We narrowed to 9,853 results for: otos

1. pCAGGS puro HA-hUSP27X S404N

   Plasmid

   #225724

   Purpose

   Transfection of USP27X (S404N) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X S404N (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S404N

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS puro HA-hUSP27X Y381H

   Plasmid

   #225723

   Purpose

   Transfection of USP27X (Y381H) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y381H (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y381H

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGGS puro HA-hUSP27X F313V

   Plasmid

   #225722

   Purpose

   Transfection of USP27X (F313V) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X F313V (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F313V

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS puro HA-hUSP27X K181E

   Plasmid

   #225721

   Purpose

   Transfection of USP27X (K181E) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X K181E (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K181E

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAGGS puro HA-hUSP27X Y144C

   Plasmid

   #225720

   Purpose

   Transfection of USP27X (Y144C) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y144C (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y144C

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCAGGS puro HA-hUSP27X G76S

   Plasmid

   #225719

   Purpose

   Transfection of USP27X (G76S) with HA tag in mammalian cells

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X G76S (USP27X Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   G76S

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX6P1 GST-hUSP27X S404N

   Plasmid

   #225717

   Purpose

   Bacterial expression of USP27X (S404N) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X S404N (USP27X Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   S404N

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX6P1 GST-hUSP27X Y381H

   Plasmid

   #225716

   Purpose

   Bacterial expression of USP27X (Y381H) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X Y381H (USP27X Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Y381H

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX6P1 GST-hUSP27X F313V

   Plasmid

   #225715

   Purpose

   Bacterial expression of USP27X (F313V) with GST tag

   Depositor

   Francisco Bustos

   Article

   Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

   Insert

   USP27X F313V (USP27X Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   F313V

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX6P1 GST-hUSP27X Y144C

    Plasmid

    #225713

    Purpose

    Bacterial expression of USP27X (Y144C) with GST tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X Y144C (USP27X Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Y144C

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX6P1 GST-hUSP27X G76S

    Plasmid

    #225712

    Purpose

    Bacterial expression of USP27X (G76S) with GST tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X G76S (USP27X Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    G76S

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX6P1 GST-hUSP27X C87A

    Plasmid

    #225711

    Purpose

    Bacterial expression of USP27X (C87A) with GST tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X C87A (USP27X Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    C87A, inactivates deubiquitylase

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKLV2.2-h7SKgRNA5(ARID1A(44))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

    Plasmid

    #200506

    Purpose

    Lentiviral vector expressing gRNA targeting human ARID1A and AAVS1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    ARID1A(44), AAVS1 (ARID1A, AAVS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(AAVS1)-PGKpuroBFP-W

    Plasmid

    #200503

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and AAVS1

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), AAVS1 (SMARCA4, AAVS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(46))-PGKpuroBFP-W

    Plasmid

    #200504

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), SMARCA2(46) (SMARCA2, SMARCA4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKLV2.2-h7SKgRNA5(SMARCA4(43))-hU6gRNA5(SMARCA2(47))-PGKpuroBFP-W

    Plasmid

    #200505

    Purpose

    Lentiviral vector expressing gRNA targeting human SMARCA4 and SMARCA2

    Depositor

    Kosuke Yusa

    Article

    Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

    Insert

    SMARCA4(43), SMARCA2(47) (SMARCA4, SMARCA2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUt-mNF-BACH1

    Plasmid

    #199219

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with GFP-BACH1 Negative-Feedback circuit (mNF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    hTetR::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV D2ir promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PB-TAC-OKMS

    Plasmid

    #80480

    Purpose

    piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [10-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)

    Plasmid

    #112022

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO alpha/beta into TRBC1 HDRDT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28 E2 LBD-Tev-E1a-His

    Plasmid

    #232117

    Purpose

    For bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.

    Depositor

    Ruth Sommese

    Article

    Liu et al J Biol Chem. 2023 Mar;299(3):102959. doi

    Insert

    DBT (DBT Human)
    

    Use

    Tags

    6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. NYESO alpha into TRAC HDRT Source (pTR 223)

    Plasmid

    #112023

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NYESO beta into TRBC1 HDRT Source (pTR 277)

    Plasmid

    #112024

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta into TRBC1 HDRT
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE

    Plasmid

    #202198

    Purpose

    Expresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotor

    Depositor

    Ofer Yizhar

    Article

    Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

    Insert

    stChrimsonR, PdCO
    

    Use

    AAV

    Tags

    EGFP and Rho1D4

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIα minimal promotor (0.4kb)

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pUt-mPF-BACH1 Cas9-Resistant_AmpR

    Plasmid

    #199221

    Purpose

    LPUtopia-7 matching RMCE donor plasmid with Cas9-resistant GFP-BACH1 Positive-Feedback circuit (mPF-BACH1). Use BlastR for positive selection and HSV-TK for negative selection. 

    Depositor

    Gabor Balazsi

    Article

    Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

    Insert

    rtTA-Advanced::P2A::EGFP::BACH1 (BACH1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    silient mutation at nucleic acid 177 C changed to…

    Promoter

    tight TRE promoter

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB-TAC-OK+9MS

    Plasmid

    #80482

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

    Depositor

    Knut Woltjen

    Article

    Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

    Insert

    OK+9MS (Pou5f1, Klf4, Myc, Sox2 Mouse)
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Promoter

    tetO

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFP-N1-HSD17B11-V16D-FLAGC

    Plasmid

    #184500

    Purpose

    Expresses the V16D mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    17-beta-hydroxysteroid dehydrogenase 11 (HSD17B11 Human)
    

    Use

    Tags

    FLAGC-GFP

    Expression

    Mammalian

    Mutation

    V16D

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEGFP-N1-HSD17B11-L14P-FLAGC

    Plasmid

    #184499

    Purpose

    Expresses the L14P mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    17-beta-hydroxysteroid dehydrogenase 11 (HSD17B11 Human)
    

    Use

    Tags

    FLAGC-GFP

    Expression

    Mammalian

    Mutation

    L14P

    Promoter

    CMV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pEGFP-N1-HSD17B11-S172L-FLAGC

    Plasmid

    #161904

    Purpose

    Expresses the catalytically inactive S172L mutant of human HSD17B11 with a C-terminal FLAG-GFP tag. Confers G418 resistance.

    Depositor

    Sébastien Britton

    Article

    Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

    Insert

    hydroxysteroid 17-beta dehydrogenase 11 (HSD17B11 Human)
    

    Use

    Tags

    FLAG-GFP

    Expression

    Mammalian

    Mutation

    S172L; catalytically dead mutant

    Promoter

    CMV

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19-HDRT-CD3z-truncCARgsg(anti-CD19)

    Plasmid

    #215759

    Purpose

    HDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2 (enhanced expression by GSG-2A linker)

    Depositor

    Dimitrios Wagner

    Article

    Kath et al Blood. 2024 Mar 17:blood.2023020973. doi

    Insert

    CD247-truncCD19CAR-GSG (CD19, CD247 Human)
    

    Use

    Tags

    IgG1 CH3

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge