We narrowed to 6,140 results for: arl

1. pGL3-NurRE3

   Plasmid

   #208253

   Purpose

   pGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cells

   Depositor

   Douglas Kojetin

   Article

   Munoz-Tello et al J Med Chem. 2020 Dec 24;63(24):15639-156

   Insert

   3X Nur response element LUC
   

   Tags

   No

   Expression

   Mammalian

   Promoter

   SV40 early promoter

   Available Since

   Feb. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1

   Plasmid

   #162164

   Purpose

   Selectable homology donor template for Aag2 cell AGO1

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   RFP
   3xFLAG
   

   Use

   CRISPR and Cre/Lox; Homology donor template

   Tags

   AGO1 homology and loxP

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and endogenous AGO1

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV hU6-gRNA hUbC-VP64-dSaCas9-VP64-T2A-Thy1.1

   Plasmid

   #194279

   Purpose

   Expresses gRNA and VP64-dSaCas9-VP64 from lentiviral vector

   Depositor

   Charles Gersbach

   Article

   McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

   Inserts

   humanized VP64 dSaCas9 VP64 T2A Thy1.1
   gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   hU6 and hUbC

   Available Since

   Feb. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKRG3-mU6-PUb-3xFLAG-hSpCas9

   Plasmid

   #162161

   Purpose

   Expresses 3xFLAG tagged hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

   Depositor

   Charles Rice

   Article

   Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

   Inserts

   hSpCas9
   sgRNA
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Insect

   Promoter

   Ae. aegypti PUb and Ae. aegypti U6

   Available Since

   Jan. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRVdGL-4Flpo

   Plasmid

   #98040

   Purpose

   Second-generation rabies viral vector genome

   Depositor

   Ian Wickersham

   Article

   Chatterjee et al Nat Neurosci. 2018 Apr;21(4):638-646. do

   Insert

   Flpo
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-MDFI

   Plasmid

   #207836

   Purpose

   Tagged MDFI for western blotting and imaging

   Depositor

   Charles Cox

   Article

   Zhou et al Science. 2023 Aug 18;381(6659):799-804.

   Insert

   MDFI (MDFI Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. cyt-GafD-mTurboID-V5

   Plasmid

   #184640

   Purpose

   Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   cyt-GlycoID-V5
   

   Tags

   Nuclear export sequence and V5 tag

   Expression

   Mammalian

   Mutation

   GafD -> expressed aa22-178 ; BirA* -> expre…

   Promoter

   CMV

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-tetO-lox-hKLF4

   Plasmid

   #20727

   Depositor

   Rudolf Jaenisch

   Article

   Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CaMKIIa-ChRmine-eYFP-WPRE

   Plasmid

   #130988

   Purpose

   AAV vector to drive the expression of ChRmine-eYFP under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Insert

   ChRmine
   

   Use

   AAV

   Tags

   eYFP

   Promoter

   CaMKIIa

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry

    Plasmid

    #35512

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123T and T159C

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CaMKIIa-C1V1 (t/t)-TS-mCherry

    Plasmid

    #35500

    Purpose

    AAV expression of CaMKII-driven chimeric opsin variant C1V1 (E122T/E162T) for fast and potent optical excitation at red-shifted wavelengths.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    ChR1-VChR1 Chimera
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E122T and E162T

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pECE M2-SH3PXD2A WT

    Plasmid

    #69813

    Purpose

    Expresses M2-SH3PXD2A in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    SH3 and PX domain-containing protein 2A (SH3PXD2A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SV40 early promoter

    Available Since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Ef1a-sCreDIO hChR2(H134R)-EYFP

    Plasmid

    #55642

    Purpose

    sCre-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV; Scre-dependent

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. nuc-GafD-mTurboID-HA

    Plasmid

    #184641

    Purpose

    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

    Depositor

    Charlie Fehl

    Article

    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

    Insert

    nuc-GlycoID-HA
    

    Tags

    3xHA tag and Nuclear localization sequence

    Expression

    Mammalian

    Mutation

    GafD -> expressed 22-178 ; BirA* -> express…

    Promoter

    CMV

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CaMKIIa-hChR2(C128S/D156A)-EYFP

    Plasmid

    #35501

    Purpose

    AAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.

    Depositor

    Karl Deisseroth

    Article

    Yizhar et al Nature. 2011 Jul 27;477(7363):171-8. doi

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128S and D156A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-memRFP-3xPax7gRNA

    Plasmid

    #224569

    Purpose

    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.

    Depositor

    Erica Hutchins

    Article

    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

    Insert

    mRFP1
    

    Use

    CRISPR

    Tags

    Membrane localization signal

    Mutation

    Codon optimized

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Orai1-jGCaMP8f V203Y

    Plasmid

    #231631

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

    Insert

    Orai1-jGCaMP8f V203Y (ORAI1 Synthetic, Human)
    

    Tags

    jGCaMP8f V203Y

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUW-tetO-lox-hOCT4

    Plasmid

    #20728

    Depositor

    Rudolf Jaenisch

    Article

    Soldner et al Cell. 2009 Mar 6. 136(5):964-77.

    Insert

    OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CaMKIIa-eArchT 3.0-EYFP

    Plasmid

    #35513

    Purpose

    Lentiviral expression of CaMKIIa-driven ArchT 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArchT 3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-ChRmine-eYFP-WPRE

    Plasmid

    #130992

    Purpose

    AAV vector to drive the expression of ChRmine-eYFP under the control of human synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

    Insert

    ChRmine
    

    Use

    AAV

    Tags

    eYFP

    Promoter

    hSyn

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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