We narrowed to 3,027 results for: BFP

1. pPB_mU6_enh-gRNA_Puro-T2A-BFP

   Plasmid

   #235571

   Purpose

   Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   sgRNA backbone
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIX403-EBFP2-ccdB-G418

   Plasmid

   #158556

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4TO-24xGCN4_v4-BFP-24xPP7

   Plasmid

   #74929

   Purpose

   Translation reporter

   Depositor

   Marvin Tanenbaum

   Article

   Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

   Insert

   24xGCN4_v4-BFP-24xPP7
   

   Expression

   Mammalian

   Available Since

   May 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC13N-dCas9-BFP-KRAB

   Plasmid

   #127968

   Purpose

   constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

   Depositor

   Martin Kampmann

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   CLYBL-CAG-dCas9-NLS-BFP-KRAB
   

   Use

   TALEN

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pME-dCas9-BFP-KRAB

   Plasmid

   #221502

   Purpose

   CRISPRi machinery fish codon optimized

   Depositor

   Alessandro Ori

   Insert

   dCas9-tagBFP-KRAB
   

   Use

   Fish expression

   Mutation

   D10A and H839A point mutations

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE3G-PE2-P2A-BFP

   Plasmid

   #231580

   Purpose

   Expresses the PE2 prime-editing machinery fused to BFP (PE2-P2A-BFP), under the control of a TRE3G promoter. This promoter is responsive to doxycycline bound to the rtTA protein

   Depositor

   Jesse Engreitz

   Article

   Martyn et al bioRxiv [Preprint]. 2023 Dec 21:2023.12.

   Insert

   PE2-P2A-BFP
   

   Use

   Lentiviral

   Tags

   P2A-BFP

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

   Plasmid

   #67980

   Purpose

   Cas9 activity reporter with BFP and GFP.

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKBFP2AGFP cassette, WPRE
   

   Use

   Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKLV2-U6gRNA5(SMARCA2(46))-PGKpuro2ABFP-W

   Plasmid

   #200465

   Purpose

   Lentiviral vector expressing gRNA targeting human SMARCA2

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   SMARCA2(46) (SMARCA2 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIP-SFFV-mtagBFP-2A RIGI WT

   Plasmid

   #167289

   Purpose

   Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid

    #177308

    Purpose

    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid

    #221004

    Purpose

    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    CasRx 30nt processed direct repeat with SD1 mutation
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    DR1 A>T

    Promoter

    U6

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPRISM-Cre-cmlc2-TagBFP

    Plasmid

    #117792

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    Cre
    

    Use

    Synthetic Biology

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-Cas9-gRNA-TagBFP2

    Plasmid

    #124774

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, TagBFP2, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS613_pTetR-P2A-BFPnls/sgTelo

    Plasmid

    #108649

    Purpose

    Telomere-targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Telomere-targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-CAG-Lck-mTagBFP2

    Plasmid

    #234537

    Purpose

    to label the membrane of cells with a fluorescent protein (mTagBFP2)

    Depositor

    Hajime Hirase

    Article

    Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

    Insert

    Lck-mTagBFP2
    

    Use

    AAV

    Mutation

    None

    Promoter

    Short CAG

    Available Since

    June 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_ABE8e-SpRY-P2A-BFP_HygroR

    Plasmid

    #213009

    Purpose

    A lentiviral vector used to create stable cell lines expressing the ABE8e-SpRY base editor and BFP

    Depositor

    Richard Sherwood

    Article

    Ryu et al Nat Genet. 2024 Apr 24. doi: 10.1038/s41

    Insert

    ABE8e-SpRY-D10A
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A nickase variant of SpRY

    Promoter

    Ef-1a

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti Lifeact-mTagBFP2 PuroR

    Plasmid

    #101893

    Purpose

    Lentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Promoter

    CMV immediate early

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFA6a-link-yomTagBFP2-Kan

    Plasmid

    #44899

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomTagBFP2
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. dCas9-SunTag(10X)-2A-BFP

    Plasmid

    #227389

    Purpose

    Adhesion module, dCas9-SunTag

    Depositor

    Clifford Brangwynne

    Article

    Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

    Insert

    dCas9-SunTag
    

    Use

    Lentiviral

    Mutation

    Enzymatically dead Cas9

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAW63.YY1.FKBP.knock-in.BFP

    Plasmid

    #104371

    Purpose

    For knocking in FKBP F36V P2A BFP

    Depositor

    Richard Young

    Article

    Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

    Insert

    FKBP12(F36V) -P2A-BFP
    

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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