We narrowed to 47,182 results for: uros

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pGL3-MTS-CYTB-W77-L15-G1333C-UGI-Puro

Plasmid

#198848

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(CYTB) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

CYTB-W77-L15 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-COX1-W25-L15-G1333C-UGI-Puro

Plasmid

#198842

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX1) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

COX1-W25-L15 TALEN

Expression

Mammalian

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-MTS-ND6-W82-L15-G1333N-UGI-Puro

Plasmid

#198860

Purpose

Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

Depositor

Bin Shen

Article

Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

Insert

ND6-W82-L15 TALEN

Expression

Mammalian

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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PL752-PTPRO-LA-p2a-tdTomato-hGHpA-PGK-puroR-PTPRO-RA

Plasmid

#191686

Purpose

For knocking p2a-tdTomato into PTPRO locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

PTPRO homology arms and p2a-tdTomato

Expression

Mammalian

Mutation

WT

Available Since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

Plasmid

#177145

Purpose

Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

Depositor

Robert Sobol

Article

Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

Insert

PolB (POLB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCT-CMV-copGFP-Polß(L301R/V303R/V306R)-puro

Plasmid

#128666

Purpose

mammalian expression of copGFP-Polß(L301R/V303R/V306R: TM) protein with puromcyin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

copGFP

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

May 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-Polß(L301R/V303R/V306R)-IRES-puro

Plasmid

#128657

Purpose

mammalian expression of Flag-Polß(L301R/V303R/V306R) protein with puromycin selection

Depositor

Robert Sobol

Article

Fang et al Nat Commun. 2014 Nov 26;5:5513. doi: 10.

Insert

PolB (POLB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

L301R/V303R/V306R

Promoter

CMV

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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NFATc4-CRISPR-gRNA#exon5-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124290

Purpose

sgRNA against huiman NFATc4

Depositor

Katalin Josvay, Csaba Vizler

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
NFATc4-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124289

Purpose

sgRNA against human NFATc4

Depositor

Katalin Josvay, Csaba Vizler

Insert

NFATc4 nuclear factor of activated T cells (NFATC4 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

Plasmid

#31482

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce Target) EF Puro

Use

Lentiviral

Mutation

mCherry has M9S and M16L mutations. This is what …

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

Plasmid

#86457

Purpose

Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

Depositor

Ralf Kuehn

Article

Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

BFP

Use

CRISPR

Expression

Mammalian

Promoter

Pgk

Available Since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ2815 pPB: CAG-Puro-WPRE PGK-KRAB-tagBFP-SpdCas9

Plasmid

#84245

Purpose

Expresses direct fusion KRAB-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

KRAB-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, KRAB, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

Plasmid

#42337

Purpose

Dual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Available Since

Feb. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Ani target) Ef1a Puro

Plasmid

#31480

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (ani target)

Use

Lentiviral

Mutation

mCherry has M9S, M16L to reduce background mCherr…

Available Since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a Puro

Plasmid

#31482

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce Target) EF Puro

Use

Lentiviral

Mutation

mCherry has M9S and M16L mutations. This is what …

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

Plasmid

#213026

Purpose

Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

Depositor

Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag

Tags

3xFlag (ORF2p)

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

Plasmid

#217306

Purpose

sgRNA mcherry + Puromycin

Depositor

Luke Gilbert

Insert

pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry

Use

CRISPR

Promoter

U6, Ef1alpha

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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