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pSB-CMV-MCS-Puro cPLA2-EGFP

Plasmid

#162568

Purpose

Used as a measure of cPLA2 activation in live cells with an EGFP tag.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Insert

cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95-mCherry

Plasmid

#125694

Purpose

Exogenous expression of PSD95 (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95-mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Synapsin

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMag(3x)-iRFP

Plasmid

#67305

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMag(3x)-iRFP (Tiam1 Mouse, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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EGFP-Bak

Plasmid

#32564

Depositor

Richard Youle

Article

Nechushtan et al J Cell Biol. 2001 Jun 11. 153(6):1265-76

Insert

human Bak (BAK1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#125692

Purpose

Global labeling of inhibitory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pFsynW SYP-GFP

Plasmid

#197283

Purpose

Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYP and GFP (Syp Rat)

Use

Lentiviral

Tags

1D4 epitope

Expression

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pWPT-PDX1

Plasmid

#12256

Depositor

Didier Trono

Article

Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

Insert

Pdx1 (PDX1 Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pSBbi_RP_lbMicroID2-HA

Plasmid

#188522

Purpose

Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

Depositor

James Londino

Article

Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

Insert

lbMicroID2

Use

Transposon mediated gene integration

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a/RPBSA

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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LentiGuide_CjCas9-Puro

Plasmid

#202564

Purpose

hU6-BsmBI-gRNA-Puro

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

CjCas9 sgRNA cassette

Use

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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piggybac-Syn-mScn8a_ICL1-sfGFP-P2A-mCherry

Plasmid

#182560

Purpose

Expresses Nav1.6 ICL1 tagged with sfGFP-P2A-mCherry

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

mouse Scn8a ICL1
sfGFP-P2A-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Syn

Available since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-TadCDd_gRNA

Plasmid

#202563

Purpose

P3-evoCjCas9-TadCDd and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-TadCDd

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSB1K3_M13ps_LacZα_gIII

Plasmid

#190435

Purpose

M13 packaging signal, LacZα gene, and M13 gIII gene (also called gp3, or g3p) cloned into the pSB1K3 vector.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

M13 packaging signal
M13 gIII
LacZα

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pUCBB-ctH6-eGFP

Plasmid

#32562

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

6 His tag with Thrombin site

Expression

Mutation

Promoter

Consitutive lac

Available since

Oct. 18, 2011

Availability

Academic Institutions and Nonprofits only

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AAV-P3-evoCjCas9-eAID_gRNA

Plasmid

#202562

Purpose

P3-evoCjCas9-eAID and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-eAID

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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Ii-Str_ManII-SBP-mCherry

Plasmid

#65256

Purpose

synchronize trafficking of ManII from the ER

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ii-Streptavidin and truncated Mannosidase II

Use

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYB2

Plasmid

#197282

Purpose

Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Q76V and F77W

Promoter

EF1alpha

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBT371

Plasmid

#125612

Purpose

Expresses CDA1-BE4max-NG in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

CDA1-BE4max-NG

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Tags

Expression

Mutation

Promoter

GAPDH

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-MEIOC

Plasmid

#222562

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of MEIOC

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

MEIOC (C17orf104 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pU6-p53 gRNA-Cas9-T2A-mCherry

Plasmid

#164256

Purpose

Transcription of p53 guide RNA and Cas9 expression for CRISPR/Cas9 in mammalian cells

Depositor

John Maciejowski

Article

Mohr et al Mol Cell. 2021 Jan 12. pii: S1097-2765(2

Insert

sg p53

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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