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pEJS1831 All-in-one AAV-U1a-NmeABE-8e-2xBPSV40-miniU6-Fah

Plasmid

#199264

Purpose

Single AAV vector for expressing N-terminal fusion Nme2Cas9-ABE8e and one miniU6 driven sgRNA targeting the point mutation in the mouse Fah gene of a HT1 mouse model

Depositor

Erik Sontheimer

Article

Zhang et al GEN Biotechnol. 2022 Jun 1;1(3):285-299.

Insert

NmeABE8e

Use

AAV and CRISPR

Tags

NLS

Expression

Mammalian

Available Since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVss-U6-sgHTT51-7sk-Cas9

Plasmid

#190901

Purpose

AAV-KamiCas9 vector expressing sgGFP and mouse HTT sgRNA

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

sgRNA (Htt Mouse)

Use

AAV and CRISPR

Promoter

U6 and 7sk

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1

Plasmid

#190902

Purpose

AAV vector expressing thew GFP reporter gene and human sgHTT

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

GFP and sgHTT (HTT Human)

Use

AAV and CRISPR

Promoter

EFS and U6

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2ss-EFS-GFP-synPolyA-U6-sgHTT1-U6-sgCas9

Plasmid

#190903

Purpose

AAV-KamiCas9 vector expressing expressing thew GFP reporter gene and sgHTT and sgCas9

Depositor

Nicole Déglon

Article

Duarte et al Mol Ther Methods Clin Dev. 2023 Mar 27;2

Insert

GFP, sgHTT and sgCas9 (HTT Human)

Use

AAV and CRISPR

Promoter

EFS and U6

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV2-UbC-3xNLS-mScarlet-I

Plasmid

#191099

Purpose

To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods

Depositor

Murray Blackmore, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

3xNLS-mScarlet-I

Use

AAV and Synthetic Biology

Tags

Three repeats of the nuclear localizzation seque…

Expression

Mammalian

Mutation

optimized to human codon usage

Promoter

UbC

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-1

Plasmid

#180400

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-1 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-2

Plasmid

#180401

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-2 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shSrpk3-3

Plasmid

#180402

Purpose

Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shSrpk3-3 (Srpk3 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-2

Plasmid

#180392

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-2 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shStk16-3

Plasmid

#180393

Purpose

Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shStk16-3 (Stk16 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-1

Plasmid

#180394

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-1 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-1 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-2

Plasmid

#180395

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-2 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-2 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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AAV.CBA.YFP.miR-E_shTgm5-3

Plasmid

#180396

Purpose

Producing AAV that encodes mouse Tgm5 shRNA-3 with miR-E backbone

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

shTgm5-3 (Tgm5 Mouse)

Use

AAV and RNAi

Promoter

CBA

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pGW02 AAV-TBG-Cre-sgP53-sgRNA

Plasmid

#192162

Purpose

AAV-TBG-Cre-sgP53-sgRNA

Depositor

Sidi Chen

Article

Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

Type

Empty backbone

Use

AAV

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph18_eGFP_CCR5TC

Plasmid

#187443

Purpose

Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph18

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_Syn_Xph15_eGFP_CCR5TC

Plasmid

#187442

Purpose

Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph15

Use

AAV

Tags

eGFP

Expression

Mammalian

Promoter

Synapsin

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-GCaMP6f

Plasmid

#178727

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GCaMP6f

Plasmid

#178729

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-GCaMP6f

Plasmid

#178731

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6-GCaMP6f

Plasmid

#178732

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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