We narrowed to 1,028 results for: hug;

1. pM-mTFP1

   Plasmid

   #228621

   Purpose

   Mammalian expression of mTFP1 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mTFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pM-CyOFP1

   Plasmid

   #228623

   Purpose

   Mammalian expression of CyOFP1 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   CyOFP1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pM-mOrange2

   Plasmid

   #228627

   Purpose

   Mammalian expression of mOrange2 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mOrange2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pM-mRuby3

   Plasmid

   #228628

   Purpose

   Mammalian expression of mRuby3 and extra insert, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET30b jGCaMP8f L27A

   Plasmid

   #204138

   Purpose

   Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f L27A
   

   Use

   Tags

   N-terminal His tag

   Expression

   Bacterial

   Mutation

   Leucine 27 changed to Alanine

   Promoter

   T7

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-CMV-jGCaMP8f L27A

   Plasmid

   #204140

   Purpose

   Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

   Depositor

   Katalin Torok

   Article

   Tran et al Front Cell Neurosci. 2023 May 18;17:1155

   Insert

   jGCaMP8f L27A
   

   Use

   Tags

   N-terminal His tag

   Expression

   Mammalian

   Mutation

   Leucine 27 changed to Alanine

   Promoter

   CMV

   Available since

   Dec. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Ash1-5p

   Plasmid

   #169712

   Purpose

   Bacterial Expression of truncated yeast Ash1 including encoding amino acids 420-480 with phosphorylation sites 429 and 470 removed

   Depositor

   Tanja Mittag

   Article

   Martin et al J Am Chem Soc. 2016 Nov 30;138(47):15323

   Insert

   Ash1 (ASH1 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Truncation including encoding amino acids 420-480…

   Promoter

   T7

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBH-Cas9-Suntag-BB_BbsI

   Plasmid

   #164805

   Purpose

   REDIT backbone for SunTag Cas9, pU6-gRNA-backbone(BbsI)-CBH-SpCas9-Suntag-T2A-EBFP

   Depositor

   Le Cong

   Article

   Wang et al Nucleic Acids Res. 2021 Apr 6;49(6):e36.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJC2-GPT1-3xFLAG

   Plasmid

   #163446

   Purpose

   lentiviral overexpression vector for human GPT1

   Depositor

   Jason Cantor

   Article

   Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

   Insert

   GPT1 (GPT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   See: comments

   Promoter

   CMV

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCHR-CD5HA2-P2A-smAID-mClover

    Plasmid

    #110658

    Purpose

    Lentiviral vector expressing cell surface HA-tag and monomeric GFP N-terminaly fused with auxin inducible degron

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CD5HA2-P2A-AID-mClover
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5/FRT-DEST EVC2ala7-TEV-YFP-Flag

    Plasmid

    #41013

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1189-1192 to Alanine

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag

    Plasmid

    #41012

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1185-1188 to Alanine

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag

    Plasmid

    #41011

    Depositor

    Rajat Rohatgi

    Article

    Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

    Insert

    Ellis van Creveld syndrome 2 (EVC2 Mouse)
    

    Use

    Tags

    TEV-YFP-Flag

    Expression

    Mammalian

    Mutation

    changed amino acids 1183-1186 to Alanine

    Promoter

    Available since

    Nov. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA-K-GECO1

    Plasmid

    #105865

    Purpose

    Mammalian cell expression plasmid for red fluorescent Ca2+ indicator K-GECO1

    Depositor

    Robert Campbell

    Article

    Shen et al BMC Biol. 2018 Jan 16;16(1):9. doi: 10.1

    Insert

    K-GECO1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. NowGFP /pQE-30

    Plasmid

    #74749

    Purpose

    NowGFP gene for bacterial expression

    Depositor

    Konstantin Lukyanov

    Article

    Sarkisyan et al Biophys J. 2015 Jul 21;109(2):380-9. doi

    Insert

    NowGFP
    

    Use

    Tags

    HisTag (with a mutation but still working)

    Expression

    Bacterial

    Mutation

    WasCFP with substitutions E6K, L42M, T43S, V68M, …

    Promoter

    T5

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. CaMKAR

    Plasmid

    #205315

    Purpose

    Fluorescent Reporter for CaMKII Activity

    Depositor

    Mark Anderson, Elizabeth Luczak

    Article

    Reyes Gaido et al Sci Transl Med. 2023 Jun 21;15(701):eabq

    Insert

    CaMKAR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR1a-tagRFP-KDEL

    Plasmid

    #114177

    Purpose

    Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Gateway entry clone

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiPGK Blast DEST ERKKTRmRuby2

    Plasmid

    #90231

    Purpose

    Lentiviral vector to express ERK KTR mRuby2 under PGK promoter (With Blasticidin Resistance)

    Depositor

    Markus Covert

    Article

    Kudo et al Nat Protoc. 2018 Jan;13(1):155-169. doi:

    Insert

    ERK Kinase Translocation Reporter
    

    Use

    Lentiviral

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    March 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENTR-ERKKTRClover

    Plasmid

    #59138

    Purpose

    ORF encoding ERK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    ERK Kinase Translocation Reporter (MAPK1 Mouse, Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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