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Plasmid#135812PurposeThis plasmid can be used to express independently eGFP and TagRFP from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertTagRFP
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(+6)
Plasmid#89728PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a longer version of the wt spacer (+6) extended by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a longer versi…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-6)
Plasmid#89730PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-6) truncated by 6 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-12)
Plasmid#89731PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-12) truncated by 12 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-18)
Plasmid#89732PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-18) truncated by 18 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDF-casBCDE(-3)
Plasmid#89729PurposeExpresses the Cascade subunits Cse2, Cas7, Cas5, Cas6e and pre-crRNA containing a shorter version of the wt spacer (-3) truncated by 3 nucleotides at the leader-distal end.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationDerivative CRISPR array containing a shorter vers…PromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
Antibody#213664-rAbPurposeAnti-Integrin αL (Human) chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds αL subunit.DepositorRecommended ApplicationsFlow CytometryReactivityHumanSource SpeciesRabbitIsotypeIgG1Trial SizeNot available to purchaseAvailable sinceMarch 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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Antibody#213662-rAbPurposeAnti-Integrin αL (Human) chimeric recombinant antibody with fused mouse variable and rabbit constant domains; binds αL subunit.DepositorRecommended ApplicationsFlow CytometryReactivityHumanSource SpeciesRabbitIsotypeIgG1Trial SizeNot available to purchaseAvailable sinceMarch 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits
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pCS2-Prom1-TEVp-TwinStrep-His / IRES2 / Pcdh21-TEVp-Myc-Flag
Plasmid#210820PurposeMammalian overexpression vector for polycistronic co-expression of C-terminally Strep and His tagged human Prom1s1 (WT) and C-terminally Myc and Flag tagged human Pchd21 (WT)DepositorUseTagsMyc, 3xFlag and TwinStrep, 10xHisExpressionMammalianMutationPromoterCMV and CMV / IRES2Available sinceJuly 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-pA-GI-wt1bDNNterm-E1b-5xUAS-E1b-eGFP-GI-pA-Tol2; gcryst:CFP
Plasmid#135766PurposeThis plasmid can be used to express independently eGFP and a zebrafish wt1b dominant negative isoform (N-terminal domain) from a bidirectional 5xUAS. Also CFP expression under crystallin promoter.DepositorInsertWilms Tumor 1b gene (N-terminal domain) (wt1b Zebrafish)
UseZebrafishTagsExpressionMutationPromoterUASAvailable sinceJan. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL7055
Plasmid#167224Purposeorthogonal T7 RNAP sfGFP reporterDepositorInsertpRV-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7057
Plasmid#167226Purposeorthogonal T7 RNAP sfGFP reporterDepositorInsertpIRH-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7058
Plasmid#167227Purposeoverexpression of orthogonal T7 RNAPDepositorInsertJ23119-AKSIRV
UseSynthetic BiologyTags6X HisExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7069
Plasmid#167238Purposemalachite green aptamer tagged sfGFPDepositorInsertJ23119-sfGFP-MGA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7070
Plasmid#167239Purposemalachite green aptamer tagged sfGFPDepositorInsertpT7-sfGFP-MGA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7090
Plasmid#167252Purposecell-free mercury sensor with enzymatic outputDepositorInsertpMer-XylE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7091
Plasmid#167253Purposecell-free arsenic sensor with enzymatic outputDepositorInsertpArs-XylE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7093
Plasmid#167254Purposecell-free lead sensor with XylE outputDepositorInsertpPbr-XylE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7042
Plasmid#167213Purposeoverexpression of MerR in E. coli for cell-free mercury sensingDepositorInsertMerR
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJBL7049
Plasmid#167219Purposecell-free arsenic sensing reporterDepositorInsertpArs-sfGFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only