We narrowed to 2,918 results for: gem

1. Torso cDNA Y918F in pGEM

   Plasmid

   #39797

   Depositor

   Norbert Perrimon

   Article

   Gayko et al Proc Natl Acad Sci U S A. 1999 Jan 19;96

   Insert

   Torso (tor Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y918F

   Promoter

   Available since

   Oct. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

   Plasmid

   #111818

   Purpose

   Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Mef2c (Mef2c Mouse)
   Tdtomato
   Gata4 (Gata4 Mouse)
   Tbx5
   

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-U6-Chimeric_BB-CBh-hSpCas9-hGem(1/110)

   Plasmid

   #71707

   Purpose

   A human codon-optimized SpCas9 fused to first 110 amino acids of human GEMININ and chimeric guide RNA expression plasmid

   Depositor

   Giulio Draetta

   Article

   Gutschner et al Cell Rep. 2016 Feb 3. pii: S2211-1247(16

   Insert

   humanized S. pyogenes Cas9 fused to human Geminin (GMNN Human)
   

   Use

   CRISPR

   Tags

   3xFLAG and hGEM(1/110)

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available since

   Feb. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSB-CMV-MCS-Puro GEM-LTB4

   Plasmid

   #202641

   Purpose

   Expresses the LTB4 sensor GEM-LTB4 in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   GEM-LTB4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAAVS1_EF1a-GEM2-Halo-FKBP-IRES-Blast

   Plasmid

   #197066

   Purpose

   Donor plasmid for human AAVS1 locus knock-in, constitutive expression of GEM2-Halo-FKBP

   Depositor

   Julia Mahamid

   Article

   Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

   Insert

   Encapsulin
   

   Use

   CRISPR

   Tags

   FKBP and HaloTag

   Expression

   Mammalian

   Mutation

   Codon-optimised for human cell expression

   Promoter

   Available since

   Sept. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSB-CMV-MCS-Puro GEM-LTB4mut

   Plasmid

   #202642

   Purpose

   Expresses the LTB4-insensitive control sensor GEM-LTB4mut in mammalian cells

   Depositor

   Balázs Enyedi

   Article

   Tamas et al Nat Commun. 2023 Aug 1;14(1):4610. doi:

   Insert

   GEM-LTB4mut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

   Plasmid

   #165489

   Purpose

   Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

   Depositor

   Thomas Oertner

   Article

   Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

   Insert

   2xLyn-ERex-Venus-bPAC(F198Y)
   

   Use

   Cdna expression, xenopus oocyte

   Tags

   Venus

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

   Plasmid

   #119864

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Use

   Tags

   HA-tagged biotin ligase (BirA), Thosea asigna vir…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

   Plasmid

   #140200

   Purpose

   Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   dLbCas12a crRNA scaffold

   Expression

   Mutation

   Promoter

   PgpdA

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid

    #140204

    Purpose

    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Step 1 of cloning c…

    Tags

    Expression

    Mutation

    Promoter

    Aspergillus fumigatus U3 (RNAPIII).

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid

    #225443

    Purpose

    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.

    Depositor

    Diego Orzaez

    Insert

    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLXB3o1 HygR-MAR10-gemiGFP-MAR10 (GB4684)

    Plasmid

    #225439

    Purpose

    Module for stable transformation of Geminino 1.0-eGFP flanked by two MAR10 insulators and next to a Hygromycin resistance cassette.

    Depositor

    Diego Orzaez

    Insert

    HygR-MAR10-gemiGFP-MAR10
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Plasmid

    #224755

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131C/C159A/C219A/C365A/C399A

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEMHE:mTREK-1 (S131A/C159A/C219A/C365A/C399A)

    Plasmid

    #224756

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131A/C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S131A/C159A/C219A/C365A/C399A

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEM-human TCR zeta/2470

    Plasmid

    #11507

    Depositor

    Allan Weissman

    Article

    Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

    Insert

    TCR zeta (CD247 Human)
    

    Use

    General cloning

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

17. 3155 pGEM-4Z Bcl-2 cDNA G145A

    Plasmid

    #8751

    Depositor

    Stanley Korsmeyer

    Article

    Yin et al Nature 1994 May 26;369(6478):321-3.

    Insert

    Bcl-2 (BCL2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glycine 145 mutated to Alanine

    Promoter

    Available since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pGEM-rat FceRI beta cDNA

    Plasmid

    #8374

    Depositor

    Jean Kinet

    Article

    Kinet et al Proc Natl Acad Sci U S A 1988 Sep;85(17)

    Insert

    rat Fc epsilon RI beta chain (Ms4a2 Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2005

    Availability

    Academic Institutions and Nonprofits only

19. pGEM-mouse TCR zeta/2451

    Plasmid

    #11506

    Depositor

    Allan Weissman

    Article

    Weissman et al Science. 1988 Feb 26. 239(4843):1018-21.

    Insert

    TCR zeta (Cd247 Mouse)
    

    Use

    General cloning

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    March 8, 2006

    Availability

    Academic Institutions and Nonprofits only

20. XE169 mouse Wnt-1-myc-pGEM4

    Plasmid

    #16855

    Depositor

    Randall Moon

    Article

    McMahon et al Cell. 1989 Sep 22;58(6):1075-84. doi: 10

    Insert

    Wnt-1-myc (Wnt1 Mouse)
    

    Use

    Xenopus expression

    Tags

    Myc

    Expression

    Mutation

    Promoter

    Available since

    Sept. 13, 2012

    Availability

    Academic Institutions and Nonprofits only