We narrowed to 11,563 results for: Vars;

1. pSpCas9(BB)-2A-mCherry_MAPRE3-gRNA#3

   Plasmid

   #197423

   Purpose

   Knock out of EB3 in human cells by CRISPR/Cas9

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE3 gRNA #3 (targets Exon 1) (MAPRE3 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#1

   Plasmid

   #197424

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #1 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSpCas9(BB)-2A-GFP_MAPRE1-KI-gRNA#2

   Plasmid

   #197425

   Purpose

   π-element knock-in into exon 5 of MAPRE1

   Depositor

   Torsten Wittmann

   Article

   Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75

   Insert

   MAPRE1 gRNA #2 (targets Exon 5) for π-element knock-in (MAPRE1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT2/shCol1a1/Scarlet_Seq1.2

   Plasmid

   #201404

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pT2/shCol1a1/Scarlet-Seq1.1

   Plasmid

   #201401

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pT2/Col1a1/GFP4_Seq 1.1

   Plasmid

   #201400

   Purpose

   Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Article

   Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

   Insert

   COL1A1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-In18runon-PTPN22

   Plasmid

   #195377

   Purpose

   A human PTPN22 mutant (intron 18-runon) in Gateway pDONR221

   Depositor

   Patrick Concannon

   Article

   Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

   Insert

   PTPN22 (PTPN22 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Intron 18-runon PTPN22

   Promoter

   Available Since

   March 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-Myc-PSK2 (K57A)

   Plasmid

   #197118

   Purpose

   Expression of kinase-defective MYC-tagged PSK2 (K57A) / TAOK1 (K57A) in mammalian cells

   Depositor

   Jonathan Morris

   Article

   Zihni et al J Biol Chem. 2006 Mar 17;281(11):7317-23

   Insert

   TAOK1 (K57A) (TAOK1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Changed K 57 to A

   Promoter

   SV40

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. FLAG-In18runon-PTPN22

   Plasmid

   #195379

   Purpose

   Mammalian expression of a human PTPN22 mutant (intron 18-runon) with FLAG tag

   Depositor

   Patrick Concannon

   Article

   Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

   Insert

   PTPN22 (PTPN22 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Intron 18-runon PTPN22

   Promoter

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-Ex1-17-PTPN22

    Plasmid

    #195375

    Purpose

    Mammalian expression of a human PTPN22 mutant (exon 1-17) with FLAG tag

    Depositor

    Patrick Concannon

    Article

    Ge et al Diabetes. 2016 Mar;65(3):794-802. doi: 1

    Insert

    PTPN22 (PTPN22 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    PTPN22 mutant containing exon 1 to 17

    Promoter

    Available Since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGADT7-γ1

    Plasmid

    #197078

    Purpose

    Expression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Insert

    AP-1 γ1 (Ap1g1 Mouse)
    

    Use

    Tags

    GAL4 transcriptional activation domain (AD) fragm…

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. P2061

    Plasmid

    #186299

    Purpose

    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10

    Inserts

    LexA-HBD-B112
    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    2xLexop-minimalCyc1 and PACT1

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-U6-DR30(sapI)-CMV-intron-hfCas13d-pA

    Plasmid

    #195865

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CMV-intron-hfCas13d-pA

    Plasmid

    #195864

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CMV-intron-DIO-hfCas13d-pA

    Plasmid

    #195866

    Purpose

    To express hfCas13d

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    high fidelity (hf) version of CasRx (hfCas13d)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Yes: GTGGAATACATTACCAACGTGGTGTACGTG

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3_HTR2A-NTEV-TCS-GV-2xHA

    Plasmid

    #194366

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    HTR2A-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3_SP-AVPR2-NTEV-TCS-GV-2xHA

    Plasmid

    #194371

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3_SP-GLP1R-NTEV-TCS-GV-2xHA

    Plasmid

    #194379

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-GLP1R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3_SP-DRD1-NTEV-TCS-GV-2xHA

    Plasmid

    #194359

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    SP-DRD1-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3_DRD1-V2R-NTEV-TCS-GV-2xHA

    Plasmid

    #194360

    Purpose

    Split TEV assays

    Depositor

    Michael Wehr

    Article

    Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

    Insert

    DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)
    

    Use

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge