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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,044 results for: cloning

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Showing: 5461 - 5480 of 63044 results

  3. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

    Plasmid
    #170209
    Purpose
    Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus
    Depositor
    Kelly Monk
    Publication
    Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.
    Insert
    slc1a3b (slc1a3b Zebrafish)
    Use
    Zebrafish
    Tags
    myrGCaMP6s-P2A-H2AmCherry
    Promoter
    slc1a3b
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    170209-plasmid-map-sequence-id-333268

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  6. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    112568-plasmid-map-sequence-id-217224

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  7. pJW2171

    Plasmid
    #163095
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Publication
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    163095-plasmid-map-sequence-id-320590

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  12. AAV-hACE2-cMYC-Flag

    Plasmid
    #158957
    Purpose
    Used for expression of human ACE2 with AAV packaging
    Depositor
    Akiko Iwasaki
    Insert
    Human ACE2 (ACE2 Human)
    Use
    AAV
    Tags
    Flag and MYC
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    158957-plasmid-map-sequence-id-310391

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  13. LentiGuide-Puro-P2A-EGFP_mRFPstuf

    Plasmid
    #137730
    Purpose
    Expresses customizable S. Pyogenes sgRNA from U6 promoter and PuroR-P2A-EGFP from EF-1a promoter. Stuffer contains mRFP from a bacterial promoter, enabling simple visual quality control step of sgRNA
    Depositor
    Fredrik Wermeling
    Publication
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, Mouse Targeting, and Syntheti…
    Expression
    Bacterial and Mammalian
    Available since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    137730-plasmid-map-sequence-id-310820

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  16. LiOn-CMV∞Cre

    Plasmid
    #154018
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing a functional Cre recombinase from a CMV promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Publication
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    Cre-FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154018-plasmid-map-sequence-id-308116

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  20. pcDNA5/FRT/TO-GAlphaoA-RLuc8

    Plasmid
    #140976
    Purpose
    Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlphaoA-RLuc8 (GNAO1 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140976-plasmid-map-sequence-id-300659

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Showing: 5461 - 5480 of 63044 results