Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 18,525 results for: puro

Showing: 5321 - 5340 of 18525 results

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pKLV2-U6gRNA5(hIKBKG_1k)-PGKpuro2ABFP-W

Plasmid

#208410

Purpose

Lentiviral gRNA plasmid targeting human IKBKG gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

IKBKG (IKBKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hIKBKG_2m)-PGKpuro2ABFP-W

Plasmid

#208411

Purpose

Lentiviral gRNA plasmid targeting human IKBKG gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

IKBKG (IKBKG Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMAP3K7_1k)-PGKpuro2ABFP-W

Plasmid

#208412

Purpose

Lentiviral gRNA plasmid targeting human MAP3K7 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

MAP3K7 (MAP3K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hMAP3K7_2m)-PGKpuro2ABFP-W

Plasmid

#208413

Purpose

Lentiviral gRNA plasmid targeting human MAP3K7 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

MAP3K7 (MAP3K7 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(hRNF31_1k)-PGKpuro2ABFP-W

Plasmid

#208414

Purpose

Lentiviral gRNA plasmid targeting human RNF31 gene, co-expression of BFP tag

Depositor

Sok Ching Cheong

Article

Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

Insert

RNF31 (RNF31 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

N/A

Promoter

human U6

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N2 (1-399)

Plasmid

#225452

Purpose

Express mEGFP-fusion protein of FXR1 fragment (1-399)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N2 fragment (1-399)

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1-N1 (1-379)

Plasmid

#225450

Purpose

Express mEGFP-fusion protein of FXR1 fragment (1-379)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N1 fragment (1-379)

Promoter

CMV

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-N1(1-445)

Plasmid

#225464

Purpose

Express mEGFP-fusion protein of FMR1 fragment (1-445)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N1 fragment (1-445)

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-N2(1-491)

Plasmid

#225465

Purpose

Express mEGFP-fusion protein of FMR1 fragment (1-491)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

N2 fragment (1-491)

Promoter

CMV

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-Ccswap

Plasmid

#225460

Purpose

Express EGFP-fusion protein of FXR1 isoform a (CCswap)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CCswap (swapped positions of CC2 and CC1)

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-R rich

Plasmid

#225480

Purpose

Express mEGFP-GAPDH-Rrich fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with R-rich region of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-I304N

Plasmid

#225461

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (I304N)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

I304N

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor

Plasmid

#225479

Purpose

Express mEGFP-GAPDH-Tudor fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor domain of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-RGG

Plasmid

#225477

Purpose

Express mEGFP-GAPDH-RGG fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with RGG domain of TOP3B (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_NPM2-T2A-mGreenLantern-PuroTK

Plasmid

#222910

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to NPM2.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHDR_FIGLA-T2A-mGreenLantern-PuroTK

Plasmid

#222905

Purpose

Homology directed repair template for knocking in mGreenLantern reporter to FIGLA.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mGreenLantern

Use

CRISPR

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-TET2Puro-gal4DBD-miniVPR-HA

Plasmid

#207169

Purpose

Dox Inducible Expression Plasmid for gal4DBD-miniVPR-HA

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-miniVPR

Use

Lentiviral and Synthetic Biology

Tags

HA

Expression

Mammalian

Promoter

TRE3G

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-tet2puro-gal4DBD-HIFCAD

Plasmid

#207173

Purpose

Dox Inducible Expression Plasmid for gal4DBD-HIF-CAD

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Insert

Gal4DBD-HIF1a-CAD

Use

Lentiviral; Dox inducible

Tags

2x Myc

Expression

Mammalian

Promoter

TRE3G

Available Since

April 25, 2024

Availability

Academic Institutions and Nonprofits only

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p21(RxL)-mCerulean-PuroR

Plasmid

#215078

Purpose

Expresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

p21(RxL) denotes mutations: R19A, R20A, L21A, F22A

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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p21(dPCNA)-mCerulean-PuroR

Plasmid

#215079

Purpose

Expresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

CDKN1A (CDKN1A Human)

Use

Retroviral

Tags

mCerulean

Expression

Mammalian

Mutation

p21(dPCNA) denotes mutations M147A, D149A, F150A.

Promoter

LTR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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