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tet_pLKO.1_puro_shNRF2 #1

Plasmid

#136584

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v1 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-HDR-mEGFP-Actin

Plasmid

#119870

Purpose

AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse Beta Actin

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Insert

beta Actin (Actb Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-AHPH-DM

Plasmid

#71368

Purpose

Location sensor for RhoA-GTP

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

C terminus of Anillin (ANLN Human)

Tags

EGFP

Expression

Mammalian

Mutation

A740D and E758K

Promoter

CMV

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pSIN4-EF1a-GATA2-IRES-Puro

Plasmid

#61063

Purpose

lentiviral vector for GATA2

Depositor

Igor Slukvin

Article

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

GATA2 (GATA2 Human)

Use

Lentiviral

Tags

HA

Promoter

EF1alpha

Available Since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

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AAV-SYP1-miniSOG-T2A-mCherry

Plasmid

#50972

Purpose

AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

Depositor

Roger Tsien

Article

Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

Has Service

AAV8

Insert

Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)

Use

AAV

Tags

T2A-mCherry and miniSOG

Promoter

human synapsin promoter

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37082

Purpose

Cre-dependent expression of hChR2(H134R) for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Promoter

EF1a

Available Since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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ΔNp63gamma-FLAG

Plasmid

#27012

Depositor

David Sidransky

Insert

ΔNp63gamma (TP63 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 15, 2010

Availability

Academic Institutions and Nonprofits only

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pGL3B-1081

Plasmid

#18759

Depositor

Clark Distelhorst

Article

Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

Insert

thioredoxin-interacting protein promoter (Txnip Mouse)

Use

Luciferase

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only
V250 pCEP-4HA B56epsilon

Plasmid

#14537

Depositor

David Virshup

Article

Seeling et al Science. 1999 Mar 26. 283(5410):2089-91.

Insert

PPP2R5C B56 epsilon regulatory subunit of PP2A (PPP2R5E Human)

Tags

4 HA

Expression

Mammalian

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only
Venus-GalT

Plasmid

#11931

Depositor

Jennifer Lippincott-Schwartz

Article

Cole et al Science. 1996 Aug 9. 273(5276):797-801.

Insert

GalT (GALT Human)

Expression

Mammalian

Mutation

Amino acids 1-60 of the human galactosyltransfera…

Available Since

Sept. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pARF6-YFP

Plasmid

#11389

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 6 (ARF6 Human)

Tags

YFP

Expression

Mammalian

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pYES2-HTH

Plasmid

#86470

Purpose

Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter

Depositor

Akash Gunjan

Article

Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

Type

Empty backbone

Tags

HIS10-TEV-HA

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 27, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pFastBac LIC cloning vector (4A)

Plasmid

#30111

Depositor

Scott Gradia

Type

Empty backbone

Expression

Insect

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pZS*12-MS2-GFP

Plasmid

#26245

Depositor

Philippe Cluzel

Article

Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

Insert

MS2-GFP

Expression

Bacterial

Mutation

five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

Available Since

Nov. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA3 CDK9 (D167N) HA (P#811)

Plasmid

#14641

Depositor

Matija Peterlin

Article

Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

Insert

cdk9 D167N (CDK9 Human)

Tags

HA

Expression

Mammalian

Mutation

D167N. Kinase negative mutant. Please see deposit…

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgRosa26

Plasmid

#159914

Purpose

Mutagenesis of Rosa26 with SauCas9

Depositor

Larry Zweifel

Insert

Rosa26 gRNA (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Actb KI

Plasmid

#131479

Purpose

Endogenous tagging of βactin: N-terminal (amino acid position: D2)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Actb, Actb Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-Exo84

Plasmid

#53762

Purpose

pEGFP-C3 plasmid that expresses Exo84

Depositor

Channing Der

Article

Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

Insert

Exo84 (EXOC8 Human)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD/HisB-iRFP720

Plasmid

#45460

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP720

Expression

Bacterial

Mutation

T2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…

Promoter

araBAD

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX-NICD

Plasmid

#44471

Depositor

Ernesto Canalis

Article

Zanotti et al J Biol Chem. 2011 Feb 11;286(6):4576-88.

Insert

Notch intracellular domain (Notch1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Contains amino acid 1753 to C-Term

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only