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pYPQAT52

Plasmid

#223424

Purpose

T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT53

Plasmid

#223425

Purpose

T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; Hygromycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYPQAT54

Plasmid

#223426

Purpose

T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

2x35s-ecTadA8e-dLbCas12a-D156R-2x35s-crRNA scaffold

Use

CRISPR

Tags

NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-S7-mRuby2

Plasmid

#233236

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S7-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-S4-mRuby2

Plasmid

#233235

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S4-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vinculin-Clover-CC-S2-mRuby2

Plasmid

#233234

Purpose

Vinculin FRET tension sensor with coiled-coil S2 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S2-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S2-mRuby2-Vt

Plasmid

#233227

Purpose

Vinculin FRET tension sensor with coiled-coil S2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S2-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-M2-mRuby2-Vt

Plasmid

#233231

Purpose

Vinculin FRET tension sensor with coiled-coil M2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-M2-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vinculin-Clover-CC-Mad-mRuby2

Plasmid

#233239

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-Mad-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S1-mRuby2-Vt

Plasmid

#233230

Purpose

Vinculin FRET tension sensor with coiled-coil S1 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S1-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S0-mRuby2-Vt

Plasmid

#233226

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S0-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S4-mRuby2-Vt

Plasmid

#233228

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S4-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S7-mRuby2-Vt

Plasmid

#233229

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S7-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-Mad-mRuby2-Vt

Plasmid

#233232

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-Mad-mRuby2-Vt

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vinculin-Clover-CC-S0-mRuby2

Plasmid

#233233

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S0-mRuby2

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGM65

Plasmid

#220170

Purpose

CRISRPi guide RNA against NACA

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

NACA (sgRNA) (NACA Human)

Use

Lentiviral

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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MZB215

Plasmid

#221767

Purpose

Bacterial expression of human Arf6 (Q67L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf6 (ARF6 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q67L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB218

Plasmid

#221769

Purpose

Bacterial expression of human Arf4 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf4 (ARF4 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB219

Plasmid

#221770

Purpose

Bacterial expression of human Arf5 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arf5 (ARF5 Human)

Tags

CPD-10xHis

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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MZB220

Plasmid

#221771

Purpose

Bacterial expression of human Arl1 (Q71L) with C-terminal 'self-cleaving' CPD-10xHis tag

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

Arl1 (ARL1 Human)

Tags

CPD

Expression

Bacterial

Mutation

Q71L

Promoter

T7 Promoter

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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