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pTK_BsmBI_LacZ_Citrine_Nanotag_11

Plasmid

#130517

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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TU#1450_unc-53a_cDNA

Plasmid

#82361

Purpose

express unc-53a cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-53 a isoform (unc-53 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pJKW1847

Plasmid

#174004

Purpose

Expresses PpCYP94D108 and AtATR1 for reconstitution of diosgenin biosynthesis in yeast

Depositor

Jing-Ke Weng

Article

Christ et al Nat Commun. 2019 Jul 19;10(1):3206. doi:

Inserts

PpCYP94D108
ATR1 (ATR1 Mustard Weed)

Expression

Yeast

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pFL-TK-AID3'UTR

Plasmid

#19986

Depositor

Nina Papavasiliou

Article

Teng et al Immunity. 2008 May . 28(5):621-9.

Insert

AID 3' UTR (Aicda Mouse)

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pILGFPQ3

Plasmid

#83558

Purpose

Used to evaluate the expression output of GAL2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

GAL2 promoter-yEGFP

Expression

Yeast

Promoter

GAL2

Available Since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-DrU6a:sgRNA-Ds

Plasmid

#119069

Purpose

To clone sgRNA spacer sequence for microinjections in zebrafish

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

U6a:sgRNA

Expression

Bacterial

Promoter

Zebrafish U6a promoter

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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L1-D544N

Plasmid

#15499

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-D544N (L1CAM Human)

Expression

Bacterial

Mutation

Aspartic Acid 544 changed to Asparagine

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pre_IV_CS6_4323_v1

Plasmid

#126859

Purpose

4323 b scaffold from compatibility group 4, made by method IV, insert based on de Bruijn sequence with the an order of 7

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS6_4323_v1

Use

Synthetic Biology

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2-lyzC-microRNA 146a-Dendra2

Plasmid

#97113

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 146a Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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L1-S542P

Plasmid

#15489

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-S542P (L1CAM Human)

Expression

Bacterial

Mutation

Serine 542 changed to Proline

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
Tol2-lyzC-microRNA 199-3-Dendra2

Plasmid

#97119

Purpose

overexpression of microRNA and Dendra2 in Zebrafish

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

microRNA 199 3 Dendra2

Use

Zebrafish

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGGC001

Plasmid

#48824

Purpose

Provides WUS as GreenGate coding sequence module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

WUS (WUS Mustard Weed)

Use

Golden gate compatible cloning vector

Mutation

internal BsaI recognition site removed by silent …

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pJT3

Plasmid

#98304

Purpose

Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TRPL3AcNES1>TRPL41B

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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TU#1448_unc-73b_cDNA

Plasmid

#82362

Purpose

express unc-73b cDNA

Depositor

Martin Chalfie

Article

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 b isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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human pirh2-(138-189)

Plasmid

#24881

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains aa 138-189.

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only
L1-I219T

Plasmid

#15486

Depositor

Vance Lemmon

Article

Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

Insert

L1-I219T (L1CAM Human)

Expression

Bacterial

Mutation

Isoleucine 219 changed to Threonine

Available Since

Oct. 12, 2007

Availability

Academic Institutions and Nonprofits only
pMW96_6xHis-Sumo-TEV-mNG

Plasmid

#253320

Purpose

bacterial expression and purification of mNG

Depositor

Kristen Verhey

Insert

mNG

Tags

His-SUMO

Expression

Bacterial

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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hVEGF165-eGFP-hVEGF165 glycosylation sequon-His tag

Plasmid

#240484

Purpose

Express engineered glycosylated eGFP in CHO cells

Depositor

Xi Ren

Article

Xing et al Acta Biomater. 2025 Nov;207:221-232. doi

Insert

glycosylated eGFP

Tags

3 repeats of hVEGF165 glycosylation sequon, 6xHis…

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pDR-pBAD-ATG-6xHIS-AsiA

Plasmid

#245835

Purpose

Express anti-sigma protein, AsiA, in E. coli. His-Tag for Western Blots or Purification.

Depositor

Jie Xiao

Article

Weng et al Proc Natl Acad Sci U S A. 2019 Oct 1;116

Insert

AsiA

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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CIRC-V1-Tet-CVB3 IRES-EGFP-BbsI

Plasmid

#246756

Purpose

RNA circularization with CIRC using Tet intron, harbouring an CVB3 IRES-EGFP reporter.Plasmid was linearized with BbsI.

Depositor

Wensheng Wei

Article

Shen et al Nat Commun. 2025 Aug 10;16(1):7376. doi:

Insert

CIRC-V1-Tet-CVB3 IRES-EGFP-BbsI

Use

Synthetic Biology

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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