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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,785 results for: emb

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Showing: 5441 - 5460 of 11785 results

  1. TMED5-bio-His

    Plasmid
    #53338
    Purpose
    Expresses full-length Transmembrane emp24 domain-containing protein 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Publication
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    TMED5 (TMED5 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53338-plasmid-map-sequence-id-83068

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  5. pChlr-3SE

    Plasmid
    #52148
    Purpose
    Encodes module 3 with amino acids 12S-16E for recognition of nt 6G
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 97-132 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16E in Module 3
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. pChlr-5SR

    Plasmid
    #52162
    Purpose
    Encodes module 5 with amino acids 12S-16R for recognition of nt 4C
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 169-204 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12C to 12S and 16Q to 16R in Module 5
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pChlr-6SE

    Plasmid
    #52164
    Purpose
    Encodes module 6 with amino acids 12S-16E for recognition of nt 3G
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 205-240 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in Module 6
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. pChlr-8SE

    Plasmid
    #52172
    Purpose
    Encodes module 8 with amino acids 12S-16E for recognition of nt 1G
    Depositor
    Huimin Zhao
    Publication
    Abil et al J Biol Eng. 2014 Mar 1;8(1):7.
    Insert
    Pumilio homology domain amino acids 284-319 (PUM1 Human)
    Expression
    Bacterial
    Mutation
    Changed 12N to 12S and 16Q to 16E in module 8
    Available since
    April 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. PF3D7_0505700-COMP-blac-flag-his

    Plasmid
    #110988
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    conserved Plasmodium membrane protein (PF3D7_0505700 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    110988-plasmid-map-sequence-id-224478

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  15. PF3D7_1434400-COMP-blac-flag-his

    Plasmid
    #110981
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    conserved Plasmodium membrane protein (PF3D7_1434400 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110981-plasmid-map-sequence-id-224279

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  16. PF3D7_1333300-COMP-blac-flag-his

    Plasmid
    #110972
    Purpose
    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.
    Depositor
    Gavin Wright
    Publication
    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi
    Insert
    transmembrane protein Tmp21 homologue, putative (PF3D7_1333300 Synthetic)
    Tags
    Exogenous signal peptide of mouse origin and rat …
    Expression
    Mammalian
    Mutation
    All NXT/S sites mutated to NXA, where X is any am…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    110972-plasmid-map-sequence-id-224268

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  17. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid
    #228941
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228941-plasmid-map-sequence-id-469413

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  18. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid
    #228942
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Publication
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228942-plasmid-map-sequence-id-466826

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  19. pTol2-fli1-lsl-AmCyan-mScarlet; hsp70-zfCre-I-BFP (JDW 1234)

    Plasmid
    #229810
    Purpose
    A Tol2 based expression vector containing fli1 driven switch reporter (loxP flanked AmCyan followed by a mScarlet-I) and a hsp70-TagBFP-zCre in the opposite direction.
    Depositor
    Joshua Wythe
    Publication
    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043
    Insert
    loxp-AmCyan-stop-lox
    Use
    Tol2 based expression vector
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    229810-plasmid-map-sequence-id-461094

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Showing: 5441 - 5460 of 11785 results