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pcDNA3-FLAG3-drAgo2D-E

Plasmid

#221731

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Mutation

D651E in active site

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG3-drAgo2

Plasmid

#221730

Purpose

Expression of zebrafish Ago2 with N-terminal 3xFLAG tag in mammalian cells.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2EF-DY

Plasmid

#221723

Purpose

Expression of human Ago2 with E637D and F666Y mutations and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2EF-DY (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D and F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2F-Y

Plasmid

#221722

Purpose

Expression of human Ago2 with F666Y mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2F-Y (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

F666Y in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2E-D

Plasmid

#221721

Purpose

Expression of human Ago2 with E637D mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2E-D (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

E637D in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2D-A

Plasmid

#221720

Purpose

Expression of human Ago2 with D669A mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2D-A (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Mutation

D669A in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-hsAgo2

Plasmid

#221719

Purpose

Expression of human Ago2 with N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Tags

3xFLAG

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-hsAgo2

Plasmid

#221718

Purpose

Expression of human Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

hsAgo2 (AGO2 Human)

Use

Fish expression

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-FLAG3-drAgo2Y-F

Plasmid

#221715

Purpose

Expression of zebrafish Ago2 with Y680F mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2Y-F (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

Y680F in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCS2+-FLAG3-drAgo2D-E

Plasmid

#221714

Purpose

Expression of zebrafish Ago2 with D651E mutation and N-terminal 3xFLAG tag in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2D-E (ago2 Zebrafish)

Use

Fish expression

Tags

3xFLAG

Mutation

D651E in active site

Promoter

simian CMV IE94

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-drAgo2

Plasmid

#221711

Purpose

Expression of zebrafish Ago2 in zebrafish embryos.

Depositor

David Bartel

Article

Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5

Insert

drAgo2 (ago2 Zebrafish)

Use

Fish expression

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-erUnaG

Plasmid

#221663

Purpose

For Agrobacterium-mediated transformation. Endoplasmic reticulum-localized mCherry-UnaG.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

Insert

mCherry-UnaG flanked with an N-terminal signal peptide or field pumpkin 25 albumin

Expression

Plant

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-oeUnaG

Plasmid

#221662

Purpose

For Agrobacterium-mediated transformation. Plastid(outer envelope membrane)-localized mCherry-UnaG.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

Insert

mCherry-UnaG flanked with an N-terminal sequence of Arabidopsis OUTRE ENVELOPE PROTEIN7

Expression

Plant

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-ptUnaG

Plasmid

#221660

Purpose

For Agrobacterium-mediated transformation. Plastid(stroma)-localized mCherry-UnaG.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

Insert

mCherry-UnaG

Expression

Plant

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-mtUnaG

Plasmid

#221664

Purpose

For Agrobacterium-mediated transformation. Mitochondria-localized mCherry-UnaG

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

Insert

mCherry-UnaG flanked with an N-terminal presequence of F1-ATPase γ-subunit

Expression

Plant

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB602-tkUnaG

Plasmid

#221661

Purpose

For Agrobacterium-mediated transformation. Plastid(thylakoid membrane)-localized mCherry-UnaG.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Physiol. 2024 Feb 27:pcae017.

Insert

mCherry-UnaG flanked with the full-length coding sequence for Arabidopsis BESTROPHIN-LIKE PROTEIN2

Expression

Plant

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-CAMK2D

Plasmid

#221686

Purpose

Mammalian expression of Myc-tagged BirA*-CAMK2D fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

CAMK2D (CAMK2D Human)

Expression

Mammalian

Available Since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pKANAC3K

Plasmid

#221545

Purpose

Activation tagging for plant

Depositor

Keiichi Shimizu

Type

Empty backbone

Use

Activation tagging in plant

Promoter

Cauliflower mosaic virus 35S

Available Since

Sept. 26, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pMSCV-FKBP-GMIP(pLxIS)

Plasmid

#221270

Purpose

Expression of GMIP pLxIS (1-84) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP pLxIS (1-84) (Gmip Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TC alphaDdCBE T2

Plasmid

#221991

Purpose

Expresses mitochondrial cytosine base editor arm in mammalian cells

Depositor

Stephen Ekker

Article

Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

Insert

COX8A MTS-3xFLAG-TALE NT-alphaN-TC TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

TALE: Q231R, W232G, S233A

Promoter

CMV

Available Since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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