We narrowed to 6,252 results for: nls

1. Recruited polymerase expression - pCMV-T7-Phi29-CC(N5) (LM2726)

   Plasmid

   #208971

   Purpose

   Recruited Phi29 DNA polymerase (-exo) with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(-exo;D169A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

   Plasmid

   #208970

   Purpose

   Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Phi29(+exo)-BPNLS-CC(N5)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. CRISPRoff-v2.1

   Plasmid

   #167981

   Purpose

   Expresses CRISPRoff-v2.1 (DNMT3A-DNMT3L-XTEN80-dCas9-HA-2xNLS-BFP-KRAB) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   CRISPRoff-v2.1 (DNMT3A-DNMT3L-dCas9-BFP-KRAB)
   

   Tags

   2xNLS, DNMT3A-DNMT3L, HA, KRAB, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TETv4

   Plasmid

   #167983

   Purpose

   Expresses TETv4 (TET1CD-XTEN80-dCas9-3xNLS-P2A-BFP) downstream of the CAG promoter

   Depositor

   Luke Gilbert

   Article

   Nuñez et al Cell. 2021 Apr 7. pii: S0092-8674(21)003

   Insert

   TETv4 (TET1CD-XTEN80-dCas9)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   3xNLS, TET1 catalytic domain, and tagBFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   May 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

   Plasmid

   #169912

   Purpose

   Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   dCjCas9
   

   Use

   CRISPR

   Tags

   10XGCN4, 2xSV40NLS, and SV40NLS-HA

   Expression

   Mammalian

   Mutation

   D8A, H559A in cjCas9

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-UCOE-SFFV-dCas9-P2A-EGFP

   Plasmid

   #188898

   Purpose

   Human lentiviral vector for expression of dCas9 with a C-terminal HA-2xNLS and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   HA-2xNLS and P2A-GFP

   Promoter

   SFFV

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SPACE (pRZ1813)

   Plasmid

   #140242

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPACE-NG (pRZ5150)

   Plasmid

   #140244

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9_NG-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE_NG
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, NG mutations in SpCas9, D10A in Sp…

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Polymerase-unfused click editor - pCMV-T7-PCV2-nCas9 (LM2702)

   Plasmid

   #208961

   Purpose

   Polymerase-unfused click editor comprised of PCV2 fused to nCas9, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available Since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nickase SpCas9 - pCMV-T7-nSpCas9(H840A)-P2A-GFP (HES140)

    Plasmid

    #208976

    Purpose

    Nickase SpCas9 (H840A) with a P2A GFP and 3xFLAG, expressed from a CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    nSpCas9(H840)-BPNLS-P2A-GFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188765

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    SFFV

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PE2-p53DD

    Plasmid

    #196582

    Purpose

    Mammalian expression of SpCas9 prime editor 2 with P2A-p53DD

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    PE2-P2A-mp53DD
    

    Expression

    Mammalian

    Mutation

    Δ40-903

    Promoter

    CMV

    Available Since

    March 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR_UCOE-EF1a-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

    Plasmid

    #188769

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    EF1a

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188772

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    CMV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPACE-VRQR (pRZ5137)

    Plasmid

    #140245

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE_VRQR
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, VRQR mutations in SpCas9, D10A in …

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188771

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Promoter

    EFS

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. SPACE-ΔUGI (pRZ1836)

    Plasmid

    #140243

    Purpose

    CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    SPACE-ΔUGI
    

    Expression

    Mammalian

    Mutation

    V82G in TadA*, D10A in Cas9, R187W in pmCDA1

    Promoter

    CMV

    Available Since

    June 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. CGBE1-VRQR (pBM1192)

    Plasmid

    #140254

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_VRQR-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid

    #208962

    Purpose

    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRGEN-CMV-VPR-WT CjCas9 V-WT

    Plasmid

    #169914

    Purpose

    Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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