We narrowed to 41,904 results for: tro

1. pCRISPEY-Z3-Nat-ADE2

   Plasmid

   #232106

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPEY-GAL-Hyg-ADE2

   Plasmid

   #232104

   Purpose

   Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   GAL7

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPEY-Z3-Hyg-ADE2

   Plasmid

   #232107

   Purpose

   Expresses estradiol-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   ADE2 gRNA and repair template
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Repair template introduces C at nt 466 of ADE2 to…

   Promoter

   Z3

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. FUS-miRFP670-TRF1

   Plasmid

   #227378

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric FUS IDR, WT (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. iLID-miRFP670-TRF1

   Plasmid

   #227385

   Purpose

   Adhesion module, creates 'seed' at telomere

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric iLID (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRE3G-NS3/4A S139A

   Plasmid

   #186885

   Purpose

   Doxycycline-dependent expression of catalytically inactive NS3/4A protease gene (S140A) in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   HCV NS3/4A protease with S139A mutation
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   S139A

   Promoter

   TRE3G

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTRE3G-WHG cGAS-HA

   Plasmid

   #186867

   Purpose

   Doxycycline-dependent expression of white-handed gibbon cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   White-handed gibbon cGAS with C-terminal HA tag
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2

   Plasmid

   #221082

   Purpose

   Plasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.

   Depositor

   Mikhail Shapiro

   Article

   Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

   Insert

   ACC-msfGFP[r5M]_ACC-mEBFP2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   CMV, T7

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Zed_vector_tol2_+15.2prox1a:EGFP_XCA:DsRed2

   Plasmid

   #218206

   Purpose

   Zebrafish +15.2prox1a enhancer reporter in the FCLV from 3 dpf. XCA:DsRed2 as a control in the skeletal and cardiac muscle. Shows the high background typical of the ZED vector

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   +15.2prox1a
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHis-WzzE

    Plasmid

    #196661

    Purpose

    Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGST-WzzE

    Plasmid

    #196662

    Purpose

    Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    TEV-cleavable GST

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2.

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHis-mCherry-WzzE

    Plasmid

    #196663

    Purpose

    Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzzE
    

    Use

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    Valine insertion at position 2 of WzzE.

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWzyE-GST-distrepII

    Plasmid

    #196666

    Purpose

    Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    GST and TEV-cleavable di-strep tag II

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pWzyE-strep-mClover3

    Plasmid

    #196668

    Purpose

    Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

    Depositor

    James Naismith

    Article

    Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

    Insert

    WzyE
    

    Use

    Tags

    TEV-cleavable di-strep tag II and mClover3

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP4QE2Crimson

    Plasmid

    #218280

    Purpose

    Introduction of a bicistronic expression cassette of yEGFP and E2Crimson under the control of the SkGAL2 promoter

    Depositor

    Claudia Vickers

    Article

    Lu et al Nucleic Acids Res. 2024 May 29:gkae460.

    Insert

    pURA3>KlURA3>tKlURA3-pSkGAL2>yEGFP>ERBV1.2A>E2Crimson>tURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCF702

    Plasmid

    #209650

    Purpose

    Suicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicus

    Depositor

    Bastian Molitor

    Article

    Fink et al Appl Environ Microbiol. 2023 Jul 26;89(7

    Inserts

    Downstream homologous region
    thermostable neomycin phosphotransferase gene
    Upstream homologous region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMX-IG-ATG3 (H262A)

    Plasmid

    #212027

    Purpose

    Expresses untagged ATG3 (H262A) in mammalian cells

    Depositor

    Michael Lazarou

    Article

    Rao et al Sci Adv. 2024 Feb 9;10(6):eadj8027. doi:

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLINE1ΔORF2-scFvFc

    Plasmid

    #206021

    Purpose

    The ORF2 sequence was removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF2 sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLINE1ΔORF1-2-scFvFc

    Plasmid

    #206022

    Purpose

    The ORF1 and ORF2 sequences were removed from pLINE1-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, lacking the ORF1 and ORF2 sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pROF366

    Plasmid

    #155332

    Purpose

    Expression of FLuc under the control of AtAP1 promoter

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PAtAP1-FLuc-TSV40
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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