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aTY178_pUAS_Dkk1-IRES-tagBFP2_WPRE

Plasmid

#225353

Purpose

Lentiviral vector for DKK1 and BFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

Dkk1 (Dkk1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 9, 2025

Availability

Academic Institutions and Nonprofits only

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aTY135_mtk02_pCAG-nfGFPtether_P2A_H2B-IRFP_SV40pA

Plasmid

#225350

Purpose

piggyback backbone for non-fluorescent GFP tether and H2B-IRFP expression

Depositor

Wendell Lim

Article

Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

Insert

H2B (H2B Synthetic, Human)

Tags

miRFP and non-fluorescent GFP (Y67F)-P2A

Expression

Mammalian

Available Since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pHIE323-LPIV Puro-P2A-miRFP720 ZF6x12-C-DsRed-Express2(x6) phEF1α-BlastR

Plasmid

#201539

Purpose

Landing pad integration vector used to generate ZF6 synTF reporter line (HIE156), assembled with pPD1178 (destination vector), pHIE324-329 (TUPV1-6), pHIE280 (TUPV7), pPD1157 (linker)

Depositor

Joshua Leonard

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Inserts

PuromycinR-P2A-miRFP720
ZF6x12-C-DsRed-Express2(x6)
BlasticidinR

Expression

Mammalian

Promoter

None, ZF6x12(C)_YB_TATA Minimal Promoter, and hEF…

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

Plasmid

#191572

Purpose

Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.

Depositor

Joshua Leonard

Article

Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10

Insert

SARS-CoV-2 Spike Protein (S Synthetic)

Tags

3x FLAG

Expression

Mammalian

Mutation

D614G and F486S (confers reduced binding affinity…

Promoter

CMV

Available Since

Aug. 9, 2024

Availability

Academic Institutions and Nonprofits only

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95.b_pSTC0-zeo

Plasmid

#202335

Purpose

Design 95.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

95.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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90.b_pSTC0-zeo

Plasmid

#202337

Purpose

Design 90.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

90.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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80.b_pSTC0-zeo

Plasmid

#202339

Purpose

Design 80.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

80.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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70.b_pSTC0-zeo

Plasmid

#202341

Purpose

Design 70.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

70.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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50.b_pSTC0-zeo

Plasmid

#202343

Purpose

Design 50.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

50.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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opt.a_pSTC0-zeo

Plasmid

#202347

Purpose

Design opt.a (greedy optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

opt.a

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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opt.b_pSTC0-zeo

Plasmid

#202348

Purpose

Design opt.b (parallel tempering optimization without distance constraints) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

opt.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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rw-consensus_pSTC0-zeo

Plasmid

#202349

Purpose

Control rw-consensus (reweighted consensus) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

rw-consensus

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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neg.ctrl_pSTC0-zeo

Plasmid

#202350

Purpose

Control neg. ctrl (negative control S70A) in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

neg. ctrl

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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98.b_pSTC0-zeo

Plasmid

#202333

Purpose

Design 98.b in modified pSTC0 vector in which kan resistance cassette is replaced with zeo resistance cassette

Depositor

Nicholas Gauthier

Article

Fram et al Nat Commun. 2024 Jun 20;15(1):5141. doi:

Insert

98.b

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-Uncx [N155/9R]

Plasmid

#114545

Purpose

Mammalian Expression Plasmid of anti-Uncx (Mouse). Derived from hybridoma N155/9.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Uncx (Mus musculus) recombinant mouse monoclonal antibody (Uncx Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pST_CAHS1_PARRC_150-189 (pBS0658)

Plasmid

#185201

Purpose

For the mammalian expression of the tardigrade protein CAHS1_PARRC_150-189. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

CAHS1_PARRC_150-189

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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PvLEA4_repeats_k3_26_mCh-SspB (pBS1075)

Plasmid

#185299

Purpose

Mammalian expression of sleeping chironomid protein PvLEA4_repeats_k3_26 attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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DHN_VrDHN1a_mCh-SspB (pBS1074)

Plasmid

#185298

Purpose

Mammalian expression of riverbank grape plant protein DHN_VrDHN1a attached to SspB for light inducible binding to iLID scaffolds. Systems like this can be used to induce condensate formation.

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

PvLEA4_repeats_k3_26

Expression

Mammalian

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)

Plasmid

#185314

Purpose

For the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

pvLEA22mer_shuffle_3

Expression

Mammalian

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pST_APOE_HUMAN_A234G (pBS0816)

Plasmid

#185267

Purpose

For the mammalian expression of the human protein APOE_HUMAN_A234G. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

APOE_HUMAN_A234G

Expression

Mammalian

Mutation

A234G

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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