We narrowed to 59,537 results for: cloning

1. MXS_PGK::tTA2-bGHpA

   Plasmid

   #62448

   Purpose

   MXS_chaining vector with PGK::tTA2-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the tetracycline transactivator with PGK promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pECNUS_MP01

   Plasmid

   #194350

   Purpose

   CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

   Depositor

   Eunyoung Chae

   Article

   Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

   Insert

   pcoCas9, FAST-Red
   

   Expression

   Plant

   Promoter

   RPS5A

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. MXS_PGK::rtTA3-bGHpA

   Plasmid

   #62446

   Purpose

   MXS_chaining vector with PGK::rtTA3-bGHpA

   Depositor

   Pierre Neveu

   Article

   Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

   Insert

   cassette containing the reverse tetracycline transactivator with PGK promoter
   

   Use

   Synthetic Biology

   Available Since

   May 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAMP-CY1_NF1 T2 (+minintr, KDR)

   Plasmid

   #119201

   Purpose

   transfer vector

   Depositor

   Helen Morrison

   Article

   Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

   Insert

   NF1 type 2 mini-gene (NF1 Human)
   

   Use

   Transfer vector

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-miniTurbo-V5-DYRK1B_IDG-K

   Plasmid

   #158183

   Purpose

   Gateway destination clone of DYRK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-DYRK1B (DYRK1B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICH41432

   Plasmid

   #50343

   Depositor

   Sylvestre Marillonnet, Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   3'UTR, polyadenylation signal/terminator, ocs (A. tumefaciens)
   

   Use

   Plant expression

   Mutation

   BsaI/BbsI sites removed by point-mutation

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCOND (Apr)

   Plasmid

   #110094

   Purpose

   Suicide vector based on the TetR/Pip OFF system.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Bacterial knockout generation

   Available Since

   May 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. p663-UBC-miniTurbo-V5-CDK18_IDG-K

   Plasmid

   #158180

   Purpose

   Gateway destination clone of CDK18 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-CDK18 (CDK18 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-D-TOPO-kcnn2

   Plasmid

   #164958

   Purpose

   Zebrafish kcnn2 gene CDS

   Depositor

   GuangJun Zhang

   Article

   Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

   Insert

   kcnn2 (kcnn2 Zebrafish)
   

   Use

   Pcr cloning vector

   Available Since

   March 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCS2+/N-SNAPf

    Plasmid

    #184415

    Purpose

    vector backbone to clone insert downstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS2+/C-SNAPf

    Plasmid

    #184416

    Purpose

    vector backbone to clone insert upstream of SNAPf tag (SNAPf tag codon optimized for Xenopus), for mRNA synthesis

    Depositor

    Ann Miller

    Article

    Dudley et al STAR Protoc. 2022 Aug 18;3(3):101622. do

    Type

    Empty backbone

    Use

    For mrna synthesis

    Tags

    SNAPf-tag

    Mutation

    Codon optimized for expression in Xenopus laevis …

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCZGY2838

    Plasmid

    #87759

    Purpose

    Pmyo-3-PH::miniSOG, expression of membrane targeted PH-miniSOG in body wall muscles.

    Depositor

    Andrew Chisholm

    Article

    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

    Insert

    Pmyo-3-PH-miniSOG
    

    Tags

    PH

    Expression

    Worm

    Promoter

    Pmyo-3

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5/FRT/TO HIS DNAJB6b

    Plasmid

    #19555

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6b
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. p221z-AtMIR390a

    Plasmid

    #118388

    Purpose

    Entry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.

    Depositor

    Ari Pekka Mähönen

    Article

    Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

    Insert

    AtMIR390a backbone
    

    Use

    RNAi

    Available Since

    Sept. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pICH44022

    Plasmid

    #50330

    Depositor

    Sylvestre Marillonnet

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, P19 suppressor of gene silencing (Tomato Bushy Stunt Virus)
    

    Use

    Plant expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. MXS_PGK::CreERT2-bGHpA

    Plasmid

    #62444

    Purpose

    MXS_chaining vector with PGK::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with PGK Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMBD-Gate2

    Plasmid

    #25947

    Depositor

    Kamil Onder

    Article

    Maier et al Assay Drug Dev Technol. 2010 Oct;8(5):62

    Insert

    
    

    Tags

    GAL4 Fusion Protein

    Expression

    Mammalian

    Available Since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. psgRNA-ccdB

    Plasmid

    #99915

    Purpose

    Basic cloning vector for the expression of imaging-based optimization sgRNA

    Depositor

    Yujie Sun

    Article

    Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. MXS_CMV::CreERT2-bGHpA

    Plasmid

    #62443

    Purpose

    MXS_chaining vector with CMV::CreERT2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tamoxifen inducible Cre recombinase with CMV Promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. GGE152

    Plasmid

    #120752

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_8UASxpr-TEF)

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    P2_8UASxpr-TEF
    

    Use

    Cloning/assembly plasmid

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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