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pGEL678

Plasmid

#207511

Purpose

GmUbi3-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmUBI3

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL679

Plasmid

#207512

Purpose

GmM4-SpRY-STU

Depositor

Yong Zhang

Article

Chen et al Hortic Res. 2024 Jun 7;11(8):uhae160. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Plant

Promoter

GmM4

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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p27.pA.NGFR.mCMV.hPGK.Rab35(S22N).wpre[sequenced]

Plasmid

#226766

Purpose

Rab35(S22N)

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

rab35-mutant S22N (RAB35 )

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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p25.pA.NGFR.mCMV.hPGK.Rab35(WT).wpre[sequenced]

Plasmid

#226764

Purpose

rab35 (WT)

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

rab35 (RAB35 )

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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p26.pA.NGFR.mCMV.hPGK.Rab35(Q67L).wpre[sequenced]

Plasmid

#226765

Purpose

Rab35(Q67L)

Depositor

Ferdinando Pucci

Article

Ziglari et al Cancer Res. 2025 Mar 3;85(5):859-874. do

Insert

rab35-mutant Q67L (RAB35 )

Expression

Mammalian

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC10_pPromALB_STARRseq

Plasmid

#220289

Purpose

STARR-seq plasmid driven by minimal mouse Alb promoter; used to measure enhancer activity of a library of DNA sequences on interest. Useful for STARR-seq analysis in liver.

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGYL18_pPromALB_enhALB_RenillaLuc

Plasmid

#220284

Purpose

Renilla luciferase with minimal Albumin promoter with a 380 nt, -10.5 kb mouse Alb enhancer cloned into KpnI/XhoI-digested pGL4.70 (Promega)

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

Alb Enhancer

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTBY1-T4gp61/41 fusion

Plasmid

#228210

Purpose

Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

Depositor

Stephen Benkovic

Article

Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

Insert

61/41

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pRR5

Plasmid

#225095

Purpose

parental riboswitch reporter vector for regulation of the gfpUV fluorescent protein

Depositor

Robert Batey

Article

Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Promoter

synthetic

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pP4-A

Plasmid

#225096

Purpose

gfpUV reporter vector with a high performance adenine-dependent riboswitch

Depositor

Robert Batey

Article

Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

Insert

synthetic riboswitch

Expression

Bacterial

Promoter

synthetic

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pEXP(FLAG/HA-NSP1-WT)

Plasmid

#188781

Purpose

Expresses FLAG/HA-NSP1 in mammalian cells (SARS-CoV-2 NSP1)

Depositor

Markus Hafner

Article

Anastasakis et al PLoS One. 2024 Jan 26;19(1):e0297262. do

Insert

FLAG/HA-NSP1 (SARS-CoV-2)

Use

Frt site to insert into hek293 t-rex genome

Tags

FLAG/HA

Expression

Mammalian

Promoter

CMV Dox inducible

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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RT-2

Plasmid

#220438

Purpose

Base construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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plenti hSyn AldoC

Plasmid

#220917

Purpose

Neuronal specific expression of AldoC

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

AldoC (ALDOC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

hSyn

Available Since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pHB1

Plasmid

#204668

Purpose

pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs

Depositor

Mark Mandel

Article

Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do

Insert

FRT-erm-FRT

Use

Synthetic Biology

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAC13

Plasmid

#197407

Purpose

Honey bee gut symbiont broad-host range vector with constitutive GFP, ampR and pVS1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pVS1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC23

Plasmid

#197409

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and pBBR1 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pBBR1 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC24

Plasmid

#197410

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and pTFC-FC2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

pTF-FC2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAC25

Plasmid

#197411

Purpose

Honey bee gut symbiont broad-host range vector with constitutive E2-crimson, ampR and RK2 replicon

Depositor

Yolanda Schaerli

Article

Chhun et al PLoS Biol. 2024 Mar 5;22(3):e3002523. do

Insert

RK2 replicon

Use

Synthetic Biology

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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