We narrowed to 2,134 results for: dcas9

1. lenti SYN-FLAG-dCas9-VPR

   Plasmid

   #114196

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SYN

   Available since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

   Plasmid

   #154139

   Purpose

   Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.

   Depositor

   Albert Jeltsch

   Article

   Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

   Insert

   dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP
   

   Use

   Tags

   3x FLAG and tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3XFLAG-VP64-SadCas9-NLS-VP64

   Plasmid

   #135338

   Purpose

   3xFLAG 2xVP64 SadCas9 for single guide cloning

   Depositor

   Ronald Cohn

   Article

   Kemaladewi et al Nature. 2019 Aug;572(7767):125-130. doi:

   Insert

   SadCas9
   

   Use

   Tags

   3xFLAG-VP64-SV40 NLS and NLS-VP64

   Expression

   Mammalian

   Mutation

   D10A and N580A

   Promoter

   CMV

   Available since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TRE-KRAB-dCas9-IRES-BFP

   Plasmid

   #85449

   Purpose

   Lentiviral vector expressing a KRAB-dCas9 fusion protein and BFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR_TRE3G-LSD1-dCas9-P2A-mCherry

   Plasmid

   #138462

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal LSD1-dCas9 fusion protein and mCherry

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   LSD1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TRE-KRAB-dCas9-IRES-GFP

   Plasmid

   #85556

   Purpose

   2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

   Depositor

   Eric Lander

   Article

   Fulco et al Science. 2016 Sep 29. pii: aag2445.

   Insert

   KRAB-dCas9-IRES-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA Tag, and KRAB domain

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid

    #192668

    Purpose

    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    TRE3G

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-TRE3G-dCas9-EGFP-HaloTag

    Plasmid

    #169451

    Purpose

    expression of dCas9-EGFP-HaloTag

    Depositor

    Stanley Qi

    Article

    Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

    Insert

    dCas9-EGFP-HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSH231-EF1-BLST-dCas9-VPR

    Plasmid

    #115148

    Purpose

    Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

    Depositor

    Michael Phelps, Raymond Monnat

    Article

    Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

    Insert

    BlastR-P2A-dCas9-VPR
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-TO-nls-st1dCas9-3nls-3XGFP

    Plasmid

    #64111

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. CMVp-dCas9-3xNLS-VP64 (Construct 1)

    Plasmid

    #55195

    Purpose

    Expresses taCas9 in Mammalian cells for transactivating endogenous and synthetic promoters. The backbone is a lentiviral vector.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/hUBC

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

    Plasmid

    #192664

    Purpose

    3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, D839A, H840A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCCL-PGK-SPdCas9-BFP-DNMT1

    Plasmid

    #66818

    Purpose

    dCas9 fused to BFP and the human DNMT1 catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dCas9-BFP-DNMT1, catalytic domain (DNMT1 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

    Plasmid

    #160626

    Purpose

    TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-dCas9:TV-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC18T-mini-Tn7T-hph-dCas9

    Plasmid

    #218371

    Purpose

    Expression of dCas9 for CRISPR-interference

    Depositor

    Michael Gebhardt

    Article

    Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PtetA

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. lenti-UCOE-dCas9-BFP-VP64

    Plasmid

    #196717

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting.

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-TagBFP-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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