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pMpGWB221

Plasmid

#68612

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-deltaY20

Plasmid

#58459

Purpose

Expresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 deleted

Promoter

CMV IE

Available Since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pMpGWB319

Plasmid

#68647

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Modified EAR motif plant-specific repression doma…

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFLAG-GATA2-D377-415

Plasmid

#1421

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 377 to 415

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pFLAG-GATA2-D415-475

Plasmid

#1422

Depositor

Gokhan Hotamisligil

Article

Tong et al Mol Cell Biol 2005 Jan;25(2):706-15.

Insert

GATA-2 (GATA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

deleted amino acids 414 to 475

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pLKO-sh-Myocardin

Plasmid

#100769

Purpose

Lentiviral expression of shRNA targeting MYOCD

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-sh-Myocardin

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-mIFITM3-Y20A

Plasmid

#58460

Purpose

Expresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (Ifitm3 Mouse)

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 to alanine

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
pFAB3913

Plasmid

#47816

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD9 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 14 and BCD9

Use

Synthetic Biology

Expression

Bacterial

Promoter

see insert

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_3

Plasmid

#73535

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pGL2 p15 4xSBR1 MutSBE

Plasmid

#15718

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR1 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutant SBE (Smad binding element)

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL3

Plasmid

#67320

Purpose

hArl3 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Use

Gateway entry vector

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
L1

Plasmid

#21200

Depositor

Paul Khavari

Article

Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

Insert

Mek2 (Esr1, MAP2K2 Human, Mouse)

Use

Retroviral

Mutation

Mek2 K101A fused to ER*, catalytically inactive

Available Since

July 24, 2009

Availability

Academic Institutions and Nonprofits only
psiCheck-ApoE3'UTR

Plasmid

#41846

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

ApoE 3'UTR (APOE Human)

Use

Luciferase

Available Since

Jan. 25, 2013

Availability

Academic Institutions and Nonprofits only
pMXs-ERas CVLS-IP

Plasmid

#13828

Depositor

Shinya Yamanaka

Article

Takahashi et al J Biol Chem. 2005 Sep 23. 280(38):32768-

Insert

ERas CVLS (Eras Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

CSVA (224-227) changed to CVLS (CAAX sequence of …

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC

Plasmid

#67687

Purpose

UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassette

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3796-38

Insert

dusp6 (dusp6 Zebrafish)

Tags

6xmyc-NLS-tdTomato-2A

Mutation

S159A, S196A

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G13

Plasmid

#25741

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 13 miR-shRNA

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pAG179 BC072452 3'UTR mut

Plasmid

#12037

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N12 3'UTR mut (FAM107B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
mCardinal-Myotilin-C-14

Plasmid

#56169

Purpose

Localization: Cytoskeleton, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

Myotilin (MYOT Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pWZLneo Flag hDPY30

Plasmid

#15555

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pOPTXGARE

Plasmid

#68542

Purpose

cyclic nucleotide reporter for bacteria or plant cells

Depositor

Helen Irving

Article

Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

Insert

OPTX promoter with GARE (AT1G33440 Mustard Weed)

Tags

luciferase

Expression

Bacterial and Plant

Mutation

5x GARE inserted 190 bp 5' pf ATG (GARE = ta…

Promoter

OPTX promoter with GARE

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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