We narrowed to 22,823 results for: Sis

1. DOT1L-CC1

   Plasmid

   #111756

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   DOT1L (DOT1L Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   contains coiled coil #1 (residues 506-555)

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. DOT1L-CC2

   Plasmid

   #111755

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Article

   Zhang et al Genes Dev. 2018 Mar 1;32(5-6):341-346. d

   Insert

   DOT1L (DOT1L Human)
   

   Use

   Tags

   8xHis-Thrombin-SUMO-TEV

   Expression

   Bacterial

   Mutation

   Contains coiled coil #2 (residues 560-608)

   Promoter

   T7

   Available Since

   June 14, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pMCh-1540

   Plasmid

   #82433

   Purpose

   Plasmid for expression of GST-tagged human-Nematostella GW182 11W11A chimera in mammalian cells

   Depositor

   Marina Chekulaeva

   Article

   Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

   Insert

   hs-nvGW182 11W11A chimera
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

   Promoter

   EF1A

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-CARD14-L156P

   Plasmid

   #69918

   Purpose

   Expresses mutant FLAG-CARD14 in mammalian cells

   Depositor

   Francesca Capon

   Article

   Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

   Insert

   CARD14 isoform 3 (CARD14 Human)
   

   Use

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   L156P

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCJ190

   Plasmid

   #162667

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b(+)-Is-PETase-W159H-S238F

   Plasmid

   #112203

   Purpose

   pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12

   Depositor

   Gregg Beckham, Christopher Johnson

   Article

   Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115

   Insert

   PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
   

   Use

   Tags

   6XHIS

   Expression

   Bacterial

   Mutation

   W159H, S238F

   Promoter

   N/A

   Available Since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-CMV-Puro-PNPT1

   Plasmid

   #223315

   Purpose

   Lentiviral vector expressing human PNPT1

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Polyribonucleotide Nucleotidyltransferase 1 (PNPT1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCRISPR v2-sgPnpt1-2#

   Plasmid

   #234770

   Purpose

   knockout Pnpt1

   Depositor

   Judy Lieberman

   Article

   Miao et al Immunity. 2023 Nov 14;56(11):2523-2541.e

   Insert

   Pnpt1 (Pnpt1 )
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKT2/CAGXSP/CD63mScarlet

   Plasmid

   #182972

   Purpose

   Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

   Depositor

   Masamitsu Kanada

   Article

   Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

   Insert

   CD63-mScarlet (CD63 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available Since

   April 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCJ189

    Plasmid

    #162666

    Purpose

    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

    Depositor

    Gregg Beckham

    Article

    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

    Insert

    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by an 8 residue Gly/Ser linker
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    codon optimized for expression in E. coli K12

    Promoter

    T7/lac

    Available Since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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