Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,839 results for: pcr

Clear keywords
Showing: 521 - 540 of 4839 results

  1. pCMV-TadA8e-PcrA M6

    Plasmid
    #221229
    Purpose
    Express HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.
    Depositor
    Fei Chen
    Publication
    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TadA8e-PcrA M6
    Expression
    Mammalian
    Available since
    Feb. 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    221229-plasmid-map-sequence-id-461180

    Enlarge

  2. pCRII-BbsI-sgRNAscaffold

    Plasmid
    #159352
    Purpose
    Expression vector for a customizable guide RNA
    Depositor
    Pim Pijnappel
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159352-plasmid-map-sequence-id-314976

    Enlarge

  5. pCMV-tadDE-PcrA M6-UGI

    Plasmid
    #221236
    Purpose
    Express HACE Editor as a fusion of the engineered dual base editor TadE that can perform citosine and adenine deamination and PcrA helicase.
    Depositor
    Fei Chen
    Publication
    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tadDE-PcrA M6-UGI
    Expression
    Mammalian
    Available since
    June 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    221236-plasmid-map-sequence-id-437117

    Enlarge

  12. pOpen-T4 g32

    Plasmid
    #165560
    Purpose
    A single-stranded DNA binding protein required for bacteriophage T4 replication and repair; Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure, to increase yield and processivity of reverse transcriptase during RT-PCR, and to increase yield and specificity of PCR products from soil samples.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    T4 gene 32
    Use
    Synthetic Biology
    Available since
    Aug. 16, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165560-plasmid-map-sequence-id-339953

    Enlarge

  13. pMaCTag-P19

    Plasmid
    #124789
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mCherry-mNeonGreen
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-mNeonGreen
    Use
    Pcr template
    Tags
    mCherry-mNeonGreen
    Promoter
    None
    Available since
    May 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    124789-plasmid-map-sequence-id-243814

    Enlarge

  14. pMaCTag-Z25

    Plasmid
    #120068
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with YFP-LID
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP-LID
    Use
    Pcr template
    Tags
    YFP-LID
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120068-plasmid-map-sequence-id-232677

    Enlarge

  19. pMaCTag-Z14

    Plasmid
    #120057
    Purpose
    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mNeptune2.5
    Depositor
    Michael Knop
    Publication
    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mNeptune2.5
    Use
    Pcr template
    Tags
    mNeptune2.5
    Promoter
    None
    Available since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    120057-plasmid-map-sequence-id-232666

    Enlarge

Showing: 521 - 540 of 4839 results