Addgene

We narrowed to 626 results for: LD

Showing: 541 - 560 of 626 results

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pGH020 sgZmat3.2

Plasmid

#227944

Purpose

sgRNA targeting Zmat3, GFP, neomycin resistance, Mammalian expression, Mouse targeting, lentiviral, CRISPR

Depositor

Laura Attardi

Article

Boutelle et al Cell Death Differ. 2025 Apr 22. doi: 10.

Insert

Zmat3.2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiPOLR2D

Plasmid

#202445

Purpose

Expression of a CRISPRi guide targeting POLR2D

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

POLR2D gRNA (POLR2D Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgUBR4

Plasmid

#202442

Purpose

Expression of a guide RNA targeting UBR4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

UBR4 gRNA (UBR4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgUBA6

Plasmid

#202440

Purpose

Expression of a guide RNA targeting UBA6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

UBA6 gRNA (UBA6 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3XHA-p53 QM

Plasmid

#196268

Purpose

Mammalian expression of 3xHA tagged p53 L25Q,Q26S,F53Q,F54S

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Trp53 QM (Trp53 Mouse)

Use

Tags

3xHA

Expression

Mammalian

Mutation

p53 L25Q,Q26S,F53Q,F54S mutant

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-sgZmat3.51.5/Cre

Plasmid

#167855

Purpose

Expresses Cre recombinase and an sgRNA targeting Zmat3

Depositor

Laura Attardi

Article

Bieging-Rolett et al Mol Cell. 2020 Nov 5;80(3):452-469.e9. d

Insert

gRNA targeting Zmat3 (Zmat3 Mouse)

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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Myc-KLC1 delta Tail

Plasmid

#166965

Purpose

Expresses Myc-tagged KLC1 protein (1-495 aa) in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KLC1 (Klc1 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

KLC1 (1-495aa)

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5AR280H

Plasmid

#166957

Purpose

Expresses FLAG-tagged KIF5A R280H protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A-R280H (Kif5a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

KIF5A-R280H

Promoter

CMV

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pMIH-TOMM20-Halo

Plasmid

#111626

Purpose

Fusion protein used to visualise mitochondria upon the addition of Halo specific dye, has hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TOMM20 (Tomm20 Mouse)

Use

Retroviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMIH-TFAM-GFP

Plasmid

#113704

Purpose

Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TFAM (Tfam Mouse)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgATF4-1

Plasmid

#202455

Purpose

Expression of a guide RNA targeting ATF4

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

ATF4 gRNA (ATF4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pLX_314-HRI-V5

Plasmid

#202434

Purpose

Expression of HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-PURO-LYSET-gRNA1

Plasmid

#210489

Purpose

CRISPR pooled KO of human LYSET

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-gRNA1 (LYSET Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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plentiCRISPRv2-PURO-LYSET-gRNA2

Plasmid

#210490

Purpose

CRISPR pooled KO of human LYSET

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

LYSET-gRNA2 (LYSET Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgKCMF1

Plasmid

#202441

Purpose

Expression of a guide RNA targeting KCMF1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

KCMF1 gRNA (KCMF1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLX-N-CTCF-mAID-Halo-donor

Plasmid

#139747

Purpose

Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

Depositor

Robert Tjian

Article

Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

Insert

CTCF CRISPR knock-in (Ctcf Mouse)

Use

CRISPR

Tags

mAID-HaloTag

Expression

Mutation

Promoter

Available since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Voltron-ST

Plasmid

#119036

Purpose

Cre-dependant expression of soma-localized Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Has Service

AAV1

Insert

Voltron-ST

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

hsyn

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV_ACE2-Flag_Hygro

Plasmid

#191998

Purpose

Lentiviral vector to generate flag-tagged ACE2 stable expressing cell line

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

ACE2 (ACE2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-TMPRSS2-TwinStrep_Zeo

Plasmid

#192000

Purpose

Lentiviral vector to generate TwinStrep-tagged TMPRSS2 stable expressing cell line

Depositor

Jan Carette

Article

Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

TMPRSS2

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiSF3B1

Plasmid

#202446

Purpose

Expression of a CRISPRi guide targeting SF3B1

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

SF3B1 gRNA (SF3B1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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