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M78 pIRESpuro-CBP-2xTEV-StrepTag

Plasmid

#17132

Depositor

Randall Moon

Publication

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Type

Empty backbone

Tags

2xTEV, CBP, and Streptavidin

Expression

Mammalian

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only

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M76 pIRESpuro-GLUE-hDsh2

Plasmid

#15101

Depositor

Randall Moon

Publication

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

Dsh2 (DVL2 Human)

Tags

CBP tag, HA, and streptag

Expression

Mammalian

Available since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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pAd-Track Flag GCN5 Y621A/P622A

Plasmid

#14425

Depositor

Pere Puigserver

Publication

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

GCN5 Y621A/P622A (KAT2A Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

Y621A, P622A. Catalytically inactive acetyltransf…

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles
KHBD00077

Plasmid

#34235

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG3827 (sc Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
SF-SON IRES Puro

Plasmid

#214402

Purpose

Expresses human SOX10, OLIG2, NKX6.2 and PAC (puromycin N-acetyl-transferase)

Depositor

Tanja Kuhlmann

Publication

Ehrlich et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

SOX10, Olig2, NKX6.2 (SOX10, OLIG2, NKX6-2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

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ALIX-mNeonGreen

Plasmid

#191191

Purpose

Full-length ALIX with C-terminal mNeonGreen tag.

Depositor

Jin Zhang

Publication

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (PDCD6IP Synthetic, Human)

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-pmEKAR4

Plasmid

#174440

Purpose

Plasma membrane-targeted EKAR4.

Depositor

Jin Zhang

Publication

Keyes et al Elife. 2020 May 26;9. pii: 57410. doi: 1

Insert

pmEKAR4

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_psychLight2

Plasmid

#163910

Purpose

Expresses psychLight2 in mammalian cells

Depositor

Lin Tian

Publication

Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

Insert

psychLight2

Tags

Flag tag

Expression

Mammalian

Promoter

CAG

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223 SARS-CoV-2 NSP2

Plasmid

#141256

Purpose

Gateway-compatible Entry vector, with insert of NSP2 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP2 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC1A5

Plasmid

#131974

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC1A5 (SLC1A5 Human)

Expression

Mammalian

Available since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pENTR4_G3BP1

Plasmid

#127104

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

G3BP1 (G3BP1 Human)

Use

Gateway Cloning

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDA in pMAX

Plasmid

#120402

Purpose

enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Publication

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

EDA-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pORTMAGE311B

Plasmid

#120418

Purpose

Expresses Lambda Beta and a dominant negative MutL E32K allele, controlled by XylS-Pm expression system for high precision and efficient MAGE experiments at 37°C. RSF1010 Ori; Broad host-range; KanR

Depositor

Csaba Pál

Publication

Szili et al Antimicrob Agents Chemother. 2019 Aug 23

Inserts

MutL E32K
Lambda Beta
XylS

Expression

Bacterial

Mutation

E32K mutation conferring dominant mutator phenoty…

Promoter

Pm

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pCDNA5FRT/TO_HA-mAID-nanobody

Plasmid

#117713

Purpose

Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

Depositor

Joerg Mansfeld

Publication

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

mAID-nanobody

Tags

triple HA

Expression

Mammalian

Mutation

codon optimized mAID for murine expression

Promoter

CMV

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCR8 JARID2

Plasmid

#114443

Purpose

Entry vector for gateway cloning containing human JARID2 coding sequence

Depositor

Adrian Bracken

Publication

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

JARID2 (JARID2 Human)

Use

Gateway entry cloning

Promoter

None

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET24a-VHH-std

Plasmid

#109417

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody. VHH-std also contains a T7, HA, BAP and His6 epitope

Depositor

Martin Spiess

Publication

Buser et al Proc Natl Acad Sci U S A. 2018 Jul 3;115

Insert

anti-GFP nanobody fused to a T7, HA, BAP and His6 epitope

Tags

BAP, HA, His6, and T7

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1

Plasmid

#86885

Purpose

C-terminal part of the split-BioID

Depositor

Mathieu Bollen

Publication

Munter et al FEBS Lett. 2016 Dec 29. doi: 10.1002/187

Insert

BirA* (AA141-321)- HA- NIPP1]

Tags

HA tag

Expression

Mammalian

Mutation

BirA Amino Acids 141-321

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Publication

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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CAG-DDdCas9VP192-T2A-EGFP-ires-puro

Plasmid

#69534

Purpose

DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

Depositor

Timo Otonkoski

Publication

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

DD-dCas9VP192-T2A-EGFP

Tags

C-term T2A-EGFP and DHFR Destabilised Domain

Expression

Mammalian

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGH00.0126

Plasmid

#64257

Purpose

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

Depositor

Mark Guiltinan, Siela Maximova

Publication

Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

Inserts

EGFP
neomycin phosphotransferase II

Use

Binary agrobacterium vector for plant transformat…

Expression

Plant

Promoter

E12-omega

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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