We narrowed to 19,653 results for: Pol
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Plasmid#108402PurposeExpress putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157DepositorAvailable SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only
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pSpdCas9-huTET1CD-T2A-GFP (PX458)
Plasmid#129025Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-GFP (PX458)
Plasmid#129025Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)
Plasmid#129027Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-huTET1CD-T2A-mCherry(PX458)
Plasmid#129027Purposetargeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNADepositorInsertdCas9-huTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A)PromoterCbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-WT
Plasmid#171377PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
TagsBAD tagExpressionYeastPromoterURA3Available SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYeDP60-hATP13A2-WT
Plasmid#171377PurposeA modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2, followed by a thrombin cleavage site and a C-terminal BAD tag. DOI: 10.21769/BioProtoc.3888DepositorInserthuman ATP13A2 variant 2 wild-type
TagsBAD tagExpressionYeastPromoterURA3Available SinceSept. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-GFP (PX458)
Plasmid#129026Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-GFP (PX458)
Plasmid#129026Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-EGFPExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)
Plasmid#169182PurposeBaculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cellsDepositorInsertnsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
TagsHis6-3xFlagExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)
Plasmid#169182PurposeBaculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cellsDepositorInsertnsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
TagsHis6-3xFlagExpressionInsectMutationCodon optimised for insect cells (Spodoptera frug…PromoterPolyhedrinAvailable SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.SUZ12
Plasmid#125172PurposeExpresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorAvailable SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.SUZ12
Plasmid#125172PurposeExpresses human SUZ12 in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorAvailable SinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
Tagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
cryS96
Plasmid#67519PurposeαB-crystallin minipeptide fused to ELP (S96).DepositorInsertmini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Synthetic, Human)
Tagselastin-like polypeptide (ELP) S96ExpressionBacterialMutationmini-αB-crystallin contains residues 73–92 of aB-…PromoterT7Available SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)
Plasmid#129028Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpdCas9-hudTET1CD-T2A-mCherry(PX458)
Plasmid#129028Purposeinactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNADepositorInsertdCas9-hudTET1CD, SgRNA cloning site
TagsHA-Tag, NLS and T2A-mCherryExpressionMammalianMutationdCas9 (D10A;H840A), catalytic domain huTET1 inact…PromoterCbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)Available SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_4106-eGFP-3HA
Plasmid#108399PurposeExpress cytoplasmic tail human PC1 from amino acid 4106 to 4302DepositorAvailable SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-hPC1_4106-eGFP-3HA
Plasmid#108399PurposeExpress cytoplasmic tail human PC1 from amino acid 4106 to 4302DepositorAvailable SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CD16.7-hPC1-eGFP-3HA
Plasmid#108401PurposeExpress cytoplasmic tail human PC1 from amino acid 4077 to 4302 with a membrane anchorDepositorAvailable SinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only