Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

loxp-EGFP-stop-lox

Use

Tol2 based expression vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

Plasmid

#229826

Purpose

Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

mScarlet-I

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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