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pEntr-Nkx2-5flbio

Plasmid

#32969

Depositor

William Pu

Article

He et al Proc Natl Acad Sci U S A. 2011 Apr 5. 10

Insert

Nkx2-5 (Nkx2-5 Mouse)

Use

Gateway

Tags

flag-bio

Available Since

Jan. 9, 2012

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(WT)

Plasmid

#111323

Purpose

for T7 transcription of wt actin pre-mRNA

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Expression

Bacterial

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pMB60

Plasmid

#47941

Purpose

Can be used to generate synthetic guide in vitro from T7 promotor

Depositor

Mike Boxem

Article

Waaijers et al Genetics. 2013 Aug 26.

Insert

synthetic guide RNA

Use

CRISPR

Expression

Worm

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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U6-TM2Emp-6T_iBlue

Plasmid

#200235

Purpose

Backbone for cloning short RNA triggers that activate iSBH-sgRNAs

Depositor

Tatjana Sauka-Spengler

Article

Pelea et al bioRxiv 2023.05.08.539738

Type

Empty backbone

Expression

Mammalian

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBLO5.01

Plasmid

#121418

Purpose

pUC19_BsaI-dCas9-BsaI

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

dcas9

Expression

Bacterial

Promoter

lac

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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RCAS(A) mTcf4B (CT#1126)

Plasmid

#14019

Depositor

Cliff Tabin

Article

Kardon et al Dev Cell. 2003 Dec . 5(6):937-44.

Insert

Tcf4B (Tcf7l2 Mouse)

Use

Retroviral; Avian expression

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pET28CLY6

Plasmid

#21765

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 6 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pLew100_myc_BirA*

Plasmid

#41716

Depositor

Graham Warren

Article

Morriswood et al Eukaryot Cell. 2012 Dec 21.

Type

Empty backbone

Use

T. brucei expression

Promoter

PARP

Available Since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pFLIPmal-40uDelta1YFP

Plasmid

#18853

Depositor

Wolf Frommer

Article

Kaper et al Biotechnol Biofuels. 2008 Jun 3. 1(1):11

Insert

optimized maltose FRET sensor

Tags

CFP and YFP

Expression

Bacterial

Mutation

N227A;

Available Since

Oct. 23, 2008

Availability

Academic Institutions and Nonprofits only
pET28CLY7

Plasmid

#21766

Depositor

Maarten Merkx

Article

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 7 SGGSGG repeats

Tags

His

Expression

Bacterial

Available Since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
p316CUP1-3SGFP.SG

Plasmid

#1207

Depositor

Susan Lindquist

Article

Liu et al Nature 1999 Aug 5;400(6744):573-6.

Insert

sup35 NM domain (SUP35 Budding Yeast)

Tags

GFP

Expression

Yeast

Available Since

May 25, 2005

Availability

Academic Institutions and Nonprofits only

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P1

Plasmid

#87089

Purpose

This is the P1 part of the Bcl-2 promoter with a luciferase reporter in pGL3Basic plasmid

Depositor

Yohannes Tesfaigzi

Article

Bredow et al Gene. 2007 Dec 1;404(1-2):110-6. Epub 20

Type

Empty backbone

Expression

Mammalian

Promoter

Bcl-2 Promoter

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only
pWT055g

Plasmid

#96864

Purpose

sgRNA-HEK3

Depositor

David Liu

Article

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Insert

sgRNA-HEK3

Expression

Mammalian

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pClodANL-Cherry

Plasmid

#107870

Purpose

Bacterial expression of cherry-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

Cherry (red fluorescent protein)

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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U6>XX

Plasmid

#87542

Purpose

Component 1 of 2 for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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XX>sgRNA F+E

Plasmid

#87543

Purpose

Template 2 (of 2) for U6>sgRNA cassette OSO-PCR

Depositor

Lionel Christiaen

Article

Gandhi et al Dev Biol. 2017 May 1;425(1):8-20. doi: 1

Type

Empty backbone

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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AaHsp70Aa-1373-FFluc

Plasmid

#52911

Purpose

Ae aegypti hsp70Aa promoter driving firefly luciferase

Depositor

Zach Adelman

Article

Gross et al J Med Entomol. 2009 May;46(3):496-504.

Insert

Aa Hsp70Aa-1373

Use

Luciferase

Promoter

Aa Hsp70Aa-1373

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pBS cTbx3 (CT#23)

Plasmid

#13920

Depositor

Cliff Tabin

Article

Logan et al Development. 1998 Aug . 125(15):2825-35.

Insert

Tbx3 (TBX3 Chicken)

Expression

Bacterial

Available Since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pSEYC68-HA-TOR2 (pAS21)

Plasmid

#39207

Depositor

Michael Hall

Article

Kunz et al J Biol Chem. 2000 Nov 24;275(47):37011-2

Insert

TOR2 (TOR2 Budding Yeast)

Tags

HA

Expression

Yeast

Promoter

GAL

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only
pBLO 9.2_bCas9-CP199

Plasmid

#121496

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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