We narrowed to 1,286 results for: post
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Plasmid#123179PurposeExpresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus2 (aa 158-238) (STAT3 Human)
TagsVenus2 (aa 158-238) for bimolecular fluorescence …ExpressionMammalianMutationDeletion of the SH2 and TAD domains (C-terminus, …Available SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
Venus1-DeltaCT-STAT3
Plasmid#123178PurposeExpresses a STAT3 deletion mutant (without the C-terminus from SH2 domain) fused to the 1-157 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with Venus1 (aa 1-157) (STAT3 Human)
TagsVenus1 (aa 1-158) for bimolecular fluorescence co…ExpressionMammalianMutationDeletion of the SH2 and TAD domains (C-terminus, …Available SinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-puro
Plasmid#167824PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
Tagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-blast
Plasmid#167825PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
Tagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-NEDD4L_W2-W3-W4-HECT (349)
Plasmid#187721PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (349) in insect cellsDepositorInsertNEDD4L residues 349-975 (NEDD4L Human)
TagsGST-HRV 3C siteExpressionInsectMutationdeletion of residues 1-348Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_C2-W1-L-W2-HECT
Plasmid#187723PurposeExpress His-tagged NEDD4L C2-W1-L-W2-HECT chimera in insect cellsDepositorInsertNEDD4L residues 1-418+582-975 (NEDD4L Human)
TagsHis-TEVExpressionInsectMutationdeletion of residues 419-581Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBacHTA-NEDD4L_W1-L-W2-HECT
Plasmid#187724PurposeExpress His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cellsDepositorInsertNEDD4L residues 153-418+582-975 (NEDD4L Human)
TagsHis-TEVExpressionInsectMutationdeletion of residues 1-152 and 419-581Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (381-975)_pcDNA3.1(+)
Plasmid#187725PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-380Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (492-975)_pcDNA3.1(+)
Plasmid#187726PurposeExpress his-tagged NEDD4L W3-W4-HECT in mammalian cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-491Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)
Plasmid#187727PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 226-382Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)
Plasmid#187728PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
TagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 418-495Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)
Plasmid#187708PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-380Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT (492)
Plasmid#187709PurposeExpress his-tagged NEDD4L W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT_C874only
Plasmid#187711PurposeExpress his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cellsDepositorInsertNEDD4L residues 492-975 with mutations (NEDD4L Human)
TagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491; mutations C702A, C776…Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_FL-CS
Plasmid#187713PurposeExpress the catalytically inactive GST-tagged NEDD4L FL in bacterial cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationCys874 mutated to a serineAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_Δ1,2-linker
Plasmid#187714PurposeExpress GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 226-382Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_Δ2,3-linker
Plasmid#187715PurposeExpress GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 418-495Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)
Plasmid#187716PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cellsDepositorInsertNEDD4L with residues XX (NEDD4L Human)
TagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-347Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K49R-STAT3
Plasmid#203721PurposeExpresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
TagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K49R substitutionsPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K685R-STAT3
Plasmid#203722PurposeExpresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
TagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K685R substitutionsPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only