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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 5281 - 5300 of 9221 results

  1. pGR595

    Plasmid
    #213785
    Purpose
    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.
    Depositor
    Chang Liu
    Insert
    BadBoy2 TP-DNAP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Available Since
    March 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    213785-plasmid-map-sequence-id-424581

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  2. pML104-HygMx4

    Plasmid
    #83475
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    HphMX4
    Expression
    Yeast
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83475-plasmid-map-sequence-id-358352

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  3. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  4. pML104-NatMx3

    Plasmid
    #83477
    Purpose
    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.
    Depositor
    Patricia Wittkopp
    Insert
    NatMx3
    Expression
    Yeast
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. AAV-DIO-TC66T-2A-oG

    Plasmid
    #178430
    Purpose
    Expresses TVA(TC66T) and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950 Glu66→Thr
    Optimized G
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Promoter
    hSyn
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178430-plasmid-map-sequence-id-354734

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  6. pBS-L1PA1CH blast

    Plasmid
    #69612
    Purpose
    Expresses a blasticidin tagged codon optimized L1PA1
    Depositor
    Astrid Roy-Engel
    Insert
    L1PA1
    Tags
    blast cassette
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69612-plasmid-map-sequence-id-123345

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  7. PB Neo* PB

    Plasmid
    #138413
    Purpose
    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Piggybac
    Expression
    Bacterial and Mammalian
    Available Since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138413-plasmid-map-sequence-id-316185

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  8. AAV-DIO-TVA-2A-oG

    Plasmid
    #178431
    Purpose
    Expresses TVA and optimized Rabies G (oG) protein in a FLEX cassette
    Depositor
    Edward Callaway
    Inserts
    TVA950
    Optimized G
    Use
    AAV and Cre/Lox; Adeno-associated virus
    Promoter
    hSyn
    Available Since
    Feb. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    178431-plasmid-map-sequence-id-354735

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  9. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187388-plasmid-map-sequence-id-370402

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  11. 6NM4

    Plasmid
    #162257
    Purpose
    Bacterial Expression
    Depositor
    Cheryl Arrowsmith
    Insert
    PRDM9 (PRDM9 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    162257-plasmid-map-sequence-id-319826

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  12. pCAS9i_TRP

    Plasmid
    #141253
    Purpose
    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.
    Depositor
    Adam Bertl
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    GAL1 (Cas9), pSNR52 (gRNA)
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    141253-plasmid-map-sequence-id-308890

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  15. pBCSK_HIS3_II

    Plasmid
    #109059
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109059-plasmid-map-sequence-id-209551

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  16. pBCSK_HIS3_I

    Plasmid
    #109058
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Expression
    Bacterial and Yeast
    Promoter
    HIS3
    Available Since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109058-plasmid-map-sequence-id-209548

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  18. pLV-Hygro-EF1A-hNUDT1/EGFP

    Plasmid
    #187035
    Purpose
    EGFP fused to the C-terminus of NUDT1 and a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Insert
    NUDT1 (NUDT1 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    187035-plasmid-map-sequence-id-372023

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  19. pLV-Hygro-EF1A-hNUDT1/3xFLAG

    Plasmid
    #187036
    Purpose
    A 3xFLAG tag fused to the C-terminus of NUDT1 and a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Insert
    NUDT1 (NUDT1 Human)
    Use
    Lentiviral
    Tags
    3XFLAG
    Expression
    Mammalian
    Promoter
    EF1A
    Available Since
    Oct. 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    187036-plasmid-map-sequence-id-372024

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  20. pMOD4-mT2A-mutated_rpsL-BSD-F

    Plasmid
    #182338
    Purpose
    Template plasmid for PCR amplification of initial recombineering cassette
    Depositor
    Mario Capecchi
    Insert
    BSD
    Use
    Mouse Targeting; Flp / frt
    Expression
    Bacterial
    Available Since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182338-plasmid-map-sequence-id-358191

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Showing: 5281 - 5300 of 9221 results