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pMSSM16: pCMV-HA-NLS-SpuFz1

Plasmid

#205271

Purpose

Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

HA, NLS(Makkerh)

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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LeGO-iG2-wPRE-pA

Plasmid

#60489

Purpose

eGFP-expressing lentiviral vector with IRES and pA-signal; suitable for use as NRTLV (non-reverse transcribable lentiviral vector)

Depositor

Boris Fehse

Article

Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

Insert

IRES-eGFP; wPRE; BGH-pA

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP670-2-T2A-HO1

Plasmid

#197250

Purpose

Expresses the protein of wmiRFP670-2-T2A-HO1 in neurons of C. elegans

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

wmiRFP670-2-T2A-HO1

Expression

Worm

Available Since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-BDFP1.6

Plasmid

#197199

Purpose

Expresses the protein of BDFP1.6 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

BDFP1.6

Use

AAV

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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Scarlet I-FRET 10-Venus

Plasmid

#139366

Purpose

FRET Standard

Depositor

Richard Day

Article

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Insert

Scarlet 10AA Venus

Expression

Mammalian

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNT3/Cre

Plasmid

#89654

Purpose

Expresses a non-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

Insert

non-targeting gRNA

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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CFP-FKBP

Plasmid

#162433

Purpose

for use in CID (chemically induced dimerization) or CIT (chemically induced trimerization) system

Depositor

Takanari Inoue

Article

Wu et al Nat Methods. 2020 Sep;17(9):928-936. doi

Insert

CFP-FKBP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

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UPmCh

Plasmid

#161004

Purpose

ubiquitous expression of PCP-mCherry

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PCP-mCherry

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pActinin-LSSmOrange

Plasmid

#37139

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange

Tags

human alpha-actinin

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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PNMT

Plasmid

#64078

Purpose

Expression of human Phenylethanolamine N-methyltransferase

Depositor

Vern Schramm

Article

Stratton et al ACS Chem Biol. 2017 Feb 17;12(2):342-346

Insert

Phenylethanolamine N-methyltransferase (PNMT Human, Synthetic)

Tags

Histidine tag

Expression

Bacterial

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only
pN1-QUEEN7mu

Plasmid

#129307

Purpose

Positive control for ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-7mu

Expression

Mammalian

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) CircRNA Mini Vector-ZKSCAN1 nt 548-1047

Plasmid

#60649

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pNR64

Plasmid

#79540

Purpose

Input plasmid: expresses two recombinases downstream of two inducible promoters. Same as Dual-Recombinase-Controller (plasmid #44456) from Endy Lab except with KanR instead of CamR on the backbone.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

TP901
BxbI

Tags

His tag and LAA degradation tag

Expression

Bacterial

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pJH726_pJH_LldR_PlldR-sfGFP.15TAG

Plasmid

#163730

Purpose

Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.15TAG

Expression

Bacterial

Mutation

gfp with first 15 leucine codons mutated to amber

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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UMsfG

Plasmid

#161005

Purpose

ubiquitous expression of MCP-sfGFP

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

MCP-sfGFP

Expression

Plant

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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ATG9A sgRNA

Plasmid

#207557

Purpose

pX330 expressing Cas9 and a sgRNA targeting the ATG9A locus

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

CACTGAATACCAGCGCCTAG

Expression

Mammalian

Promoter

CMV and U6

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Tags

mGFP

Expression

Mammalian

Promoter

cmv

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pMJ45: pRS426-His-MBP-SpuFz1_Native

Plasmid

#205252

Purpose

S. cerevisiae expression vector for SpuFz1 native guide

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Tags

10xHis, MBP

Expression

Yeast

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Golgi-Ras-LOCKR-S

Plasmid

#216499

Purpose

FRET-based reporter for Ras activity, Golgi localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

Golgi-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3 ER-Ras-LOCKR-S

Plasmid

#216500

Purpose

FRET-based reporter for Ras activity, ER localized

Depositor

David Baker

Article

Zhang et al Nat Biotechnol. 2024 Jan 25. doi: 10.103

Insert

ER-Ras-LOCKR-S

Expression

Mammalian

Available Since

April 3, 2024

Availability

Academic Institutions and Nonprofits only

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