We narrowed to 3,128 results for: BFP

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pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W

Plasmid

#67974

Purpose

CRISPR gRNA expression vector with an improved scaffold and puro/BFP markers

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKpuro2ABFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

Plasmid

#50946

Purpose

An empty gRNA expression vector

Depositor

Kosuke Yusa

Article

Koike-Yusa et al Nat Biotechnol. 2014 Mar;32(3):267-73. d

Insert

None

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 21, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti-U6-sgRNA-BFP-Puro

Plasmid

#229014

Purpose

Perturb Seq lentiviral sgRNA vector

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Type

Empty backbone

Use

Lentiviral

Available Since

May 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-eBFP2-NLS-pA

Plasmid

#183800

Purpose

AAV vector to express nuclear localized eBFP2 from CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eBFP2-NLS

Use

AAV

Tags

NLS

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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AAVS1-SA-CAG-Pa01-BFP

Plasmid

#234142

Purpose

Perturb Seq landing pad with Pa01 recombinase at the AAVS1 loci

Depositor

Gary Hon

Article

Sivakumar et al Stem Cell Reports. 2025 Dec 9;20(12):102

Insert

Pa01

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES-NRASG12D-BFP

Plasmid

#236202

Purpose

Expresses NRASG12D in mammalian cells

Depositor

Michael Heuser

Article

Mohanty et al Blood Adv. 2025 May 7:bloodadvances.2024

Insert

NRAS (NRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

G12D

Promoter

MSCV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomTagBFP2-Kan

Plasmid

#44899

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomTagBFP2

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pAP215.rAAV.mU6-sgRNA.Handle.EF1a-mTagBFP2

Plasmid

#217635

Purpose

AAV backbone for sgRNA expression. Contains a Lox-flanked handle sequence for Cre-dependent PCR amplification of sgRNAs. Protospacer is cloned between BstXI and BlpI.

Depositor

Martin Kampmann

Article

Ramani et al Nat Neurosci. 2025 Aug 22. doi: 10.1038/

Insert

mU6-sgRNA, Handle sequence, EF1a-mTagBFP2

Use

AAV

Promoter

EF1alpha and mU6

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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AID-mTagBFP2-loxP_myo2_neoR-loxP

Plasmid

#194055

Purpose

Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Tags

codon optimized mTagBFP2

Expression

Worm

Promoter

None

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pC13N-dCas9-BFP-KRAB

Plasmid

#127968

Purpose

constitutive expression of dCas9-BFP-KRAB from the CLYBL locus (Ward lab)

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-dCas9-NLS-BFP-KRAB

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVS1-tet-iCas9-BFP2

Plasmid

#125519

Purpose

all-in-one-inducible Cas9

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Insert

AAVS1-TET-ON 3G-SpCas9-P2A-BFP2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pHR TRE_firefly Luciferase_2A_mCherry-PGK_BFP

Plasmid

#223539

Purpose

Inducible firefly luciferase and mCherry; constitutive BFP

Depositor

Jonathan Brunger

Article

Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

Inserts

Enhanced firefly luciferase
mCherry
TagBFP

Use

Lentiviral and Luciferase

Expression

Mammalian

Available Since

June 17, 2025

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKBFP2AGFP-W

Plasmid

#67980

Purpose

Cas9 activity reporter with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W

Plasmid

#67979

Purpose

Cas9 activity reporter (control) with BFP and GFP.

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKBFP2AGFP cassette, WPRE

Use

Lentiviral

Mutation

Deleted BbsI site within WPRE

Promoter

human U6 promoter, mouse PGK promoter

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A RIGI WT

Plasmid

#167289

Purpose

Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving wild-type RLR sensor RIG-I

Depositor

Agnel Sfeir

Article

Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

Insert

DDX58 (RIGI Human)

Use

Lentiviral

Tags

tagBFP

Expression

Mammalian

Promoter

SFFV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STING

Plasmid

#102586

Purpose

Lentivector to express STING and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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iC13 PB-TO-oNgn2-BFP-puro

Plasmid

#204733

Purpose

Improved Dox-inducible neurogenin-2; Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression. oNGN2 is a Phospho-mutant which avoids inhibition by cdk kinase.

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Insert

Optimized neurogenin-2

Expression

Mammalian

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->sGFP-P2A-BFP

Plasmid

#162234

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-BFP in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and BFP

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Tags

TagBFP and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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