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We narrowed to 6,252 results for: nls

Showing: 5601 - 5620 of 6252 results

  1. pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)

    Plasmid
    #61364
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61364-plasmid-map-sequence-id-99800

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  2. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  3. pcDNA-dCas9-p300 Core (1396/1397 SY/WW)

    Plasmid
    #61363
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation 1396/1397 SY/WW) driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, Human, S. pyogenes)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1396/1397 SY/WW m…
    Promoter
    CMV
    Available Since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61363-plasmid-map-sequence-id-99798

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  4. Bxb1-GT-Dox-AIO-TetOn_Ngn2-ISL1-LHX3_Down-Tandem

    Plasmid
    #241396
    Purpose
    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of Ngn2, ISL1 and LHX3
    Depositor
    Richard Davis
    Article
    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    TRE-Ngn2-ISL1-LHX3; rtTA-T2A-mTagBFP2 (ISL1, LHX3, NEUROG2 Human)
    Tags
    NLS
    Expression
    Mammalian
    Promoter
    TRE; CAG
    Available Since
    Oct. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    241396-plasmid-map-sequence-id-489344

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  6. PB-TO-hNIL

    Plasmid
    #172113
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Article
    Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1
    Inserts
    hNGN2 (NEUROG2 Human)
    hISL1 (ISL1 Human)
    hLHX3 (LHX3 Human)
    puroR-T2A-mycNLS-mTagBFP2
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Promoter
    EF1a and TRE3G
    Available Since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    172113-plasmid-map-sequence-id-359519

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  7. P3-dCas9-Tet1-GFP-Puro

    Plasmid
    #190728
    Purpose
    Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)
    Depositor
    Maja Jagodic
    Article
    Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:
    Insert
    dCas9-tet1
    Use
    CRISPR
    Tags
    3XFLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Changed Pro434 codon from CCG to CCC and Gly468 f…
    Promoter
    pCAG
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    190728-plasmid-map-sequence-id-381333

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  8. T2A Split/Link TurboID Kit

    Plasmid Kit
    #1000000249
    Purpose
    The T2A Split/Link TurboID Kit uses a sequential Golden Gate assembly method and can be used to assemble mammalian expression vectors for proximity labeling experiments.
    Depositor
    Sven Eyckerman
    Available Since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only

  10. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid
    #223065
    Purpose
    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    Use
    CRISPR
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Three regions of SpCas9 encode type IIS restricti…
    Promoter
    Dual T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223065-plasmid-map-sequence-id-447614

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  12. PABE-7-NG

    Plasmid
    #138142
    Purpose
    Targeted A to G in rice protoplasts
    Depositor
    Caixia Gao
    Insert
    wtTadA-TadA7.10-nCas9-NG-3XNLS
    Expression
    Plant
    Available Since
    Sept. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    138142-plasmid-map-sequence-id-311738

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  13. pPD8_33

    Plasmid
    #1468
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1468-plasmid-map-sequence-id-79

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  14. pPD18_32

    Plasmid
    #1435
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1435-plasmid-map-sequence-id-46

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  15. pPD16_43

    Plasmid
    #1433
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1433-plasmid-map-sequence-id-44

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  16. pPD26_77

    Plasmid
    #1441
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1441-plasmid-map-sequence-id-52

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  17. pPD90_23

    Plasmid
    #1469
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1469-plasmid-map-sequence-id-80

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  18. pDD16_01

    Plasmid
    #1428
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1428-plasmid-map-sequence-id-39

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  19. pPD16_51

    Plasmid
    #1434
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1434-plasmid-map-sequence-id-45

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  20. pPD21_28

    Plasmid
    #1438
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Tags
    IVSA, SV40NLS, lacZN, lacZC
    Expression
    Worm
    Available Since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1438-plasmid-map-sequence-id-49

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Showing: 5601 - 5620 of 6252 results