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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,786 results for: sequence

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Showing: 5441 - 5460 of 13786 results

  1. pBJ-mPar3

    Plasmid
    #53230
    Purpose
    mammalian expression of mouse Par3
    Depositor
    Shaun Coughlin
    Insert
    Par3 (F2rl2 Mouse)
    Tags
    FLAG and bovine prolactin signal sequence (MDSKGS…
    Expression
    Mammalian
    Mutation
    Deletion of aa1-15 (native signal peptide)
    Available Since
    Aug. 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  2. pcDNA3.1-MS2-HA-HsRRP6_Z

    Plasmid
    #148131
    Purpose
    Mammalian Expression of HsRRP6
    Depositor
    Elisa Izaurralde
    Insert
    HsRRP6 (EXOSC10 Human)
    Expression
    Mammalian
    Mutation
    2 silent mutations compared to the sequence of is…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148131-plasmid-map-sequence-id-420961

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  3. AICSDP-77:TERF2-mEGFP

    Plasmid
    #168798
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human TERF2.
    Depositor
    Allen Institute for Cell Science
    Insert
    TERF2 (TERF2 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    April 14, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    168798-plasmid-map-sequence-id-330361

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  4. pGFP CrkII

    Plasmid
    #50730
    Purpose
    mammalian expression of GFP-CrkII. Note- This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.
    Depositor
    Kenneth Yamada
    Insert
    CrkII (CRK Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. G-SMARCA4-2

    Plasmid
    #166519
    Purpose
    scFv of a human scaffold targeting SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a4 isoform B. Antigen coverage aa 1451-1580 of 1647, Bromodomain.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166519-plasmid-map-sequence-id-329058

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  7. pAc5.1B-EGFP-DmAtx2_AE

    Plasmid
    #148548
    Purpose
    Insect Expression of DmAtx2
    Depositor
    Elisa Izaurralde
    Insert
    DmAtx2 (Atx2 Fly)
    Expression
    Insect
    Mutation
    Three silent mutations and two deletions compared…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148548-plasmid-map-sequence-id-474981

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  10. mCerulean-Golgi-7

    Plasmid
    #55371
    Purpose
    Localization: GalT Targeting Sequence, Excitation: 433, Emission: 475
    Depositor
    Michael Davidson
    Insert
    Golgi (B4GALT1 Human)
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    aa 1-82 of NM_001497.3
    Promoter
    CMV
    Available Since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid
    #174902
    Purpose
    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration
    Depositor
    Simon Elsaesser
    Insert
    Mma PylRS (pylS Methanosarcina mazei)
    Tags
    FLAG and NES
    Expression
    Mammalian
    Mutation
    Y306A, Y384F
    Promoter
    EF1
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174902-plasmid-map-sequence-id-344602

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  12. pET-24a-CXCR4

    Plasmid
    #133431
    Purpose
    Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.
    Depositor
    Gerhard Wagner
    Insert
    CXCR4 (CXCR4 Human)
    Expression
    Bacterial
    Available Since
    Jan. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    133431-plasmid-map-sequence-id-267724

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  14. KG#865

    Plasmid
    #110912
    Purpose
    Coding sequence for attaching the Auxin Inducible Degron to the C-terminus of a protein followed by a 5X Myc tag with spacers
    Depositor
    Kenneth Miller
    Insert
    Auxin Inducible Degron-5X Myc-Spacers for C-terminal attachment
    Expression
    Bacterial
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110912-plasmid-map-sequence-id-212549

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  15. KG#883

    Plasmid
    #110913
    Purpose
    Coding sequence for attaching the a 5X Myc tag with spacers followed by the Auxin Inducible Degron to the N-terminus of a protein
    Depositor
    Kenneth Miller
    Insert
    5X Myc-Spacers-Auxin Inducible Degron for N-terminal attachment
    Expression
    Bacterial
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110913-plasmid-map-sequence-id-212540

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  16. pEGB3_alpha2 35:PhyB:VP16 (GB2745)

    Plasmid
    #160630
    Purpose
    TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch
    Depositor
    Diego Orzaez
    Insert
    PhyBVP16
    Use
    Synthetic Biology
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    35S
    Available Since
    Jan. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    160630-plasmid-map-sequence-id-321969

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  17. pKG194

    Plasmid
    #69757
    Purpose
    Mammalian Expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and eGFP
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Available Since
    Nov. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pAc5.1B-DmPABPC1-V5His6_D

    Plasmid
    #146093
    Purpose
    Insect Expression of DmPABPC1
    Depositor
    Elisa Izaurralde
    Insert
    DmPABPC1 (pAbp Fly)
    Expression
    Insect
    Mutation
    one non-silent mutation G26S compared to the sequ…
    Available Since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    146093-plasmid-map-sequence-id-362948

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Showing: 5441 - 5460 of 13786 results