We narrowed to 1,255 results for: post
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Plasmid#187724PurposeExpress His-tagged NEDD4L W1-L-W2-HECT chimera in inserct cellsDepositorInsertNEDD4L residues 153-418+582-975 (NEDD4L Human)
UseTagsHis-TEVExpressionInsectMutationdeletion of residues 1-152 and 419-581PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (381-975)_pcDNA3.1(+)
Plasmid#187725PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT (381) in mammalian cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-380PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L (492-975)_pcDNA3.1(+)
Plasmid#187726PurposeExpress his-tagged NEDD4L W3-W4-HECT in mammalian cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 1-491PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ1,2-linker_pcDNA3.1(+)
Plasmid#187727PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in mammalian cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 226-382PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
His-Precission-NEDD4L_Δ2,3-linker_pcDNA3.1(+)
Plasmid#187728PurposeExpress his-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in mammalian cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionMammalianMutationdeletion of residues 418-495PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)
Plasmid#187708PurposeExpress his-tagged NEDD4L W2-W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 381-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-380PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT (492)
Plasmid#187709PurposeExpress his-tagged NEDD4L W3-W4-HECT in bacterial cellsDepositorInsertNEDD4L residues 492-975 (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)-NEDD4L_W3-W4-HECT_C874only
Plasmid#187711PurposeExpress his-tagged NEDD4L W3-W4-HECT_C874only in bacterial cellsDepositorInsertNEDD4L residues 492-975 with mutations (NEDD4L Human)
UseTagsHis-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-491; mutations C702A, C776…PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_FL-CS
Plasmid#187713PurposeExpress the catalytically inactive GST-tagged NEDD4L FL in bacterial cellsDepositorInsertNEDD4L FL with C874 mutated to a serine (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationCys874 mutated to a serinePromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_Δ1,2-linker
Plasmid#187714PurposeExpress GST-tagged NEDD4L FL with removal of the linker between the WW1 and WW2 domains in bacterial cellsDepositorInsertNEDD4L with residues 226-382 removed (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 226-382PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_Δ2,3-linker
Plasmid#187715PurposeExpress GST-tagged NEDD4L FL with removal of the linker between the WW2 and WW3 domains in bacterial cellsDepositorInsertNEDD4L with residues 418-495 removed (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 418-495PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-NEDD4L_W2-W3-W4-HECT (348)
Plasmid#187716PurposeExpress GST-tagged NEDD4L W2-W3-W4-HECT (348) in bacterial cellsDepositorInsertNEDD4L with residues XX (NEDD4L Human)
UseTagsGST-HRV 3C siteExpressionBacterialMutationdeletion of residues 1-347PromoterAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K49R-STAT3
Plasmid#203721PurposeExpresses the PTM-resistant, double mutant Y705F+K49R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
UseTagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K49R substitutionsPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
VC-Y705F+K685R-STAT3
Plasmid#203722PurposeExpresses the PTM-resistant, double mutant Y705F+K685R - STAT3 fused to the 158-238 fragment of Venus in mammalian cellsDepositorInsertSTAT3 tagged with VC (aa 158-238) (STAT3 Human)
UseTagsVC (aa 158-238) for bimolecular fluorescence comp…ExpressionMammalianMutationY705F+K685R substitutionsPromoterCMVAvailable SinceDec. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTW5126
Plasmid#129732Purposepolyhistidine replacement of EatA passenger DUF corresponding to amino acids E541 -Q617 of the native EatA proteinDepositorInserteatA-DUF::His8
UseTags9His tagExpressionBacterialMutationreplaces the domain of unknown function correspon…PromoteraraBADAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
Aiolos in Artichoke
Plasmid#74452PurposeMonitor post-translational degradation of Aiolos (Lentiviral, SFFV.Aiolos-17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorInsertAiolos (IKZF3) (IKZF3 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterSFFV and EF1aAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5-ires-puro
Plasmid#167821PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5-ires-blast
Plasmid#167822PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN5
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4-ires-puro
Plasmid#167828PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN5(TA)-ires-puro
Plasmid#167823Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only