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plenti-UBC-gate-3xHA-pGK-PUR

Plasmid

#107393

Purpose

Lentiviral gateway destination vector

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral; Gateway destination

Tags

3xHA

Expression

Mammalian

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-Q23

Plasmid

#40261

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

EGFP

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pHM6-Q74

Plasmid

#40264

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q74 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pHM6-Q23

Plasmid

#40263

Depositor

David Rubinsztein

Article

Narain et al J Med Genet. 1999 Oct;36(10):739-46.

Insert

HTT partial exon 1 Q23 (HTT Human)

Tags

6x His and HA

Expression

Mammalian

Mutation

expanded poly-Q

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti_CMV: Flag-Akaluc_SV40:PuroR

Plasmid

#183048

Purpose

Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

Depositor

Antonio Amelio

Article

Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

Insert

AkaLuc

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pACS141

Plasmid

#204597

Purpose

Histone H3.3-V5

Depositor

Tom Misteli

Article

Schibler et al Elife. 2023 May 23;12:e80653. doi: 10.75

Insert

human histone H3.3 (H3-3A Human)

Tags

human histone H3.3 - V5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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wtTDP43tdTOMATOHA

Plasmid

#28205

Depositor

Zuoshang Xu

Article

Yang et al PLoS One. 2010 . 5(12):e15878.

Insert

Tar DNA binding protein (TARDBP Human)

Tags

HA tag and tdtomato

Expression

Mammalian

Available Since

June 1, 2011

Availability

Academic Institutions and Nonprofits only

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GFP-HP1alpha

Plasmid

#17652

Depositor

Tom Misteli

Article

Cheutin et al Science. 2003 Jan 31. 299(5607):721-5.

Insert

HP1 alpha (CBX5 Human)

Tags

GFP and His

Expression

Mammalian

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pTP264

Plasmid

#149334

Purpose

Bacterial expression of Biotin ligase BirA

Depositor

Dirk Görlich

Article

Pleiner et al Elife. 2015 Dec 3;4:e11349. doi: 10.7554

Insert

Biotin ligase BirA

Tags

His14 and bdNEDD8

Expression

Bacterial

Available Since

June 18, 2020

Availability

Academic Institutions and Nonprofits only

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PER2:Luc Plasmid

Plasmid

#206875

Purpose

Per2 promoter luciferase reporter

Depositor

Michelle Farkas

Article

Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

Insert

Period 2 (Per2 Mouse)

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-Tmprss6(Mask)

Plasmid

#18790

Depositor

Bruce Beutler

Article

Du et al Science. 2008 May 23. 320(5879):1088-92.

Insert

Type II transmembrane serine protease 6 (Tmprss6 Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 567-811 and replaced with 28 …

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
FL-fibulin-3-V5 pcDNA4

Plasmid

#29701

Depositor

Mariano Viapiano

Article

Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

Insert

EFEMP1 (EFEMP1 Human)

Tags

V5

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only
pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Article

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-LaG17-tTA synNotch (LA1)

Plasmid

#162230

Purpose

Lentiviral vector for the expression of GFP nanobody LaG17 tTA SynNotch: pHR_EF1a-->myc-LaG17-tTA synNotch (LA1) in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

LaG17 GFP nanobody SynNotch tTA

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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TagBFP2-CAAX

Plasmid

#116856

Purpose

plasma membrane marker

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

HRAS

Tags

TagBFP2

Expression

Mammalian

Mutation

Amino acids 172-189

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pDG02583

Plasmid

#104129

Purpose

Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

NEDD8-specific protease 1 (NEDP1)

Tags

14x Histidine tag, Maltose binding protein, and S…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pFW2100

Plasmid

#224212

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pMCSG53-DsbC

Plasmid

#186623

Purpose

E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

Depositor

Alexei Savchenko

Article

Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

Type

Empty backbone

Tags

DsbC-6His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-rabbit-Nb-Hia5

Plasmid

#239627

Purpose

Rhamnose inducible expression plasmid for anti-rabbit nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Sidhwani et al bioRxiv 2025.07.01.662447; doi: https://

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Rabbit Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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