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We narrowed to 12,110 results for: NSI

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Showing: 10521 - 10560 of 12110 results

  1. roGFP_iE_C204S_pQE30

    Plasmid
    #48632
    Purpose
    Bacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)
    Depositor
    David Ron
    Article
    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.
    Insert
    roGFP1
    Tags
    HisTag
    Expression
    Bacterial
    Mutation
    insertion E147B, c204S
    Available Since
    Oct. 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    48632-plasmid-map-sequence-id-70381

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  2. mCherry_LD 0_CS A_tTA-BFP

    Plasmid
    #58873
    Purpose
    MESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Article
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    58873-plasmid-map-sequence-id-94234

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  3. pLaNGo11-3

    Plasmid
    #126054
    Purpose
    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell
    Depositor
    Ralf-Bernd Klösgen
    Article
    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs
    Inserts
    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x3 (5 aa linker)
    Use
    Binary
    Tags
    GFP1-10
    Expression
    Plant
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126054-plasmid-map-sequence-id-254921

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  11. pTKI-Tyr

    Plasmid
    #247647
    Purpose
    Expresses GFP-SUMO-Tyr-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Insert
    GFP-SUMO-Tyr-mCherry N-degron proteolysis dual-fluorescence reporter
    Use
    Mycobacterial integrating
    Tags
    HA tag and Myc tag
    Expression
    Bacterial
    Promoter
    PTet
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247647-plasmid-map-sequence-id-494850

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  12. pTKI-Ser

    Plasmid
    #247648
    Purpose
    Expresses GFP-SUMO-Ser-mCherry N-degron reporter driven by aTc responsive promoter, from KanR integrating mycobacterial plasmid lacking Ulp1
    Depositor
    Karl Schmitz
    Article
    Presloid et al Mol Microbiol. 2025 Jan;123(1):16-30. do
    Insert
    GFP-SUMO-Ser-mCherry N-degron proteolysis dual-fluorescence reporter
    Use
    Mycobacterial integrating
    Tags
    HA tag and Myc tag
    Expression
    Bacterial
    Promoter
    PTet
    Available Since
    Dec. 9, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    247648-plasmid-map-sequence-id-494849

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  17. pTARGEX-CHL

    Plasmid
    #242447
    Purpose
    Targets high levels of recombinant protein to chloroplast. Empty vector. Clone via SapI restriction site. mCherry dropout cassette will enable Red/White screening in E.coli.
    Depositor
    Allyson MacLean
    Article
    Chartrand-Pleau et al Front. Synth. Biol. 3:1619871
    Type
    Empty backbone
    Tags
    Transit peptide from N. tabacum RbcS
    Expression
    Plant
    Promoter
    double 35S
    Available Since
    Oct. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    242447-plasmid-map-sequence-id-490473

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  24. ACE2-Mutant-C4

    Plasmid
    #240608
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 4 located within the collectrin-like site on ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on ACE2-clus…
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    240608-plasmid-map-sequence-id-483854

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  26. ACE2-Mutant-C(3+4)

    Plasmid
    #240611
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 3 and Cluster 4 of ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on both ACE2…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240611-plasmid-map-sequence-id-482833

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  27. ACE2-Mutant-C(0+4)

    Plasmid
    #240610
    Purpose
    To test ACE2 cleavage by TMPRSS2. This protease targets R and K amino acids of ACE2, so these amino acids were substituted by A at Cluster 0 and Cluster 4 of ACE2
    Depositor
    Zaid Abassi
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    FLAG tag
    Expression
    Mammalian
    Mutation
    Arginine (R) and Lysine (K) residues on both ACE2…
    Promoter
    CMV
    Available Since
    Aug. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    240610-plasmid-map-sequence-id-482820

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  33. XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

    Plasmid
    #233382
    Purpose
    Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    VEGFR1(ecto+TM)-ADAR2dd
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233382-plasmid-map-sequence-id-466380

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  34. XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

    Plasmid
    #233386
    Purpose
    Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    IL2Rb(ecto+TM)-ADAR2dd
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233386-plasmid-map-sequence-id-466377

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  38. pMZ103-rbs1-m5

    Plasmid
    #204467
    Purpose
    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125F-A127G-S129N mutation.
    Depositor
    Lauren Andrews
    Article
    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58
    Inserts
    quorum sensing regulator LasR (lasR )
    eYFP
    Use
    Synthetic Biology
    Mutation
    LasR L125F-A127G-S129N mutation
    Promoter
    In an operon and PLas
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    204467-plasmid-map-sequence-id-403700

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  39. pMZ103-rbs1-m6

    Plasmid
    #204468
    Purpose
    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-S129N-L130V mutation.
    Depositor
    Lauren Andrews
    Article
    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58
    Inserts
    quorum sensing regulator LasR (lasR )
    eYFP
    Use
    Synthetic Biology
    Mutation
    LasR L125W-S129N-L130V mutation
    Promoter
    In an operon and PLas
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    204468-plasmid-map-sequence-id-413696

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  40. pMZ103-rbs1-m7

    Plasmid
    #204469
    Purpose
    This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L125W-A127T-L128M mutation.
    Depositor
    Lauren Andrews
    Article
    Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58
    Inserts
    quorum sensing regulator LasR (lasR )
    eYFP
    Use
    Synthetic Biology
    Mutation
    LasR L125W-A127T-L128M mutation
    Promoter
    In an operon and PLas
    Available Since
    March 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    204469-plasmid-map-sequence-id-403709

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Showing: 10521 - 10560 of 12110 results