Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 7,198 results for: WUS

Showing: 5201 - 5220 of 7198 results

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pRetro-UMI

Plasmid

#222686

Purpose

CRISPR-screening

Depositor

Ulrich Elling

Article

Michlits et al Nat Methods. 2017 Dec;14(12):1191-1197.

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Mutation

PGK without BbsI cutsite

Available Since

Aug. 1, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2 EGFP sgRNA4

Plasmid

#164934

Purpose

Expresses EGFP sgRNA4 in mammalian cells

Depositor

Ji Luo

Article

Yuen et al Nucleic Acids Res. 2017 Nov 16;45(20):12

Insert

sgRNA4 targeting Enhanced green fluorescent protein (verified for knockout)

Use

Lentiviral

Promoter

EFS promoter

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAdEasy-hALDOA K230A

Plasmid

#210814

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Tags

HA

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pYPQ262F-ABE8e

Plasmid

#199179

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e-HF mediated A-G base editing

Depositor

Yiping Qi

Article

Sretenovic et al Plant Physiol. 2023 Aug 31;193(1):291-30

Insert

ABE8e(V106W)-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e(v106w)-zspcas9(d…

Tags

NLS

Expression

Plant

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mta1+exon minimal CMV pCDNA5

Plasmid

#212070

Purpose

LR vector for integration of Mta1+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mta1+exon(12nt) (Mta1 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gatad2a+exon minimal CMV pCDNA5

Plasmid

#212050

Purpose

LR vector for integration of Gatad2a+exon(75nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gatad2a+exon(75nt) (Gatad2a Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA-FLAG Chtop-exon minimal CMV pCDNA5

Plasmid

#212090

Purpose

LR vector for integration of Chtop-exon into N2a FRT rtTA3 expression cells (BioID vector)

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop-exon (Chtop Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Tcf12+exon minimal CMV pCDNA5

Plasmid

#212077

Purpose

LR vector for integration of Tcf12+exon(72nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Tcf12+exon(72nt) (Tcf12 Mouse)

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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3'-BirA*-FLAG minimal CMV pCDNA5

Plasmid

#212047

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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5'-BirA*-FLAG minimal CMV pCDNA5

Plasmid

#212046

Purpose

LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Camta2+exon minimal CMV pCDNA5

Plasmid

#212049

Purpose

LR vector for integration of Camta2+exon(21nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Camta2+exon(21nt) (Camta2 Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG L3mbtl3+exon minimal CMV pCDNA5

Plasmid

#212059

Purpose

LR vector for integration of L3mbtl3+exon(75nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

L3mbtl3+exon(75nt) (L3mbtl3 Mouse)

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Chtop+exon_siResist minimal CMV pCDNA5

Plasmid

#212083

Purpose

LR vector for integration of Chtop+exon(138nt)_siResist into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop+exon(138nt)_siResist (Chtop Mouse)

Expression

Mammalian

Mutation

GCCTATATGTCCAAGACCA

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Sap30bp+exon_siResist minimal CMV pCDNA5

Plasmid

#212085

Purpose

LR vector for integration of Sap30bp+exon(48nt)_siResist into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Sap30bp+exon(48nt)_siResist (Sap30bp Mouse)

Expression

Mammalian

Mutation

TCTAACCATCTGCAGGACA

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Chtop+exon minimal CMV pCDNA5

Plasmid

#212081

Purpose

LR vector for integration of Chtop+exon(138nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Chtop+exon(138nt) (Chtop Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Mef2a+exon minimal CMV pCDNA5

Plasmid

#212063

Purpose

LR vector for integration of Mef2a+exon(24nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Mef2a+exon(24nt) (Mef2a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kansl3+exon minimal CMV pCDNA5

Plasmid

#212053

Purpose

LR vector for integration of Kansl3+exon(78nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kansl3+exon(78nt) (Kansl3 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Gse1+exon minimal CMV pCDNA5

Plasmid

#212057

Purpose

LR vector for integration of Gse1+ex(108nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Gse1+ex(108nt) (Gse1 Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Kdm1a+exon minimal CMV pCDNA5

Plasmid

#212055

Purpose

LR vector for integration of Kdm1a+exon(12nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Kdm1a+exon(12nt) (Kdm1a Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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5'-3xFLAG Sap30bp+exon minimal CMV pCDNA5

Plasmid

#212073

Purpose

LR vector for integration of Sap30bp+exon(48nt) into N2a FRT rtTA3 expression cells

Depositor

Benjamin Blencowe

Article

Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

Insert

Sap30bp+exon(48nt) (Sap30bp Mouse)

Expression

Mammalian

Available Since

Jan. 31, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 5201 - 5220 of 7198 results

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