We narrowed to 4,668 results for: pcr

1. pAAV-U6sgp53-U6sgbbSapI-GFAPCre

   Plasmid

   #100275

   Purpose

   AAV-CRISPR mediated genome editing in glia / astrocytes

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Type

   Empty backbone

   Use

   AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPaint-sfGFP-3xP3-RFP

   Plasmid

   #127566

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes sfGFP-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   sfGFP
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCR3-FLAG-Ubc13-C87A

   Plasmid

   #43939

   Depositor

   Bryant Darnay

   Article

   Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

   Insert

   ubiquitin-conjugating enzyme E2N (UBE2N Human)
   

   Use

   Tags

   2x FLAG

   Expression

   Mammalian

   Mutation

   C87A

   Promoter

   Available since

   Nov. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPaint-2A-TurboGFP-PEST-PuroR

   Plasmid

   #80956

   Purpose

   CRISPaint gene tagging

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Insert

   TurboGFP-PEST
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPaint-mNeon-PuroR [M1G]

   Plasmid

   #171800

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS595 tet-hCMV-EGFPcre

   Plasmid

   #11957

   Depositor

   Brian Sauer

   Article

   Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

   Insert

   EGFP-cre (cre bacteriophage P1)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR-U6-gRNA-TRE3G-TdTomato

   Plasmid

   #192658

   Purpose

   A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, TRE3G

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PCR4-Hsp68::mCherry-H11

   Plasmid

   #211940

   Purpose

   dual-enSERT-1 mCherry vector for site-specific integration into the H11 locus (contains hsp68 minimal promoter)

   Depositor

   Evgeny Kvon

   Article

   Hollingsworth et al bioRxiv 2023.12.10.570890

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSP68

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-mNeon-BlastR [M1G]

   Plasmid

   #171805

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein and blasticidin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-T2A-BlastR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   BlastR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-mScarlet-BlastR [M1G]

    Plasmid

    #171814

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and blasticidin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-BlastR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    BlastR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVH607-Tier1-PCREm4-Nluc

    Plasmid

    #169594

    Purpose

    Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    cAMP response element-controlled NanoLuc Luciferase expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCREm4

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCri_L830-antiSH2-OptoMB-V416L

    Plasmid

    #159936

    Purpose

    Opto-MB bacterial expression

    Depositor

    Jared Toettcher, Jose Avalos

    Article

    Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

    Insert

    anti-SH2 MB with SS58 insert
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AsLOV2 V416L

    Promoter

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCri_L889-antiSH2-OptoMB-Triple

    Plasmid

    #159937

    Purpose

    Opto-MB bacterial expression

    Depositor

    Jared Toettcher, Jose Avalos

    Article

    Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

    Insert

    anti-SH2 MB with SS58 insert
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    AsLOV2 V416I_G528A_N538E

    Promoter

    Available since

    Nov. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL0812 (pCRISPR(BbsI)_Vch)

    Plasmid

    #200898

    Purpose

    Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    VchCRISPR (entry)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCR-BluntII-TOPO-RhoC

    Plasmid

    #14099

    Depositor

    Richard Hynes

    Article

    Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

    Insert

    Human RhoC (RHOC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pCR8-Magneto2.0-p2A-mCherry

    Plasmid

    #74334

    Purpose

    For gateway cloning Magneto2.0-p2A-mCherry into destination vector

    Depositor

    Ali Guler

    Article

    Wheeler et al Nat Neurosci. 2016 Mar 7. doi: 10.1038/n

    Insert

    Magneto2.0 (FTH1, FTL, Trpv4 Human, Rat)
    

    Use

    Gateway vector

    Tags

    Flag and p2A-mCherry

    Expression

    Mutation

    TRPV4: delta 760–871

    Promoter

    none

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCR-blunt-TOPO-FNDC5

    Plasmid

    #35970

    Depositor

    Bruce Spiegelman

    Article

    Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

    Insert

    fibronectin type III domain containing 5 (Fndc5 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 2, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pCRISPaint-mScarlet-PuroR [M1G]

    Plasmid

    #171809

    Purpose

    Universal donor plasmid for CRISPitope method encoding mScarlet fluorescent protein and puromycin resistance cassette [p.M1G]

    Depositor

    Michael Hoelzel

    Article

    Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

    Insert

    mScarlet-T2A-PuroR
    

    Use

    Gene tagging

    Tags

    Expression

    Mutation

    PuroR: Changed Methionin 1 to Glycine.

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-U6sgp53-mTSG-GFAPCre

    Plasmid

    #100276

    Purpose

    AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

    Insert

    GFAP promoter (Gfap, GFAP Mouse, Human)
    

    Use

    AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR-Blunt II-TOPO-PTPRD

    Plasmid

    #29705

    Depositor

    Todd Waldman

    Article

    Solomon et al Cancer Res. 2008 Dec 15. 68(24):10300-6.

    Insert

    Protein tyrosine phosphatase receptor-type delta (PTPRD Human)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    none

    Promoter

    Available since

    May 26, 2011

    Availability

    Academic Institutions and Nonprofits only