We narrowed to 4,719 results for: pcr

1. pCR8-attL3-pegRNA-attL2

   Plasmid

   #213052

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-mScarlet-BlastR [M1G]

   Plasmid

   #171814

   Purpose

   Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mScarlet-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR8-attL1-pegRNA-attR3

   Plasmid

   #213051

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PCR4-Hsp68::eGFP-H11

   Plasmid

   #211941

   Purpose

   dual-enSERT-1 eGFP vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)

   Depositor

   Evgeny Kvon

   Article

   Hollingsworth et al Nat Commun. 2025 Jan 6;16(1):409. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSP68

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-AID-PcrA-UGI

   Plasmid

   #221220

   Purpose

   Express HACE Editor as a fusion of AID cytidine deaminase and PcrA helicase.

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Insert

   AID-PcrA-UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCR8-attL5-pegRNA-attR3

   Plasmid

   #213054

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-U6sgp53-U6sgbbSapI-GFAPCre

   Plasmid

   #100275

   Purpose

   AAV-CRISPR mediated genome editing in glia / astrocytes

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Type

   Empty backbone

   Use

   AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCR3-FLAG-TRAF6-C70A

   Plasmid

   #43926

   Depositor

   Bryant Darnay

   Article

   Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

   Insert

   TNF Receptor-Associated Factor 6 (Traf6 Mouse)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   C70A

   Promoter

   Available Since

   Jan. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCR8-attR4-pegRNA-attR3

   Plasmid

   #213056

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCR8-attL5-pegRNA-attL4

    Plasmid

    #213055

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCR8-attL1-pegRNA-attR5

    Plasmid

    #213053

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCRISPaint-2A-TurboGFP-PEST-PuroR

    Plasmid

    #80956

    Purpose

    CRISPaint gene tagging

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    TurboGFP-PEST
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPEY-GAL-Nat-ADE2

    Plasmid

    #232103

    Purpose

    Expresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

    Insert

    ADE2 gRNA and repair template
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Repair template introduces C at nt 466 of ADE2 to…

    Promoter

    GAL7

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-sfGFP-3xP3-RFP

    Plasmid

    #127566

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    sfGFP
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR3-FLAG-Ubc13-C87A

    Plasmid

    #43939

    Depositor

    Bryant Darnay

    Article

    Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

    Insert

    ubiquitin-conjugating enzyme E2N (UBE2N Human)
    

    Use

    Tags

    2x FLAG

    Expression

    Mammalian

    Mutation

    C87A

    Promoter

    Available Since

    Nov. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCR3-vsv-Sgo1 N61I

    Plasmid

    #108496

    Purpose

    expression of vsv-Sgo1 N61I (no PP2A binding)

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    Shugoshin 1 (SGO1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    no PP2A binding N61I

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS595 tet-hCMV-EGFPcre

    Plasmid

    #11957

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPaint-mNeon-PuroR [M1G]

    Plasmid

    #171800

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    Sept. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRII-1.1-2.01 beta

    Plasmid

    #69591

    Purpose

    OT-II TCR beta chain cDNA in pCRII

    Depositor

    Francis R. Carbone

    Article

    Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

    Insert

    TCR beta clone 1.1 (Tcrb Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPaint-mNeon-BlastR [M1G]

    Plasmid

    #171805

    Purpose

    Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mNeonGreen-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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