We narrowed to 33,720 results for: IND

1. Stx5L(m55V)-GFP

   Plasmid

   #209845

   Purpose

   Expresses Stx5 M55V mutant fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

   Insert

   STX5 (STX5 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Changed Methionine 55 to Valine, causes loss of S…

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Cav2.2 W391A pcDNA3

   Plasmid

   #206085

   Purpose

   expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

   Depositor

   Annette Dolphin

   Article

   Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

   Insert

   cacna1b (CACNA1B Rabbit)
   

   Expression

   Mammalian

   Mutation

   W391A

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mOrange2-NEDD1-gTBD

   Plasmid

   #196878

   Purpose

   Neuron-specific expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   mOrange2-N-gTBD
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Promoter

   Quimeric CAG

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-6P-1 AUP1 292-410 5E

   Plasmid

   #185332

   Purpose

   Bacterial expression of AUP1 with GST fusion protein for binding assays

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 292-410 only, with mutations R382E/Q383E/K390E…

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6P-1 AUP1 292-410 A397D

   Plasmid

   #185333

   Purpose

   Bacterial expression of AUP1 with GST fusion protein for binding assays

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   AUP1 (AUP1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 292-410 only, with mutation A379D

   Available Since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC1663 - pAAV SYN1 mGas6-Myc-DDK

   Plasmid

   #202540

   Purpose

   An adeno-associated viral vector expressing murine Gas6 fused Myc and DDK epitopes from a synapsin promoter

   Depositor

   Christopher Richie

   Article

   Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

   Insert

   Gas6 (Gas6 Mouse)
   

   Use

   AAV

   Tags

   Myc-DDK

   Promoter

   SYN1

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1-Gata6-3xT7

   Plasmid

   #200889

   Purpose

   Inducible lentiviral expression of Gata6

   Depositor

   Kai Ge

   Article

   Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

   Insert

   Gata6 (Gata6 Mouse)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   3xT7

   Expression

   Mammalian

   Promoter

   TRE promoter, Tet ON

   Available Since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-pSMANTIS_SMAD-FOS_Mutant

   Plasmid

   #194188

   Purpose

   Includes the promoter (1kb) of SMTS with mutated SMAD/FOS binding site

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Mutation

   mutated SMAD/FOS Site, Region 3-15nt of 1000nt

   Promoter

   SMANTIS promoter (1kb), mutated

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-pSMANTIS_STAT1-2_Mutant

   Plasmid

   #194189

   Purpose

   Includes the promoter (1kb) of SMTS with mutated STAT1/2 binding site

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Mutation

   mutated STAT1/2 site, Region 653-673nt of 1000nt

   Promoter

   SMANTIS promoter (1kb), mutated

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV_Syn_Xph18_eGFP_CCR5TC

    Plasmid

    #187443

    Purpose

    Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph18
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV_Syn_Xph15_eGFP_CCR5TC

    Plasmid

    #187442

    Purpose

    Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

    Depositor

    Matthieu Sainlos

    Article

    Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

    Insert

    Xph15
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHcRed-NPM1cOligodN243-C1

    Plasmid

    #131847

    Purpose

    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the oligo dimerization domain attached to the last 50 aminoacids in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a deletion contruct of the cytoplasmic m…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHcRed-NPM1cNLSdN243-C1

    Plasmid

    #131848

    Purpose

    Expresses HcRed-NPM1c (cytoplasmic mutant, human) with the NLS domain (aa. 121-195) attached to the last 50 aminoacids in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is a deletion contruct of the cytoplasmic m…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-B2

    Plasmid

    #165083

    Purpose

    gRNA 2 of pair B for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C1

    Plasmid

    #165085

    Purpose

    gRNA 1 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9n(BB)-2A-Puro (PX462) V2.0 Stx5DL-C2

    Plasmid

    #165086

    Purpose

    gRNA 2 of pair C for Stx5L-specific knockout

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    hSpCas9n-2A-Puro
    

    Use

    CRISPR

    Tags

    2A-Puro and 3XFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. KLC1N343S-Myc

    Plasmid

    #166963

    Purpose

    Expresses Myc-tagged KLC1 N343S protein in pcDNA3.1

    Depositor

    Rosalind Segal

    Article

    Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

    Insert

    KLC1-N343S (Klc1 Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    KLC1-N343S

    Promoter

    CMV

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. R619-M96-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(318-529)-3C-His8-SBP

    Plasmid

    #166020

    Purpose

    mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD) optimized for serology

    Depositor

    Dominic Esposito

    Article

    Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

    Insert

    SARS-CoV-2 RBD (S )
    

    Tags

    3C-His8-SBP

    Expression

    Mammalian

    Promoter

    CMV51

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

    Plasmid

    #153441

    Purpose

    E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

    Depositor

    Michael Nash

    Article

    Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

    Insert

    Rc. XDocB binding mode B knock-out mutant
    

    Tags

    3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

    Expression

    Bacterial

    Mutation

    Arginine 140 and methionine 144 were mutated to a…

    Promoter

    T7 promoter

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPN432

    Plasmid

    #137870

    Purpose

    Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

    Insert

    TCF4 (TCF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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