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MELK E6.2 gRNA

Plasmid

#90767

Purpose

3rd generation lentiviral gRNA plasmid targeting human MELK

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MELK (Guide Designation E6.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_Mito

Plasmid

#65669

Purpose

Expresses Mito-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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TAL3420

Plasmid

#47139

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-def6a-left (def6a Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
CDCA5 C9.4 gRNA

Plasmid

#90600

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDCA5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CDCA5 (Guide Designation C9.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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TAL3219

Plasmid

#42682

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg5-right (atg5 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
TAL3217

Plasmid

#42680

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg12-right (atg12 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
TAL3218

Plasmid

#42681

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-atg5-left (atg5 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
PPP2R2C B7.5 gRNA

Plasmid

#90849

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R2C

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R2C (Guide Designation B7.5)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEM-Cas9noSsrA

Plasmid

#102285

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to remove the ssrA tag from Cas9.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9

Use

CRISPR

Expression

Bacterial

Mutation

removed ssrA tag

Promoter

pTet

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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HDGF A7.3 gRNA

Plasmid

#90708

Purpose

3rd generation lentiviral gRNA plasmid targeting human HDGF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

HDGF (Guide Designation A7.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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HDGF A8.3 gRNA

Plasmid

#90709

Purpose

3rd generation lentiviral gRNA plasmid targeting human HDGF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

HDGF (Guide Designation A8.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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NXT1 A5.3 gRNA

Plasmid

#90814

Purpose

3rd generation lentiviral gRNA plasmid targeting human NXT1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NXT1 (Guide Designation A5.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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KIF11 F6.3 gRNA

Plasmid

#90717

Purpose

3rd generation lentiviral gRNA plasmid targeting human KIF11

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

KIF11 (Guide Designation F6.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TOP2A G12.2 gRNA

Plasmid

#90915

Purpose

3rd generation lentiviral gRNA plasmid targeting human TOP2A

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TOP2A (Guide Designation G12.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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NDC80 F12.1 gRNA

Plasmid

#90787

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F12.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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NDC80 F11.1 gRNA

Plasmid

#90786

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F11.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H1.1 gRNA

Plasmid

#90886

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H1.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H2.1 gRNA

Plasmid

#90887

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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ASF1B A4.2 gRNA

Plasmid

#90535

Purpose

3rd generation lentiviral gRNA plasmid targeting human ASF1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ASF1B (Guide Designation A4.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ASF1B A3.2 gRNA

Plasmid

#90534

Purpose

3rd generation lentiviral gRNA plasmid targeting human ASF1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ASF1B (Guide Designation A3.2)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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