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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,277 results for: loxP

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Showing: 5501 - 5520 of 11277 results

  2. Halo-53BP1 HRD

    Plasmid
    #207087
    Purpose
    Homologous recombination donor for insertion of a HaloTag at the N-terminus of the endogenous 53BP1 locus.
    Depositor
    Jens Schmidt
    Publication
    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HaloTag with internal PuroR cassette flanked by human 53BP1 locus sequences
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    Endogenous
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207087-plasmid-map-sequence-id-431238

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  4. pCRISPRai

    Plasmid
    #189938
    Purpose
    Expresses CRISPRai cassette under EF1a promoter
    Depositor
    Yu-Chen Hu
    Publication
    Truong et al Nucleic Acids Res. 2019 Jul 26;47(13):e7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CRISPRai cassette
    Expression
    Mammalian
    Promoter
    CMV enhancer-rat EF1 alpha
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    189938-plasmid-map-sequence-id-373416

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  8. ROSA26(MultiFPsΔPuro)

    Plasmid
    #140759
    Purpose
    Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).
    Depositor
    Hideyuki Okano
    Publication
    Serizawa et al Development. 2019 Nov 4;146(21). pii: de
    Inserts
    puromycin-N-acetyltransferase
    Venus
    mCherry
    mCerulean
    Use
    Mouse Targeting
    Promoter
    CAGGS
    Available since
    June 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140759-plasmid-map-sequence-id-311014

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  10. pGS-CD64-2

    Plasmid
    #109191
    Purpose
    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system
    Depositor
    Monika Golas
    Publication
    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do
    Insert
    Full-length engineered CD64, derived from human CD64
    Tags
    Twin-Strep
    Expression
    Insect
    Mutation
    engineered for efficient expression, see publicat…
    Promoter
    Polyhedrin
    Available since
    April 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    109191-plasmid-map-sequence-id-233794

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  11. pL452-Sf3b1-K700E

    Plasmid
    #90425
    Purpose
    selectable HDR vector to introduce K700E mutation within mus Sf3b1 gene (for use with sgSf3b1(T1) and pL452(hygro)-Sf3b1-K700K plasmids enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Publication
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90425-plasmid-map-sequence-id-182684

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  14. pWPT-/CGA-mEGFP-IRES-mCherry

    Plasmid
    #49233
    Purpose
    Lentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.
    Depositor
    Clifford Wang
    Publication
    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110
    Inserts
    mEGFP
    mCherry
    Use
    Lentiviral and Synthetic Biology
    Tags
    SfuI site to create C-terminal fusions
    Expression
    Mammalian
    Promoter
    EF1-short
    Available since
    Dec. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    49233-plasmid-map-sequence-id-73655

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  18. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid
    #234448
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Publication
    Aggarwal et al Nat Methods. 2026 Feb;23(2):417-425. doi
    Insert
    iGluSnFR4f-PDGFR
    Use
    AAV and Cre/Lox
    Tags
    IgK chain and Myc epi tag-PDGFR TM Domain
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234448-plasmid-map-sequence-id-465743

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  19. pAAV-hSyn-DIO-FLAG-KORD-P2A-3xHA-hM3D(Gq)

    Plasmid
    #220611
    Purpose
    Neuron-specific, Cre-dependent co-expression of FLAG-tagged inhibitory KORD DREADD receptor, and 3x HA-tagged excitatory hM3D(Gq) DREADD receptor.
    Depositor
    Sam Golden
    Publication
    Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121
    Insert
    FLAG-KORD-P2A-3x HA-hM3D(Gq)
    Use
    AAV and Cre/Lox
    Tags
    FLAG; ArgiNLS
    Expression
    Mammalian
    Promoter
    hSyn
    Available since
    Aug. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    220611-plasmid-map-sequence-id-441218

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  20. pAAV-hsyn-DIO-Rpl22l1-3Flag-2A-eGFP-WPRE

    Plasmid
    #214265
    Purpose
    Expression of a Flag-tagged Rpl22I1 protein in cells expressing Cre recombinase allowing for the harvesting of actively transcribed mRNAs in that cell type.
    Depositor
    D. James Surmeier
    Insert
    Flag-tagged Rpl22I1 protein expressing Cre Recombinase (Rpl22 Mouse)
    Use
    AAV
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    Synapsin
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    214265-plasmid-map-sequence-id-429396

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Showing: 5501 - 5520 of 11277 results