We narrowed to 18,046 results for: Mut

1. pCAG-i53-bpA-EF1BFP

   Plasmid

   #111145

   Purpose

   Expression of i53 and a BFP reporter in mammalian cells

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Inserts

   i53 (mutL Synthetic)
   BFP (BFP Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   human Ubiquitin, contains 8 point mutations

   Promoter

   CAG promoter and EF1

   Available Since

   April 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-AU1-mTOR-E2419K

   Plasmid

   #19994

   Depositor

   Fuyuhiko Tamanoi

   Article

   Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   mammalian target of rapamycin-E2419K (Mtor Rat)
   

   Use

   Tags

   AU1

   Expression

   Mammalian

   Mutation

   Changed Glutamine 2419 to Lysine

   Promoter

   Available Since

   Jan. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. shHIF2α(#2) (HIF2α-#2-pSUPER-retro-GFP/Neo)

   Plasmid

   #22131

   Depositor

   William Kaelin

   Article

   Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

   Insert

   synthethic oligonucleotide
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   synthethic oligonucleotide encoding shRNA targeti…

   Promoter

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. DD-DamV133A-LMNB1-IRES2-mCherry

   Plasmid

   #159599

   Purpose

   Shield-1 inducible transient mammalian expression of Dam-LMNB1 for DamID, containing the V133A mutant of Dam.

   Depositor

   Aaron Streets

   Article

   Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

   Insert

   DD-DamV133A-LMNB1-IRES2-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Dam V133A mutation

   Promoter

   CMV

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS416Gal TDP43 WT YFP

   Plasmid

   #27447

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pYPQ166

   Plasmid

   #109328

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

   Depositor

   Yiping Qi

   Article

   Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

   Insert

   zCas9
   

   Use

   CRISPR; Gateway compatible zcas9 entry clone

   Tags

   3x FLAG, NLS and NLS

   Expression

   Plant

   Mutation

   Zea mays codon-optimized Cas9

   Promoter

   Available Since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. wt dynamin 1 pEGFP

   Plasmid

   #34680

   Depositor

   Sandra Schmid

   Article

   Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

   Insert

   dynamin 1ba (DNM1L Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

8. μ2-HA-D176A

   Plasmid

   #32754

   Depositor

   Alexander Sorkin

   Article

   Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

   Insert

   μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D176A

   Promoter

   CMV

   Available Since

   Nov. 1, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pCAG-delta90GFP

   Plasmid

   #26645

   Depositor

   Anjen Chenn

   Article

   Wrobel et al Dev Biol. 2007 Sep 15. 309(2):285-97.

   Insert

   delta90 beta-catenin (Ctnnb1 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Deletion of first 90 aa of beta catenin

   Promoter

   Available Since

   Nov. 8, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAM233

    Plasmid

    #226655

    Purpose

    pcDNA3.1-p97-FLAG

    Depositor

    Andreas Martin

    Article

    Blythe et al Structure. 2019 Dec 3;27(12):1820-1829.e

    Insert

    p97 (VCP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV_JAK3_IRES_BFP

    Plasmid

    #206291

    Purpose

    Expresses the wild type form of human JAK3.

    Depositor

    Charles de Bock

    Article

    Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

    Insert

    JAK3 (JAK3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. DD-DamV133A-IRES2-mCherry

    Plasmid

    #159600

    Purpose

    Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.

    Depositor

    Aaron Streets

    Article

    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

    Insert

    DD-DamV133A-IRES2-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Dam V133A mutation

    Promoter

    CMV

    Available Since

    Sept. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS

    Plasmid

    #140008

    Purpose

    Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    4xPylT (Mma) cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    U25C

    Promoter

    h7SK

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ALK7 T194D

    Plasmid

    #80886

    Purpose

    mammalian expression of ALK7 T194D

    Depositor

    Aristidis Moustakas

    Article

    Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

    Insert

    ALK7 (Acvr1c Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T194D (constitutively active)

    Promoter

    CMV

    Available Since

    Aug. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCEP4-intron-BaLgp160

    Plasmid

    #100918

    Purpose

    Mammalian expression plasmid for Env from the BaL HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env with upstream intron
    

    Use

    Tags

    CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a-MBP-CBX1-eReader

    Plasmid

    #135089

    Purpose

    Bacterial expression of engineered CBX1 with enhanced binding to H3K9me

    Depositor

    Marcey Waters

    Article

    Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

    Insert

    CBX1 chromobox protein 1, engineered
    

    Use

    Tags

    6xhis-tag and MBP

    Expression

    Bacterial

    Mutation

    lysine 43 mutated to alanine; aspartic acid 59 mu…

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TR-Csy4-R115A/R119A

    Plasmid

    #80696

    Purpose

    Expresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.

    Depositor

    Aravind Asokan

    Article

    Borchardt et al RNA. 2015 Nov;21(11):1921-30. doi: 10.12

    Insert

    Csy4-R115A/R119A
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Arg115 and Arg119 both mutated to Ala

    Promoter

    CBA

    Available Since

    Aug. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXEE401B

    Plasmid

    #91716

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Donor-PIGA

    Plasmid

    #153530

    Purpose

    Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

    Depositor

    Hiroyuki Konishi

    Article

    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

    Insert

    PIGA (a 1,991-bp fragment from intron 5 and exon 6)
    

    Use

    Donor plasmid

    Tags

    N/A

    Expression

    Mutation

    No mutation

    Promoter

    No promoter

    Available Since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHDE-35S-Cas9-mCherry

    Plasmid

    #78931

    Purpose

    For CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plants

    Depositor

    Yunde Zhao

    Article

    Gao et al Plant Physiol. 2016 May 15. pii: pp.0066

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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