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pJZC35

Plasmid

#62325

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pTetR-BCD7-phlF-ssrA P66U18C51T15 v40-3a (492)

Plasmid

#70740

Purpose

plasmid for building 3n2, 5n1, 5n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

phlF-ssrA

Available Since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pPhlF-BCD20-Citrine-ssrA P64U23C88T18 v1-1 (586)

Plasmid

#70743

Purpose

for building 3-color reporter plasmid

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

Citrine-ssrA

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSrpR-BCD7-tetR-ssrA P65U18C45T14 v40-2 (491)

Plasmid

#70736

Purpose

plasmid for building 3n2

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

tetR-ssrA

Available Since

Feb. 3, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_PEST2D

Plasmid

#62434

Purpose

MXS_chaining vector with PEST2D

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Destabilization tag

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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PP161

Plasmid

#78993

Purpose

Supplemental Figure 4. Promoter GAP3. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PP164

Plasmid

#78988

Purpose

Supplemental Figure 4. Promoter short ppTEF1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP149

Plasmid

#78989

Purpose

Supplemental Figure 4. Promoter WT GAP. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP154

Plasmid

#78991

Purpose

Supplemental Figure 4. Promoter GAP1. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP162

Plasmid

#78995

Purpose

Supplemental Figure 4. Promoter GAP5. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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PP246

Plasmid

#78966

Purpose

Promoter: GAP6, minimal Promoter: AOX1. Figure 4. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Expression

Yeast

Available Since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC102

Plasmid

#62337

Purpose

sgRNA for mammalian cells with mCherry marker

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 13, 2015

Availability

Academic Institutions and Nonprofits only

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pMPO 57

Plasmid

#68361

Purpose

Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF attenuator-MCSII, ColE1 replication origin

Depositor

Eduardo Santero

Article

Medina et al PLoS One. 2011;6(8):e23055. doi: 10.1371

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pmRNA-EGFP-A120

Plasmid

#225903

Purpose

In vitro transcription of cap-dependently translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pmRNA-mCherry-A120

Plasmid

#225904

Purpose

In vitro transcription of cap-dependently translated mRNA encoding mCherry.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
mCherry
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pmRNA-iSyn-EGFP

Plasmid

#225907

Purpose

In vitro transcription of cap-independently (synthetic IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

PABP motif
HRV-B3-derived synthetic IRES
EGFP
HBA1 UTR

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCK303.306

Plasmid

#242926

Purpose

Expression of an MS2 scRNA J306

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA MS2 J306

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCK677.102-110

Plasmid

#242928

Purpose

Expression of a boxB scRNA J102 and an MS2 scRNA J110

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA MS2 J110

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCK662.-1

Plasmid

#242922

Purpose

Reporter J1-pspAp-mRFP with 1bp deletion phase-shift

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

J1-pspAp.-1

Use

CRISPR and Synthetic Biology

Promoter

pspAp.-1

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pKO023

Plasmid

#242923

Purpose

Reporter J1-J3(122)-BBa_J23117 with Sth1 PAM

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

J1-J3(122)

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23117

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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