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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,479 results for: puc19

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Showing: 521 - 540 of 2479 results

  1. pTUB:FLuc

    Plasmid
    #114578
    Purpose
    Constitutive expression of firefly luciferase in T. gondii using the alpha tubulin promoter; floxed pyrimethamine selectable marker; UPRT locus flanking sequence to direct integration
    Depositor
    David Sibley
    Insert
    Firefly Luciferase
    Use
    Cre/Lox and Luciferase
    Promoter
    alpha tubulin T. gondii
    Available Since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    114578-plasmid-map-sequence-id-220131

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  3. pNR2E3-donor

    Plasmid
    #176369
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    NR2E3 (NR2E3 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176369-plasmid-map-sequence-id-347885

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  4. pRORB-donor

    Plasmid
    #176354
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    RORB (RORB Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176354-plasmid-map-sequence-id-347892

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  5. pZBED9-donor

    Plasmid
    #176344
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZBED9 (ZBED9 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    176344-plasmid-map-sequence-id-347889

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  7. pZNF22-donor

    Plasmid
    #176349
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    ZNF22 (ZNF22 Human)
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    176349-plasmid-map-sequence-id-347263

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  8. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

    Plasmid
    #169359
    Purpose
    C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    3XHA
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169359-plasmid-map-sequence-id-333203

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  9. pAW8-aid-V5-spo5DSR-Turg1:kanMX6

    Plasmid
    #169361
    Purpose
    C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    1XV5
    Expression
    Yeast
    Available Since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    169361-plasmid-map-sequence-id-333201

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  10. pAW8-aid-5Myc-spo5DSR-Turg1:kanMX6

    Plasmid
    #169360
    Purpose
    C-terminal aid-5Myc-spo5DSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Tags
    5XMyc
    Expression
    Yeast
    Available Since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169360-plasmid-map-sequence-id-332955

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  11. pZNF302-donor

    Plasmid
    #132513
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF302 (ZNF302 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    132513-plasmid-map-sequence-id-275216

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  12. pTSHZ1-donor

    Plasmid
    #132479
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    TSHZ1 (TSHZ1 Human)
    Use
    CRISPR
    Tags
    GFP
    Mutation
    none
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    132479-plasmid-map-sequence-id-274870

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  13. AbVec2.0-mIghg1

    Plasmid
    #127158
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg1 (Ighg1 Mouse)
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127158-plasmid-map-sequence-id-256842

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  14. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

    Plasmid
    #219826
    Purpose
    Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.
    Depositor
    Philipp Maass
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 29, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    219826-plasmid-map-sequence-id-440435

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  15. AbVec2.0-mIghg2c

    Plasmid
    #127159
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg2c (Ighg2c Mouse)
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127159-plasmid-map-sequence-id-256903

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  17. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Expression
    Bacterial
    Available Since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

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  18. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

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  19. pUPRT::Floxed DHFR-TS*

    Plasmid
    #100606
    Purpose
    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.
    Depositor
    David Sibley
    Insert
    TgUPRT::Floxed TgDHFR-TS*
    Use
    Toxoplasma expression
    Mutation
    Pyrimethamine resistance
    Promoter
    pTgDHFR-TS
    Available Since
    Sept. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    100606-plasmid-map-sequence-id-192862

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Showing: 521 - 540 of 2479 results