Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,236 results for: golden gate

Clear keywords
Showing: 5181 - 5200 of 7236 results

  1. pUC19mut_circIRES2_FLuc_3HA

    Plasmid
    #249685
    Purpose
    This plasmid is designed to evaluate the translation efficiency of IRES2, an internal ribosome entry site derived from the Potato virus Y ordinary strain (PVY-O).
    Depositor
    Timo Schlemmer
    Publication
    Cortot et al Bio Protoc. 2026 Aug 20;16(16):e5791. do
    Insert
    IRES of Potato virus Y
    Use
    Synthetic Biology
    Tags
    3xHA
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    July 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249685-plasmid-map-sequence-id-498353

    Enlarge

  2. pUC19mut_circIRES3_FLuc_3HA

    Plasmid
    #249686
    Purpose
    This plasmid is designed to evaluate the translation efficiency of IRES3, a internal ribosome entry site derived from the 5' UTR of Tobacco etch virus (TEV).
    Depositor
    Timo Schlemmer
    Publication
    Cortot et al Bio Protoc. 2026 Aug 20;16(16):e5791. do
    Insert
    IRES of Tobacco etch virus
    Use
    Synthetic Biology
    Tags
    3xHA
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    July 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249686-plasmid-map-sequence-id-498352

    Enlarge

  3. pUC19mut_circIRES4_FLuc_3HA

    Plasmid
    #249687
    Purpose
    This plasmid is designed to evaluate the translation efficiency of IRES4, an internal ribosome entry site derived from the Pelargonium flower break virus.
    Depositor
    Timo Schlemmer
    Publication
    Cortot et al Bio Protoc. 2026 Aug 20;16(16):e5791. do
    Insert
    IRES of Pelargonium flower break virus
    Use
    Synthetic Biology
    Tags
    3xHA
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    July 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249687-plasmid-map-sequence-id-498351

    Enlarge

  6. pUC19mut_circIRES9_FLuc_3HA

    Plasmid
    #249691
    Purpose
    This plasmid is designed to evaluate the translation efficiency of IRES9, an internal ribosome entry site derived from the AU-rich 5' UTR of the Turnip mosaic potyvirus (TuMV).
    Depositor
    Timo Schlemmer
    Publication
    Cortot et al Bio Protoc. 2026 Aug 20;16(16):e5791. do
    Insert
    IRES of Turnip mosaic potyvirus
    Use
    Synthetic Biology
    Tags
    3xHA
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    July 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249691-plasmid-map-sequence-id-498355

    Enlarge

  7. pUC19mut_circIRES10_FLuc_3HA

    Plasmid
    #249692
    Purpose
    This plasmid is designed to evaluate the translation efficiency of IRES10, an internal ribosome entry site derived from the Hibiscus chlorotic virus (HCRSV).
    Depositor
    Timo Schlemmer
    Publication
    Cortot et al Bio Protoc. 2026 Aug 20;16(16):e5791. do
    Insert
    IRES of Hibiscus chlorotic ringspot virus
    Use
    Synthetic Biology
    Tags
    3xHA
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    July 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249692-plasmid-map-sequence-id-498349

    Enlarge

  8. pUC19mut_circIRES11_FLuc_3HA

    Plasmid
    #249693
    Purpose
    This plasmid is designed to evaluate the translation efficiency of IRES11, an internal ribosome entry site derived from the 5' UTR of the maize HSP101 mRNA.
    Depositor
    Timo Schlemmer
    Publication
    Cortot et al Bio Protoc. 2026 Aug 20;16(16):e5791. do
    Insert
    IRES of Zea mays Hsp101 mRNA
    Use
    Synthetic Biology
    Tags
    3xHA
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available since
    July 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249693-plasmid-map-sequence-id-498350

    Enlarge

  10. pYSD087

    Plasmid
    #252977
    Purpose
    Encodes Myc-tagged S. cerevisiae Sed1p display anchor as a Type 4a part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Myc-tagged Sed1
    Expression
    Bacterial
    Available since
    July 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    252977-plasmid-map-sequence-id-514471

    Enlarge

  11. pYSD068

    Plasmid
    #252972
    Purpose
    Encodes Anti-HAtag scFv (Frankenbody) as a Type 3b' part to be used in the yeast secretion/display (YSD2.0) extension of the MoClo yeast toolkit (YTK)
    Depositor
    Paul Young
    Publication
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Anti-HA scFv Frankenbody
    Expression
    Bacterial
    Available since
    July 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    252972-plasmid-map-sequence-id-514468

    Enlarge

  12. pPD1431 HBS HIF1a feedback

    Plasmid
    #253899
    Purpose
    Integration Vector for LumiScarlet with the HBS(YB_TATA) promoter (TUPV1), mHIF1_* with the HBS(YB_TATA) promoter (TUPV2), and AkaLuc-P2A-mTagBFP2-P2A-BlastR with the CAG promoter (TUPV3)
    Depositor
    Joshua Leonard
    Publication
    Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1
    Insert
    LumiScarlet, mHIF1_*, AkaLuc, mTagBFP2 and BlastR
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    HBS(YB_TATA), HBS(YB_TATA), CAG
    Available since
    June 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253899-plasmid-map-sequence-id-514335

    Enlarge

  14. pPD1433 HBS with HIF1beta

    Plasmid
    #253901
    Purpose
    Integration Vector for LumiScarlet (TUPV1), mHIF1_* (TUPV2), and mHIF1_ (TUPV3) with HBS(YB_TATA) promoters, and AkaLuc-P2A-mTagBFP2-P2A-BlastR with the CAG promoter (TUPV4).
    Depositor
    Joshua Leonard
    Publication
    Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1
    Insert
    LumiScarlet, mHIF1_*, mHIF1_, AkaLuc, mTagBFP2 and BlastR
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    HBS(YB_TATA), HBS(YB_TATA), HBS(YB_TATA), CAG
    Available since
    June 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253901-plasmid-map-sequence-id-514333

    Enlarge

  15. pPD1403 Dox-Inducible LumiScarlet

    Plasmid
    #253892
    Purpose
    Integration Vector for LumiScarlet with the TRE3GV promoter (TUPV1), TetOn3G with the CAG promoter (TUPV2), and BlastR with the CAG promoter (TUPV3)
    Depositor
    Joshua Leonard
    Publication
    Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1
    Insert
    LumiScarlet, TetOn3G, and BlastR
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    TRE3GV, CAG, CAG
    Available since
    June 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253892-plasmid-map-sequence-id-514332

    Enlarge

  16. pPD1432 HBS HIF2a feedback

    Plasmid
    #253900
    Purpose
    Integration Vector for LumiScarlet with the HBS(YB_TATA) promoter (TUPV1), mHIF2_ with the HBS(YB_TATA) promoter (TUPV2), and AkaLuc-P2A-mTagBFP2-P2A-BlastR with the CAG promoter (TUPV3)
    Depositor
    Joshua Leonard
    Publication
    Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1
    Insert
    LumiScarlet, mHIF2_, AkaLuc, mTagBFP2 and BlastR
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    HBS(YB_TATA), HBS(YB_TATA), CAG
    Available since
    June 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253900-plasmid-map-sequence-id-514331

    Enlarge

  17. pPD1412 Simple CMV HBS

    Plasmid
    #253895
    Purpose
    Integration Vector for LumiScarlet with the HBS(CMV_min) promoter (TUPV1), and AkaLuc-P2A-mTagBFP2-P2A-BlastR with the CAG promoter (TUPV2)
    Depositor
    Joshua Leonard
    Publication
    Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1
    Insert
    LumiScarlet, AkaLuc-P2A-mTagBFP-P2A-BlastR
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    HBS(CMV_min), CAG
    Available since
    June 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253895-plasmid-map-sequence-id-514329

    Enlarge

  19. pPD790 HBS CMV_Min DsRE2 and Dox-HIF1a

    Plasmid
    #253879
    Purpose
    Integration Vector for DsRE2 reporter with the HBS (CMV_min) promoter (TUPV1), HIF1_* with the TRE3GV promtor (TUPV2), and EBFP2-P2A-BlastR with the CAG promoter (TUPV3)
    Depositor
    Joshua Leonard
    Publication
    Dreyer et al ACS Synth Biol. 2025 May 16;14(5):1464-1
    Insert
    DsRE2, HIF1_*, EBFP2-P2A-BlastR
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    HBS(CMV_min), TRE3GV, CAG
    Available since
    June 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    253879-plasmid-map-sequence-id-514326

    Enlarge

  20. pSR3036

    Plasmid
    #222629
    Purpose
    Sp-dCas9 CRISPRi plasmid targeting yejL (b2187) in E. coli MG1655
    Depositor
    Lauren Andrews
    Insert
    gRNA spacer sequence targeting yejL
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    PTac
    Available since
    June 24, 2026
    Availability
    Academic Institutions and Nonprofits only
    222629-plasmid-map-sequence-id-441106

    Enlarge

Showing: 5181 - 5200 of 7236 results