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TRAV18

Plasmid

#237975

Purpose

Encodes TRAV18 allele, P2A, and TRBC to generate TCRs via cloning

Depositor

Michael Birnbaum

Article

Gaglione et al Immunity. 2026 Feb 10;59(2):477-493.e9.

Insert

TRBC-P2A-TRAV18 (TRAV18, TRBC1 Human)

Use

Synthetic Biology

Mutation

None

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m3

Plasmid

#204465

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127S mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR L125W-A127S mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m4

Plasmid

#204466

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR L125W-A127T mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m8

Plasmid

#204470

Purpose

This is a biosensor with decreased specificity to 3OC12-HSL and improved specificity to 3OHC14-HSL, containing LasR L128F-S129W mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR L128F-S129W mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-EGFP-FKBP12

Plasmid

#194453

Purpose

Mammalian expression vector that expresses the EGFP protein with FKBP12 fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FKBP12 (FKBP1A Human)

Tags

eGFP

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pMET7-EDHFR-FKBP12

Plasmid

#194454

Purpose

Mammalian expression vector that expresses the EDHFR protein with FKBP12 fused to it

Depositor

Sven Eyckerman

Article

Bogaert et al Methods Enzymol. 2023;684:253-287. doi:

Insert

FKBP12 (FKBP1A Human)

Tags

eDHFR

Expression

Mammalian

Promoter

SR alpha

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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PtetA with pBELO_L6-PT3-T4_CAMR_TAN+

Plasmid

#225644

Purpose

tetA promoter expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

Depositor

Andre Sanches Ribeiro

Article

Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

Insert

tetR/tetA

Tags

mCherry

Expression

Bacterial

Promoter

tetA promoter

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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MAC3-NTRK3

Plasmid

#222612

Purpose

Expression of MAC3-tagged NTRK3

Depositor

Markku Varjosalo

Article

Liu et al Methods Mol Biol. 2023;2690:281-297. doi

Insert

NTRK3 (NTRK3 Human)

Tags

MAC3

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Aug. 5, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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PTPI1-Dsred-URA3

Plasmid

#207017

Purpose

Expresses DsRed-Express 2 as a marker of cytosol, uses auxotrophic marker URA3(Saccharomyces cerevisiae).

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

DsRed-Express 2

Expression

Yeast

Promoter

pTPI1

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Pho8-406

Plasmid

#207006

Purpose

Integration of GFP-Pho8 as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Marker for yeast vacuolar membrane.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

PHO8

Tags

GFP

Expression

Yeast

Available Since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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AAV2_CAG_oROS-HT(C199S)_WPRE

Plasmid

#216415

Purpose

Encodes the loss-of-function mutation C199S of genetically encoded, chemigenetic fluorescent peroxide sensor oROS-HT in AAV viral vectors

Depositor

Andre Berndt

Article

Lee et al bioRxiv 2024.02.06.579232

Insert

oROS-HT(C199S)

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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PCUP1-GFP-Osh1-406

Plasmid

#207007

Purpose

Integration of Osh1-GFP as an additional copy in genome, expressed under CUP1 promoter. Uses auxotrophic marker URA3 (Saccharomyces cerevisiae) for selection. Yeast PMN pathway substrate.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

OSH1

Tags

GFP

Expression

Yeast

Available Since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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mutNLS V5-Twist

Plasmid

#201380

Purpose

Expresses Twist mutant for nucleocytoplasmic transport studies

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Twist (Twist1 Mouse)

Tags

V5

Expression

Mammalian

Mutation

K38R K73R

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-mini v2-HF24_F4

Plasmid

#195718

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#4 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F20

Plasmid

#195734

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#20 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pUC57-mini v2-HF24_F5

Plasmid

#195719

Purpose

This plasmid carries a fragment from a 24-fragment lacI/lacZ cassette split that was designed as a Golden Gate assembly with high fidelity.

Depositor

Eric Cantor

Article

Potapov et al ACS Synth Biol. 2018 Nov 16;7(11):2665-2

Insert

Fragment#5 of 24 fragment splits in lacI/lacZ cassette

Use

Synthetic Biology

Available Since

March 16, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCIneo-lambdaN-HA-HsDCP2_1-395_V

Plasmid

#147791

Purpose

Mammalian Expression of HsDCP2_1-395

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-395 (DCP2 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-withDmTD_H

Plasmid

#146506

Purpose

Mammalian Expression of HsDCP1a-withDmTD

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-withDmTD (DCP1A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a_539-582_G

Plasmid

#146412

Purpose

Mammalian Expression of HsDcp1a_539-582

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDcp1a_539-582 (DCP1A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-mut2_G

Plasmid

#146416

Purpose

Mammalian Expression of HsDCP1a-mut2

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-mut2 (DCP1A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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